Justification of Traditional Uses of Asparagus Racemosus (Shatavari) - A Miracle Herb

Abstract

Objective: The ethanolic plant extracts of Asparagus racemosus and their partitionates were assessed for thrombolytic, membrane stabilizing, antimicrobial and antioxidant activity in vitro. Methods: The collected plant was dried and grinded. The coarse powder was shocked in 2000 mL of 90% methanol for several days then filtrated. At 40°C the volume of crude ethanolic extract (CEE) was reduced by a vacuum rotary evaporator followed by concentrated aqueous ethanol extractive was fractionated into petroleum ether (PSF), carbon tetrachloride (CTSF), chloroform soluble fraction (CSF) and aqueous (AQSF) soluble fractions. Results: In thrombolytic assay among all the extractives, carbon tetrachloride soluble fraction (CTSF) showed the highest clot lysis (41.48 ± 0.43%) activity as compared with standard streptokinase (65.16 ± 0.16%). With respect to the membrane stabilizing activity, chloroform soluble fraction (CSF) profoundly inhibited the lysis of erythrocytes (80.86 ± 0.36%) induced by osmosis, whereas reference standard acetyl salicylic acid (ASA) resulted in (88.99 ± 0.39%) inhibition of lysis. On the other hand, CTSF showed slightly higher level of protection against heat-induced hemolysis (85.68 ± 0.75%) as same as with ASA, (85.68 ± 0.75%)). In antimicrobial investigation, crude ethanol extract (CEE) and their partitioning fractions exhibited moderate inhibition of growth of some examined bacteria. The total phenolic content was found maximum (930.94 mg of GAE/gm) in CEE of A. racemosus. The antioxidant potentials in term of DPPH free radical scavenging assay showed EC50 values 2.62 μg/mL by aqueous soluble fraction (AQSF) in comparison to ascorbic acid 2.48 μg/mL as a reference standard. Conclusions: This study was conducted to validate the A. racemosus plant as thrombolytic, membrane stabilizing agent, antimicrobial and antioxidant.

Management of inflammatory disorders: A brief review of selected ethnomedicinal plants

“…racemosus is an extensively used herb in Ayurvedic medicine system. This spinous under shrub commonly grows at low altitudes of tropical and subtropical countries [49]. In in vivo experimental models, this species has shown significant reduction of rat paw edema induced by carrageenan and serotonin [50,51].…”

“…In in vivo experimental models, this species has shown significant reduction of rat paw edema induced by carrageenan and serotonin [50,51]. Whereas in vitro assays demonstrated that this plant has promising RBC membrane stabilization activity [49,52,53]. A. racemosus extract demonstrated anti-inflammatory activity by reducing pathology of inflammatory bowel disease (IBD) in oxazolone induced zebra fish IBD (inflammatory bowel disease) model [54].…”

Potentiality of Crataeva nurvala bark in the management of thrombosis and membrane stabilization in the rural area, Bangladesh

“…The clear supernatant was decanted, and remaining RBC cells were undertaken for membrane stabilizing activity. Finally, cellular components (0.5 ml erythrocyte suspension, 5 ml hypotonic solution, and 50 mM NaCl in 10 mM sodium phosphate buffered saline) were well mixed for in vitro membrane stabilizing assay [29].…”

Asparagus racemosus: Many problems, one solution on its phytochemical and pharmacological potential

“…In Ayurveda, this unique herb is called as "Queen of herbs" because it promotes love and devotion (Pandey et al, 2021) [3] . Asparagus racemosus is one unique drugs in Ayurveda and effective in treating madhur vipakam, seet -veeryam, somrogam, chronic fever and internal heat (Karim S. et al, 2017) [4] . Charak Samhita and Ashtang Hridyam, the two main texts on ayurveda medicines uses Asparagus racemosus for the formula to treat women's health disorder.…”