Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa

Abstract

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

Transcriptome and Metabonomics Combined Analysis Revealed the Defense Mechanism Involved in Hydrogen-Rich Water-Regulated Cold Stress Response of Tetrastigma hemsleyanum

“…But in our study, HRW treatment inhibited the accumulation of coniferyl alcohol and eriodictyol, which may be due to the redirection of metabolic fluxes in branches of the phenylpropane pathway in response to cold stress. Zhuang et al (2021) indicated that the coniferyl alcohol content of H. vernicosa increased, which indicated that the antioxidant system of H. vernicosa was activated and coniferyl alcohol was synthesized to improve its cold tolerance. This result is contrary to our findings, so we speculate that differences in plant species and abiotic stress types may be responsible for this contradiction.…”

Transcriptomic and metabolomic insights on the molecular mechanisms of flower buds in responses to cold stress in two Camellia oleifera cultivars

“…Sinapyl alcohol is among the precursors of lignin, which is associated with water and solute transport and cell wall rigidity, and contributes to abiotic stress resistance (Lee et al, 2021). Hosta ventricosa enhances its cold resistance by adjusting the ratio of sinapyl alcohol to coniferyl alcohol, thereby altering the morphological structure of the cell wall (Zhuang et al, 2021).…”

Crosstalk between Ca2+ and Other Regulators Assists Plants in Responding to Abiotic Stress

“…In contrast to CaM and CBL, which have to couple with Ca 2+ to change their conformation and be activated, CDPKs, which are constitutively activated and directly phosphorylated, transduce calcium signals by interacting with the site of the calcium receptor or forming a peptide chain [ 62 ]. CDPKs are involved in the intermediate process instead of participating in the initial response to low temperature in rice.…”