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Background: Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods: Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 10 8 propagules/ml and the bacterium inocula were adjusted at 10 7-10 9 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results: Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The biocontrol agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion: The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.
Background: Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods: Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 10 8 propagules/ml and the bacterium inocula were adjusted at 10 7-10 9 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results: Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The biocontrol agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion: The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.
ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ В АГРОЦЕНОЗЕ КОРМОВЫХ БОБОВ В БЕЛАРУСИАннотация. Производство отечественного высокобелкового корма является ключевой проблемой в животноводческой отрасли Республики Беларусь, решить которую можно путем расширения посевных площадей под зернобобовые культуры, в частности кормовые бобы. Особую актуальность приобретают исследования по оценке эффективности биологических препаратов в посевах культуры против болезней как возможность получения экологически чистой продукции с минимальным негативным воздействием на окружающую среду. Предпосевная обработка семян биологическим препаратом Фунгилекс, Ж (Trichoderma sp. D-11) -(8,0-10,0 л/т) позволяет, наравне с химическим протравителем Скарлет, МЭ (имазалил, 100 г/л + тебуконазол, 40 г/л) -(0,4 л/т), снизить поражение посевов культуры альтернариозом на 73,8-78,8 %, фузариозом -на 53,3-56,7 %, что обеспечивает сохранение 2,8-3,0 ц/га зерна культуры относительно контроля. Внесение в почву перед посевом кормовых бобов инокулянта микробиологического Ресойлер, Ж (Trichoderma sp. L-3 и Trichoderma sp. L-6) -(6,0-8,0 л/га) способствует нарастанию вегетативной массы на 3,2-3,5 %, снижению развития фузариоза на 69,1-70,6 % и повышению семенной продуктивности на 3,1-3,6 ц/га относительно контроля. Комплексное применение биопрепаратов Фунгилекс, Ж (8,0 и 10,0 л/т) и Ресойлер, Ж (6,0 и 8,0 л/га) способствует нарастанию надземной части растений на 3,5-3,8 %, снижению развития альтернариоза на 88,7-90,1 %, фузариоза -на 76,5-79,4 %. Это обеспечивает статистически достоверное сохранение 5,1-5,6 ц/га зерна культуры относительно контроля и получение максимального чистого дохода 231,5-249,5 руб/га, что на 32,6-50,6 руб/га выше по сравнению с протравителем Скарлет, МЭ (0,4 л/т). Важно отметить, что защитное действие как биологических препаратов, так и химического протравителя отмечалось до фазы полного стеблевания культуры (ВВСН 35). Использование биологических препаратов позволяет снизить пестицидную нагрузку на культуру, защитить растения от болезней и обеспечить получение экологически чистой продукции. Результаты исследований могут широко применяться в сельском хозяйстве.
IntroductionA two-year research trial was conducted to evaluate progression of common bean (Phaseolus vulgaris) growth, Rhizoctonia root rot and weed development in association with productivity when treated with different cultivars, planting dates and weed control methods in north-western part of Iran.MethodsTo determine the best descriptors, six standard curves were examined to model development of bean dry matter, Rhizoctonia root rot incidence, and weed density during two growing seasons across 256 field plots. Exponential and linear-by-linear models were fitted to bean-disease-weed progression data, and then model parameters representing over-season progress curve elements were used in multivariate regression analyses to estimate bean production. Results and discussionFurthermore, using herbicides (Imazethapyr and Trifluralin) restricted weed density by 28% in early (mid-spring) and 42% in late (late spring to early summer) plantings. Late plantings of two bean cultivars decreased disease progress up to 36% for herbicide use, hand-weeding and control. Although bean dry matter, pod and seed production for herbicide use and hand-weeding treatments were 6-17% greater than control, late planting improved productivity in control by 10-24%. Findings suggested that late planting of bean improved efficiency of herbicides to control weeds. Late planting also restricted Rhizoctonia root rot progress and thus, improved bean yield. There were significant correlations between bean-disease-weed development descriptors. According to principal component analysis, bean-disease-productivity-weed variables accounted for 80% of total data variance. Such information extends our understanding of bean-disease-weed progress in interaction with planting date to develop more effective and sustainable integrated Rhizoctonia management programs.
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