Bus lanes play a crucial role in urban areas as their primary objective is to increase public transport efficiency and help traffic and public transit systems flow more smoothly. This study starts with traffic and climate monitoring to verify asphalt bus lanes in Rome, Italy, according to the Italian Pavement Design Catalogue published in 1995. KENLAYER software calculated the stress-strain conditions under real traffic loads (i.e., hourly passages of urban buses, considering their axle load and seat occupancy rate), typical subgrade bearing capacity (i.e., resilient modulus equal to 90 MPa), current climate conditions, and road material properties. Then, the Mechanistic-Empirical Pavement Design Guide (MEPDG) was used to verify the response of the pavement structure. The fatigue verification of bound materials resulted in damage values much lower than 1 at the end of the 20-year service life (i.e., 0.12 with the Asphalt Institute and 0.31 with the Marchionna law, respectively) and highlights that the Italian catalogue’s sheets are overdesigned. On the other hand, the rutting verification according to MEPDG is not satisfied after an 11-year service life (i.e., the total rutting is equal to 1.50 cm), forcing frequent and expensive maintenance of wearing and binder courses. Therefore, the results confirm the validity of the Italian catalogue for fatigue service life and suggest the need for high-performance asphalt to prevent early rutting due to bus traffic increasing by load and frequency in previous decades.