Twenty-one new species of jumping spiders from five provinces of South China are described: Cheliceroides jinxinisp. nov. (♂), Dendroicius qiongsp. nov. (♂♀), Icius deergongsp. nov. (♂♀), Irura qiuhangisp. nov. (♂♀), I. yarlungzangbosp. nov. (♂♀), Mintonia shiwandashansp. nov. (♂), Myrmarachne kuansp. nov. (♂♀), Nandicius xiefengisp. nov. (♂♀), Pancorius medogsp. nov. (♀), P. yingjiangsp. nov. (♂♀), Piranthus maddisonisp. nov. (♂♀), Simaetha hainansp. nov. (♂♀), Stertinius lhobasp. nov. (♂♀), Synagelides kongmingisp. nov. (♂♀), S. xuandeisp. nov. (♂♀), S. yunchangisp. nov. (♂♀), S. yideisp. nov. (♂), S. zilongisp. nov. (♂♀), Yaginumaella daweishansp. nov. (♂♀), Y. moinbasp. nov. (♂♀), and Y. pingbiansp. nov. (♂♀). Nepalicius Prószyński, 2016, syn. nov. is proposed as a junior synonym of Okinawicius Prószyński, 2016. Three new combinations are proposed: O. nepalicus (Andreeva, Hęciak & Prószyński, 1984), comb. nov. and O. seychellensis (Wanless, 1984), comb. nov. transferred from Nepalicius, and O. daoxianensis (Peng, Gong & Kim, 2000), comb. nov. transferred from Philaeus Thorell, 1869. The unknown females of O. nepalicus, Padillothorax exilis (Cao & Li, 2016) and Siler hanoicus Prószyński, 1985 are described for the first time. Distribution maps of the studied specimens are also provided.