Since it is indispensable for organizations to employ and retain quality human resources (HRs) and talents for their effective operation and continuous growth, it is imperative and relevant to investigate into the HR retention practices and strategies. Hence, the paper examines the influence of human resource management practices on employee retention and the moderating effect of management hierarchy, age group and gender on HR retention factors in hospitality industry of Nepal. The study employed a cross-sectional descriptive survey design with a sample of 292 respondents from six out of eight five-star hotels of Nepal stationed in the capital Valley of Kathmandu. Using correlation analysis, Jonckheere-Terpstra test, as well as post hoc and Mann-Whitney U tests, the study discovered that rewards and compensation help retain entry level employees, while employer branding is a strong retention measure for those on higher hierarchies. Likewise, balanced work life and employer branding help retain higher age group employees, while career growth is the strongest and balanced work life the weakest measure to retain male hotel employees in Nepal.