JMASM37: Simple Response Surface Methodology Using RSREG (SAS)

Amir, Wan Muhamad; Shafiq, Mohamad; Mokhtar, Kasypi; Aleng, Nor Azlida; Rahim, H. Abdul; Ali, Zalila

doi:10.22237/jmasm/1462077780

Cited by 6 publications

(3 citation statements)

References 4 publications

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“…Also, in order to evaluate the synergistic effects of both glucose, sodium acetate and the effects of their high concentration on Y. lipolytica growth and PHB content, response surface methodology RSM analysis was used [30]. RSM analysis was done using SAS RSREG procedure [31]. For the statistical analysis, linear, seconddegree polynomials, and quadratic models were used to describe the relationship between the input variables and the responses.…”

Section: Discussionmentioning

confidence: 99%

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered <i>Yarrowia Lipolytica</i> Using Co-Substrate Strategy

Tourang,

Sarkhosh,

Chen

2023

Preprint

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High production cost is one of the major factors that limit the market growth of polyhydroxyalkanoates (PHAs) as a biopolymer. Improving PHA synthesis performance and utilizing low grade feedstock are two logical strategies for reducing the cost. As an oleaginous yeast, Y. lipolytica has a high carbon flux through acetyl-CoA (main PHB precursor) which makes it a desired cell factory for PHB biosynthesis. Two different metabolic pathways introduced into Y. lipolytica PO1f in this study for enhancing PHB biosynthesis, along with glucose and acetate as co-substrates. Results showed that NBC pathway led to more than 11% PHB accumulation, while there was non-detectable PHB accumulations using ABC pathway. Further modifications were done using peroxisomal compartmentalization and gene-dose overexpression. Final strain showed up to 41% PHB of CDW with a growth rate of 0.227 h-1. Low growth rate was observed using acetate or glucose as sole carbon and energy source. The co-substrate strategy showed compensatory effect on overcoming inhibitory and toxic effects of both substrates at high concentrations. Cultivating the engineered strain in optimal co-substrate condition predicted by RSM led to 83.4 g/L biomass concentration and 31.7 g/L PHB.

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Section: Discussionmentioning

confidence: 99%

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered <i>Yarrowia Lipolytica</i> Using Co-Substrate Strategy

Tourang,

Sarkhosh,

Chen

2023

Preprint

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show abstract

“…Also, to evaluate the synergistic effects of both glucose and sodium acetate and the effects of their high concentrations on Y. lipolytica growth and PHB content, the response surface methodology (RSM) analysis was performed [29]. RSM analysis was conducted via the SAS RSREG procedure [30]. For statistical analysis, linear, second-degree polynomials and quadratic models were applied to describe the relationship between the input variables and the responses.…”

Section: Discussionmentioning

confidence: 99%

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered Yarrowia lipolytica Strain Using Co-Substrate Strategy

Tourang,

Xiong,

Sarkhosh

et al. 2023

Fermentation

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High production cost is one of the major factors that limit the market growth of polyhydroxyalkanoates (PHAs) as a biopolymer. Improving PHA synthesis performance and utilizing low-grade feedstocks are two logical strategies for reducing costs. As an oleaginous yeast, Y. lipolytica has a high carbon flux through acetyl-CoA (the main PHB precursor), which makes it a desired cell factory for PHB biosynthesis. In the current study, two different metabolic pathways (NBC and ABC) were introduced into Y. lipolytica PO1f for synthesizing PHB. Compared to the ABC pathway, the NBC pathway, which includes NphT7 to redirect the lipogenesis pathway and catalyze acetoacetyl-CoA synthesis in a more energy-favored reaction, led to PHB accumulation of up to 11% of cell dry weight (CDW), whereas the ABC pathway resulted in non-detectable accumulations of PHB. Further modifications of the strain with the NBC pathway through peroxisomal compartmentalization and gene dose overexpression reached 41% PHB of CDW and a growth rate of 0.227 h−1. A low growth rate was observed with acetate as the sole source of carbon and energy or glucose as the sole substrate at high concentrations. Using a co-substrate strategy helped overcoming the inhibitory and toxic effects of both substrates. Cultivating the engineered strain in the optimal co-substrate condition predicted by response surface methodology (RSM) led to 83.4 g/L of biomass concentration and 31.7 g/L of PHB. These results offer insight into a more cost-effective production of PHB with engineered Y. lipolytica.

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“…It involves the use of a sequence of designed experiments to obtain an optimal response through linear models and second-degree polynomials. It is useful also for modeling and analysis of problems in which a response of interest is influenced by some variables (Ba et al, 2007) (Khoshnamvand et al, 2018) (Kumari et al, 2019) (Oehlert et al, 2000) (Amir et al, 2016) (Ngo et al, 2012). In NPs field, RSM have been used for modeling the NPS production process as well as optimization of the parameter affecting the production processes (Beg et al, 2002) (Gadekar et al, 2019) (Nikaeen et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Modeling the Processes of Green Magnetite Nanoparticle: Synthesis, Photo Catalysis Tendency and Kinetics of Removing Organic Dye from Aqueous Solutions

2023

EAJSE

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Green magnetite nanoparticles (NPs) are synthesized, characterized and employed for degradation Methylene Blue (MB) from aqueous solutions. The effect of the concentrations of the NPs and MB on NPs yield and removal efficiency is optimized and modeled using two factorial central composite experimental design. The analysis of variance confirmed that the concentration of iron metal salts seemed more significant than plant extract. The developed mathematical model is estimated with high R2 reflecting its accuracy. The results proved that the removal efficiency of MB increases up to an optimum of 82.07 % when using 0.17 g of the nano photo catalyst versus 10.8 ppm of MB with sunlight irradiation time of 200 min. The dye degradation kinetic results revealed that photo catalytic degradation follow pseudo-first-order model. Response Surface Methodology proved as an efficient tool for optimization and modeling the processes of NPs production and removing of organic pollutants from aqueous solutions.

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JMASM37: Simple Response Surface Methodology Using RSREG (SAS)

Cited by 6 publications

References 4 publications

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered <i>Yarrowia Lipolytica</i> Using Co-Substrate Strategy

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered <i>Yarrowia Lipolytica</i> Using Co-Substrate Strategy

Polyhydroxybutyrate (PHB) Biosynthesis by an Engineered Yarrowia lipolytica Strain Using Co-Substrate Strategy

Optimization and Modeling the Processes of Green Magnetite Nanoparticle: Synthesis, Photo Catalysis Tendency and Kinetics of Removing Organic Dye from Aqueous Solutions

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