Joint Report of the Third International Workshop on Canine Immunogenetics

Deeg, H. Joachim; Rf, Raff; Grosse‐Wilde, H.; Bijma, Amelie M.; Buurman, W. A.; Doxiadis, Ilias; Kolb, Hans‐Jochem; Krumbacher, K.; Ladiges, Warren; Losslein, K. L.; Schoch, Gary; Westbroek, D. L.; Bull, Robert W.; Storb, Rainer

doi:10.1097/00007890-198601000-00022

Cited by 41 publications

(19 citation statements)

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“…All dogs were healthy, regularly dewormed, and vaccinated against distemper, leptospirosis, parvovirus, and canine hepatitis. DLA were determined using alloimmune antisera against DLA-A and DLA-B Ags as defined in the Canine Leukocyte Ag Workshop (11). Cells were incubated with polyclonal DLA antisera and lysed through the addition of complement, and the percentage of dead cells was analyzed in FACS with propidium iodide staining.…”

Section: Dogs Dla-typing Bone Marrow and Peripheral Blood Samplesmentioning

confidence: 99%

Minor Histocompatibility Antigens on Canine Hemopoietic Progenitor Cells

Weber

Lange

Günther

et al. 2003

The Journal of Immunology

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Adoptive immunotherapy with CTL against minor histocompatibility Ags (mHA) provides a promising way to treat leukemia relapse in allogeneic chimeras. Here we describe the in vitro generation of CTL against mHA in the dog. We tested their inhibitory effect on the growth of hemopoietic progenitor cells stimulated by hemopoietic growth factors in a 4-day suspension culture. CTL were produced by coculture of donor PBMC with bone marrow-derived dendritic cells (DCs). These DCs were characterized by morphology, high expression of MHC class II and CD1a, and the absence of the monocyte-specific marker CD14. Characteristically these cells stimulated allogeneic lymphocytes (MLR) and, after pulsing with a foreign Ag (keyhole limpet hemocyanin), autologous T cells. CTL were generated either ex vivo by coculture with DCs of DLA-identical littermates or in vivo by immunization of the responder with DCs obtained from a DLA-identical littermate. In suspension culture assays the growth of hemopoietic progenitor cells was inhibited in 53% of DLA-identical littermate combinations. In canine families mHA segregated with DLA as restriction elements. One-way reactivity against mHA was found in five littermate combinations. In two cases mHA might be Y chromosome associated, in three cases autosomally inherited alleles were detected. We conclude that CTL can be produced in vitro and in vivo against mHA on canine hemopoietic progenitor cells using bone marrow-derived DCs.

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Section: Dogs Dla-typing Bone Marrow and Peripheral Blood Samplesmentioning

confidence: 99%

Minor Histocompatibility Antigens on Canine Hemopoietic Progenitor Cells

Weber

Lange

Günther

et al. 2003

The Journal of Immunology

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“…The study included one representative dog of DLA-D types Dw3, Dw8, D4, D7, and DIO and two members of DLA-D types Dw l , Dw4 , D1, D6, D8, D9 , and D16. The MLC typing was done by one-way MLC tests as described (Deeg et al 1986). …”

Section: Dogsmentioning

confidence: 99%

“…The work summarized in the first, second, and third international workshops on canine immunogenetics established that the MHC system of the dog (termed DLA) is similar to HLA in man. The DLA can be divided into three serologically defined loci, designated DLA-A, DLA-B, and DLA-C (Bull et al 1987), and a fourth region (termed DLA-D) defined by mixed leucocyte culture (MLC) reactivity (Deeg et al 1986). Allelic polymorphism within the DLA complex has been previously studied by serology (Bull et al 1987), MLC (Deeg et al 1986), and by restriction fragment length polymorphism (RFLP) analysis (Sarmiento and Storb 1988a, b).…”

Section: Introductionmentioning

confidence: 99%

“…The DLA can be divided into three serologically defined loci, designated DLA-A, DLA-B, and DLA-C (Bull et al 1987), and a fourth region (termed DLA-D) defined by mixed leucocyte culture (MLC) reactivity (Deeg et al 1986). Allelic polymorphism within the DLA complex has been previously studied by serology (Bull et al 1987), MLC (Deeg et al 1986), and by restriction fragment length polymorphism (RFLP) analysis (Sarmiento and Storb 1988a, b). The genomic hybridization studies have indicated that the DLA-D region contains at least five A genes (including DRA, two DQA, DPA, and DNA genes) and seven B genes (including two DRB, two DQB, two DPB, and DOB genes).…”

Section: Introductionmentioning

confidence: 99%

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Allelic variation in theDR subregion of the canine major histocompatibility complex

Sarmiento

Storb

1990

Immunogenetics

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Allelic variation in the DR subregion of the canine major histocompatibility complex (DLA) has been analyzed by nucleic acid sequencing of cDNA clones of DRB genes amplified in vitro by the reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence analysis of a panel of 19 homozygous typing cell dogs representing 12 different DLA-D types (defined by mixed leucocyte reaction) demonstrated the presence of one expressed DRB locus with at least nine distinct alleles in the dog. Unique DLA-DRB alleles were found in the DLA-D types Dw1, Dw3, Dw4, Dw8 (workshop assignments) and D4, D6, D7, D8, and D9 (Seattle assignments). In contrast, the DRB genes of the remaining three DLA-D types (D1, D10, and D16) were identical to those of Dw3/Dw4 (for D1), Dw8 (for D10), and D6 (for D16). The nucleotide sequences of all nine DLA-DRB alleles were typical of functional major histocompatibility complex (MHC) class II beta chains and contained three allelic hypervariable regions (HVRs) in the beta 1 domain at positions 8-16, 26-39, and 57-74. At each variable residue, two to five amino acid substitutions were found. The most polymorphic residues were located at positions 37 (with five amino acid substitutions), 11, 13, 28, and 71 (each with four substitutions). The DLA-DRB alleles had 96%-99% overall nucleotide sequence similarity and 93%-99% amino acid sequence similarity with each other. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the DLA-DRB alleles into three major allelic groups which may represent the canine counterparts of the supertypic groups described in man.

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“…Responder cells were usually from one dog, but cells from a different dog were occasionally used when the assay was repeated. All dogs differed in their DLA phenotype by at least one haplotype, and responder and stimulator cells were always completely mismatched as determined by alloantisera (19) and canine homozygous typing cells (20). MLC results (mean cpm of triplicate cultures) were expressed as a percentage of respective negative controls (medium with or without rabbit complement or MAb 9.6) according to the formula: where AA was the median cpm of autologous reactions and AB was the median cpm of allogeneic reactions.…”

Section: Monoclonalmentioning

confidence: 99%

Comparative Reactivity of Anti-Ia Monoclonal Antibodies in Man and Laboratory Animals

Severns²,

Pesando³

1988

Hybridoma

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Sixteen murine monoclonal antibodies (MAb), reactive with HLA-DR, DR + DP or DR + DQ, were tested, using indirect immunocytofluorescence, for their reactivity with peripheral blood mononuclear cells (PBMC) from the dog, cat, guinea pig, sheep, rabbit and rat. In addition, the MAb were evaluated for inhibitory activity in the canine mixed lymphocyte culture (MLC). Fourteen of 16 MAb reacted with canine PBMC. There was a greater tendency for DR + DP reactive MAb to inhibit canine MLC and subsequently react with PBMC of the guinea pig, sheep, and, cat. MAb failing to block the canine MLC were generally nonreactive with guinea pig PBMC (7 of 9 nonreactive) suggesting the guinea pig may be a useful model to study the functional relevance of specific Ia molecules. One MAb, H81.98.21 (reactive with HLA-DR) blocked canine MLC and reacted with PBMC from all species tested suggesting the determinant it recognized to be very well conserved in nature.

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Joint Report of the Third International Workshop on Canine Immunogenetics

Cited by 41 publications

References 0 publications

Minor Histocompatibility Antigens on Canine Hemopoietic Progenitor Cells

Minor Histocompatibility Antigens on Canine Hemopoietic Progenitor Cells

Allelic variation in theDR subregion of the canine major histocompatibility complex

Comparative Reactivity of Anti-Ia Monoclonal Antibodies in Man and Laboratory Animals

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