Jungle Yellow Fever, Rio de Janeiro

Filippis, Ana María Bispo de; Schatzmayr, Hermann G.; Nicolai, Cecília; Baran, Mary; Miagostovich, Marize Pereira; Sequeira, Patrícia Carvalho de; Nogueira, Rita Maria Ribeiro

doi:10.3201/eid0703.017331

Cited by 10 publications

(2 citation statements)

References 3 publications

(1 reference statement)

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“…Sporadic cases of yellow fever still occur within endemic areas, affecting unvaccinated individuals that get close to or enter into tropical forests (3,(7)(8)(9)(10). An annual estimate of 200,000 cases and 30,000 deaths makes it a significant cause of morbidity and mortality worldwide (1)(2)(3).…”

mentioning

confidence: 99%

Desarrollo de un método de transcripción reversa seguida de reacción en cadena de la polimerasa para la detección del virus de la fiebre amarilla

et al. 2012

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Introduction. Yellow fever is considered a re-emerging disease and is endemic in tropical regions of Africa and South America. At present, there are no standardized or commercialized kits available for yellow fever virus detection. Therefore, diagnosis must be made by time-consuming routine techniques, and sometimes, the virus or its proteins are not detected. Furthermore, co-circulation with other flaviviruses, including dengue virus, increases the difficulty of diagnosis. Objective. To develop a specific reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR-based assay to improve the detection and diagnosis of yellow fever virus using both serum and fresh tissue samples. Materials and methods. RT-PCR primers were designed to amplify a short fragment of all yellow fever virus genotypes reported. A second set of primers was used in a nested PCR to increase sensitivity. Thirty-three clinical samples were tested with the standardized reaction. Results. The expected amplicon was obtained in 25 out of 33 samples analyzed using this approach, and 2 more samples tested positive after a subsequent nested PCR approach. Conclusion. This improved technique not only ensures the specific detection of a wide range of yellow fever virus genotypes but also may increase the sensitivity of detection by introducing a second round of amplification, allowing a rapid differential diagnosis between dengue and yellow fever infection, which is required for effective surveillance and opportune epidemiologic measures.

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confidence: 99%

Desarrollo de un método de transcripción reversa seguida de reacción en cadena de la polimerasa para la detección del virus de la fiebre amarilla

et al. 2012

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“…In Brazil until 1942, when the last three cases of urban YF were reported [Calheiros, 1988], the sylvatic and urban modes of YF transmission coexisted. Nowadays, despite the existence of a potent vaccine, cases of jungle YF still occur every year [Filippis et al, 2001;Ministry of Health, 2001], mainly in states from the North and Midwest regions and some sporadic cases in the Southeast region. For Brazil as a whole from 1980 to 2001, 578 cases of jungle YF were reported, with 307 deaths (53.1% lethality rate).…”

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confidence: 99%

Outbreak of jaundice and hemorrhagic fever in the Southeast of Brazil in 2001: Detection and molecular characterization of yellow fever virus

Filippis

Nogueira

Schatzmayr

et al. 2002

Journal of Medical Virology

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Between January and March 2001, an outbreak of jaundice and hemorrhagic fever occurred in the state of Minas Gerais, Southeast region of Brazil, in which a mortality rate of 53% was reported. Seroconversion, virus isolation, histopathological and immunohistochemical findings, and reverse transcription-polymerase chain reaction (RT-PCR) identified yellow fever virus (YFV) as the etiological agent responsible for the outbreak. Partial nucleotide sequence analysis from a fragment of the YFV genome spanning parts of nonstructural (NS) 5 gene and 3' noncoding region (3' UTR) showed that the YFV involved in this outbreak belongs to South American genotype I and differs from the Brazilian virus identified in 1996.

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