Joint effects of CSN3 and LGB genes on milk quality and coagulation properties in Czech Fleckvieh

Matéjicek, Annick; Matějíčková, J.; Štípková, M.; Hanuš, Oto; Genčurová, Václava; Kyselová, J; Němcová, Eva; Kott, T.; Šefrová, J.; Krejčová, M.; Melčová, S.; Hölzelová, I.; Bouška, J.; Frelich, J.

doi:10.17221/363-cjas

Cited by 19 publications

(14 citation statements)

References 18 publications

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“…The CSN2 gene polymorphism has also been shown to be associated with specific milk performance parameters (Kučerová et al, 2006). The MAS (Marker-Assisted Selection) process can play an important role in increasing the quality of milk/dairy products and in acquiring specific milk properties as indicated by results described by many authors (Hanuš et al, 1995;Michalcová and Krupová, 2007;Hradecká et al, 2008;Matějíček et al, 2008). The challenge for scientists remains the development of new, more powerful and more reliable methods which will give breeders new options.…”

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confidence: 99%

TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

Manga¹,

Dvořák²

2010

CZECH J ANIM SCI

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Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allele frequencies were as follows: A1A1-0.20, A1A2-0.51, A2A2-0.29; A1-0.45, A2-0.55.

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confidence: 99%

TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

Manga¹,

Dvořák²

2010

CZECH J ANIM SCI

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“…However, there is a need for more information regarding the effect of other PRKAG3 polymorphisms on meat quality. Moreover, as genes act in mutual interactions (Szyda et al, 2006;Matějíček et al, 2008) we were interested in a comparison of the impact of two genes proved important for meat quality. Thus the aim of the present study was to associate PRKAG3 genotypes (Q/R-I/V, Q/R-V/V, R/R-I/I, R/R-I/V and R/R-V/V) with meat quality traits and to relate their impact to one of the RYR1 genotypes (N/N or N/n).…”

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confidence: 99%

Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Škrlep¹,

Kavar²,

Čandek-Potokar³

2010

Czech J. Anim. Sci.

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ABSTRACT:The effect of polymorphisms at PRKAG3 (R200Q and I199V) and RYR1 (R615C) genes on carcass traits and meat quality was examined in a sample of 257 commercial pigs, crosses of Landrace × Large White as maternal line and Pietrain (N = 96), Pietrain × Landrace (N = 42) or Pietrain × Hampshire (N = 119) as paternal line. Pigs were genotyped (PCR-RFLP) and traits of interest were measured (which included carcass and ham weight, measurements of fatness, meatiness, ultimate pH, colour parameters and drip loss). The observed genotype frequencies at PRKAG3 gene were 9.7%, 38.9%, 32.7%, 6.2% and 12. 5% for R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/Vand Q/R-V/V genotype, respectively. RYR1 genotype frequencies were 57.2% for N/N and 42.8% for N/n genotype. Studied polymorphisms exhibited a significant effect on meat quality, but mainly an insignificant effect on carcass traits. No significant interaction between PRKAG3 and RYR1 was found. Carriers of RYR1 mutant allele "n" had less intense longissimus dorsi muscle colour (subjective score, Minolta L* and b*) and higher drip loss. Regarding PRKAG3, the ultimate pH decreased and Minolta L*, a*, b* and drip loss increased in the following order: R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V and Q/R-V/V, according to the presence of 199I and absence of 200Q alleles. The study shows that the I199V polymorphism is an important source of variation in pigs free of 200Q. In particular the 199I proves beneficial for meat quality. The results of combining the RYR1 and PRKAG3 genotypes indicate that R/R-I/I genotype could be used in counterbalancing the negative effects of "n" allele on meat quality.

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“…Mainly, the ability to produce milk and its processing qualities are already determined by genotype of the cow (Bayram et al, 2009;Matějíček et al, 2008). Other factors, which are important for the characteristics mentioned above, are stage of lactation and parity (Summer et al, 2003) and feed (Davies and White, 1958).…”

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confidence: 99%

Influence of Summer Temperatures on Milk Content and Technological Properties of Three Cow Breeds Kept Under Identical Conditions

Navrátil

Falta

Müller

et al. 2017

AGR

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The aim of this study was to assess the influence of summer temperatures on the milk content and technological properties for three different cow breeds. The experiment took place between June and August 2015. We observed 18 dairy cows, kept in identical conditions. We used cows of 3 breeds: Czech Fleckvieh (C), Holstein (H) and Ayrshire (A). Samples were taken weekly. The analyzed parameters were: total yield per observed period, milk content, density, active and titrable acidity, rennet coagulation time (RCT) and curd class quality. Average daily yield was 22.8 (A), 28.1 (H) and 24.4 (C) liters. All cows were at the same lactation (2nd). All breeds showed an increase (from 12.74% to 12.24%) in dry matter. Titrable acidity was significantly (P < 0.05) increasing and active acidity was decreasing for all three breeds. When it comes to technological properties Czech Fleckvieh and Holstein showed a decrease in RCT and curd quality, and Ayrshire showed an increase in both RCT and curd quality. This can point to the fact that, though these three breeds are different, some characteristics (dry matter, titrable and active acidity) of their production are affected by the high summer temperatures. RCT values indicate that Czech Fleckvieh is more adaptable to high temperatures.

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Joint effects of CSN3 and LGB genes on milk quality and coagulation properties in Czech Fleckvieh

Cited by 19 publications

References 18 publications

TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Influence of Summer Temperatures on Milk Content and Technological Properties of Three Cow Breeds Kept Under Identical Conditions

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