JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential

Li, Hongliang; Chen, Dandan; Li, Xiaohong; Zhang, Haiwei; Lü, Jun-Hua; Ren, Xian-Da; Wang, Cunchuan

doi:10.3748/wjg.v8.i2.217

Cited by 13 publications

(3 citation statements)

References 56 publications

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“…The descent of rhodamine can reflect the collapse of ΔΨm [16] . In order to determine the effect of PE on ΔΨm, the uptake of rhodamine was detected by flow cytometry.…”

Section: Effect Of Pe On δψM Of Hepg2 Cellsmentioning

confidence: 99%

Exogenous phosphatidylethanolamine induces apoptosis of human hepatoma HepG2 cells via the bcl-2/bax pathway

Yao¹,

Huang²,

Li³

et al. 2009

WJG

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AIM:To investigate the signaling pathways implicated in phosphatidylethanolamine (PE)-induced apoptosis of human hepatoma HepG2 cells. METHODS:Inhibitory effects of PE on human h e p a t o m a H e p G 2 c e l l s w e r e d e t e c t e d b y 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle, apoptosis and mitochondrial transmembrane potential (ΔΨm) were analyzed by flow cytometry. Immunocytochemical assay and Western blotting were used to examine Bcl-2, Bax and caspase-3 protein levels in HepG2 cells treated with PE. RESULTS:PE inhibited the growth of HepG2 cells in a dose-and time-dependent manner. It did not affect the cell cycle, but induced apoptosis. PE significantly decreased ΔΨm at 0.25, 0.5 and 1 mmol/L, respectively, suggesting that PE induces cell apoptosis by decreasing the mitochondrial transmembrane potential. The Bcl-2 expression level induced by different concentrations of PE was lower than that in control groups. However, the Bax expression level induced by PE was higher than that in the control group. Meanwhile, PE increased the caspase-3 expression in a dose-and time-dependent manner. CONCLUSION:Exogenous PE induces apoptosis of human hepatoma HepG2 cells via the bcl-2/bax pathway.

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“…The descent of rhodamine can reflect the collapse of ΔΨm [16] . In order to determine the effect of PE on ΔΨm, the uptake of rhodamine was detected by flow cytometry.…”

Section: Effect Of Pe On δψM Of Hepg2 Cellsmentioning

confidence: 99%

Exogenous phosphatidylethanolamine induces apoptosis of human hepatoma HepG2 cells via the bcl-2/bax pathway

Yao¹,

Huang²,

Li³

et al. 2009

WJG

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“…After 24 hours, supernatant of the wells was removed, their cells were washed twice with PBS and resuspended by adding trypsin /EDTA (Gibco, Glasgow, UK). [ 20 21 ] After centrifugation, the pellet cells were resuspended in 1 ml of HPSS salt solution and its volume was increased to 10 ml with 70% ethanol. The suspension was maintained at –20° C till the time of evaluating experiments.…”

Section: Methodsmentioning

confidence: 99%

Nano - drug delivery of apoptosis activator 2 to AGS cells by liposomes conjugated with anti-TROP2 antibody

2012

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Background:Gastric cancer is the second most common causes of cancer related death in the world and is responsible for two third of cancer related death in the developing countries. survival rate surgery is low and radiation therapy and chemotherapy as alternatives ways for treatment of gastric cancer are not very promising. Thus there is an urgent need for introducing novel treatment procedures and promising new anti-canceric drugs.Aim:In this study we used pre-prepared liposomes and after necessary manipulations ,these modified liposomes were used for delivery of apoptosis activator 2 to gastric adenocarcinoma cell line (AGS).Materials and Methods:we used pre-prepared liposomes and after necessary manipulations, these modified liposomes were used for delivery of apoptosis activator 2 to AGS cells and induced apoptosis was evaluated by related apoptotic DNA ladder, TUNNEL and Cell Death experiments.Results:Evaluation of apoptosis by Apoptotic DNA Ladder in liposome treated and untreated AGS cells by DNA laddering and fragmentation, TUNEL and Cell Death Detection confirmed that treatment of AGS cell lines with apoptosis activator 2 loaded liposomes which targeted cell surface TROP2 antigen in cancer cells significantly increased apoptosis in these cells.Conclusion:Nano drug delivery of apoptosis activator 2 to human gastric adenocarcinoma cell line with liposomes targeted TROP2 antigen is a possible way for smart killing of human gastric adenocarcinoma cells.

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“…Experimental have indicated that pre-exposure of MCF-7 breast cancer cells to paclitaxel followed by 5-FU was preferable [5] . During the last 20 years, accumulating data have shown an anti-proliferative effect of non-steroidal anti-inflammatory drugs (NSAIDs) in a variety of malignant cell lines [6][7][8][9][10][11][12] . PGs and their synthesizing enzyme COX-2 was suggested to be involved in carcinogenesis [13] .…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of nimesulide and 5-fluorouracil on tumor growth and apoptosis in the implanted hepatoma in mice

Xiao-hong

Li²,

Cai³

et al. 2003

WJG

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AIM:To compare the effect of nimesulide or/and 5-fluorouracil (5-FU) on tumor growth inhibition and apoptosis in mice with the implanted hepatoma and to observe their possible interactions. METHODS:The inhibitory effects on tumor growth was evaluated by inhibition rate. Apoptosis was assessed by the ultrastructural, flow cytometry features and the DNA ladder demonstrated by agarose gel electrophoresis. PGE 2 level was determined by radioimmunoassay. Expression levels of c-jun, c-fos and p53 were evaluated by western blotting. RESULTS:Nimesulide or 5-FU alone inhibited the growth of hepatoma, while a synergistic effect was observed for a combined use of both. More pronounced morphologic changes for tumor cell apoptosis and the DNA ladder were found for the latter treatment. Expression levels of c-jun and p53 were found to be elevated for the tumors from mice treated with nimesulide and 5-FU comparing to those with either of them, but a reduced PGE 2 level was observed only for the treatment with nimesulide. No change was detected on c-fos expression. CONCLUSION:Nimesulide and 5-FU appear to have synergistic effects for the growth inhibition and apoptosis induction. Both were found to be overexpressed in p53 and c-jun proteins, rather than that of c-fos, associations with the resulted apoptosis.

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JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential

Cited by 13 publications

References 56 publications

Exogenous phosphatidylethanolamine induces apoptosis of human hepatoma HepG2 cells via the bcl-2/bax pathway

Exogenous phosphatidylethanolamine induces apoptosis of human hepatoma HepG2 cells via the bcl-2/bax pathway

Nano - drug delivery of apoptosis activator 2 to AGS cells by liposomes conjugated with anti-TROP2 antibody

Synergistic effects of nimesulide and 5-fluorouracil on tumor growth and apoptosis in the implanted hepatoma in mice

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