Juvenile hormone induction of glutathione S‐transferase activity in the larval fat body of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Abstract

The effect of pyriproxyfen, a juvenile hormone analog (JHA), on the pupation of S. litura was examined. A topical application of 100 µg JHA/larva on the newly ecdysed (0-day) sixth instar larvae resulted in more than 80% pupation, while most of the 1-or 2-day-old larvae similarly treated developed into supernumerary larvae. Glutathione S-transferse (GST) activity in the fat body of 0-day-old sixth instar larvae was significantly induced within 12 h of JHA (100 µg/larva) treatment. In contrast, no such induction was found when 1-and 2-day-old sixth instar larvae were similarly treated. This induction phenomenon was also observed when 0-day-old sixth instar larvae were treated with the natural JH III. The suppressive effects of α-amanitin and cycloheximide suggest that JHA induction of GST activity in these cutworm larvae presumably occurred at the gene transcription level. Arch. Insect Biochem. Physiol. 68:232-240, 2008.

Regulation of melanization by glutathione in the moth Pseudoplusia includens

“…Collectively, these findings may reflect actual differences in GSH concentrations among hemocyte types, but could also indicate differences between hemocyte types in glutathione S-transferase (GST) activity or expression of different GST subtypes unable to react with MCB. Several studies indicate that Lepidoptera and other insects express GSTs, but GST activity in hemocytes is unknown (Weinhold et al, 1990;Wu and Lu, 2008). The levels of GSH released in our primary cultures are also lower than found in newly collected plasma, but we think this likely reflects culture in a minimal saline medium.…”

Salivary and Intestinal Transcriptomes Reveal Differential Gene Expression in Starving, Fed and Trypanosoma cruzi-Infected Rhodnius neglectus

“…On the other hand, perhaps as a defense mechanism of R. neglectus when trying to keep the environment more oxidizable, increased transcriptional expression of a yet unknown T3 peptidase occurs (Figure 9B). T3 peptidases are fundamental in the regulation of metamorphosis (Wu and Lu, 2008), insecticide metabolism (Pickett and Lu, 1989), and in the degradation of glutathione, an important antioxidant for the cell and for Tc itself (Comini et al, 2005;Krauth-Siegel and Comini, 2008).…”

Impairment of Development and Reproductivity of the Egyptian cotton leafworm Spodoptera littoralis Boisduval (Noctuidae: Lepidoptera) by Cycloheximide.

“…In insects, also, cycloheximide exhibited an anti-gonadotropic action in insects, such as the tobacco caterpillar Spodoptera litura (Sridevi and Ray, 1988) and the mealworm beetle Tenebrio molitor (Soltani-Mazouni and Soltani, 1995). In addition, cycloheximide was recorded as a suppressive agent on the enzyme activities in insects, such as the detoxifying enzyme Glutathione S-transferse in the common cutworm Spodoptera litura (Wu and Lu, 2008); phenoloxidase in Spodoptera exigua (Shrestha and Kim, 2007) and transhydrogenase in the midgut mitochondria in M. sexta 5 th instar larvae (Vandock et al, 2010). The present study was carried out aiming at the assessment of disruptive effects of cycloheximide on survival, growth, development, metamorphosis and reproductive potential of S. littoralis.…”