In Chapter 3, reticular fatty acid (FA) flow and ruminal BH of C18:3n-3 is reported using the reticular sampling technique (Cr-EDTA and Yb-acetate as digesta flow markers) in the lactating cows fed the SAIN and CON diet in Chapter 2. The reticular flows of DM, OM and N were not affected by dietary treatment. However, NDF flow was higher (1.87 vs. 1.40 kg/d) where the cows were fed the SAIN diet. A higher mono-unsaturated FA flow was caused by the higher trans-9-C18:1 and cis-9-C18:1 flow for the SAIN compared to the CON fed cows. The flows of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 were higher in the SAIN diet fed cows, but total poly-unsaturated FA flow was not affected by the different diet treatments. The SAIN diet fed cows had a significant lower ruminal BH of cis-9-C18:1 and C18:3n-3, compared to the CON fed cows and tended to a lower ruminal BH in case of cis-9,cis-12-C18:2. These results show that inclusion of sainfoin silage at the expense of grass silage in dairy cow rations reduces ruminal BH of dietary cis-9-C18:1 and C18:3n-3. The effects of replacing grass silage by sainfoin silage in a TMR on milk production and FA in milk fat of the dairy cows in Chapter 2 is reported in Chapter 4. Milk yield reported in Chapter 4 was highest for the SAIN diet with every kg of OM digested of the SAIN diet resulting, on average, in 0.2 kg more milk production. The SAIN diet fed cows had a higher C18:3n-3 and cis-9,cis-12-C18:2 proportion in milk fat compared to the CON diet fed cows. A higher proportion of total trans-C18:1 was found in the cows fed the SAIN diet. There were no differences in proportion of total saturated and unsaturated FA in milk fat between the two diets. Our results showed that replacing grass silage by sainfoin silage improved milk yield and milk FA profile of dairy cows. Effects of the structural properties of CT, i.e. average polymer size (or mean degree of polymerization, mDP); percentage of cis flavan-3-ols (%cis) and percentage of prodelphinidins (%PD) in CT extracts on CH 4 production and fermentation characteristics of rumen fluid using an in vitro gas production technique was investigated in Chapter 5. Extracts of CT from eight plants; black currant leaves, goat willow leaves, goat willow twigs, pine bark, red currant leaves, sainfoin plants, weeping willow catkins and white clover flowers were extracted, in order to obtain CT with a wide range in mDP, %PD and %cis. All CT extracts reduced CH 4 concentration, decreased the maximum rate of fermentation for CH 4 production and rate of substrate degradation. The correlation between CT structure on the one hand and CH 4 production and fermentation characteristics on the other hand showed that the %PD within CT had the largest effect on fermentation characteristics, followed by mDP and %cis. Chapter 6 reports results of an in vitro study to investigate the effects of the structural properties CT (mDP, %cis and %PD) on rumen fermentation and BH end-products. The total volatile FA (VFA), ammonia concentration and the proportion of bra...