Juvenile hormone regulates the reproductive diapause through <scp>Methoprene‐tolerant</scp> gene in <scp><i>Galeruca daurica</i></scp>

Ma, Hong-Yue; Li, Y.-Y.; Li, L.; Tan, Yao; Pang, Bao-Ping

doi:10.1111/imb.12710

Cited by 20 publications

(18 citation statements)

References 45 publications

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“…In this study, we found that depleting FoxO delayed the entry of G. daurica adults into diapause. The same result has been obtained after methoprene injections (Ma et al ., 2021a). These results indicate that FoxO plays an important role in regulating reproductive diapause in G. daurica .…”

Section: Discussionmentioning

confidence: 99%

“…The G. daurica larvae were collected in May, 2021 from the Xianghuang Banner grasslands of Inner Mongolia, China, and they were reared on Allium mongolicum under natural conditions as described by Ma et al . (2021a). For the expression profiling, female adult samples were collected at 1, 3, 7, 10, 15, 25, 40, 60, 80, and 100 d posteclosion and the samples were maintained at −80 °C until RNA was extracted.…”

Section: Methodsmentioning

confidence: 99%

“…Ma et al . (2021a, 2021b) have demonstrated that JH‐signaling plays a crucial role in regulating reproductive diapause in G. daurica . In our previous study, we identified 222 microRNAs at different diapausal stages in G. daurica adults, and we found that miR‐2765‐3p was mostly downregulated, up to over 32‐fold, after adults entered into diapause (Duan et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

Duan

Wang

et al. 2022

Insect Science

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The forkhead box O (FoxO), as a conserved transcription factor, plays an indispensable role in regulating insect diapause. However, how FoxO is regulated to control diapause in insects remains unknown. In this study, we discovered functional binding sites for miR-2765-3p in the 3 untranslated region of FoxO in Galeruca daurica. The luciferase reporter assay showed that miR-2765-3p targeted FoxO and suppressed its expression. The expression profiles of miR-2765-3p and FoxO displayed opposite patterns during the female developmental process. Overexpression of miR-2765-3p by the injection of the miR-2765-3p agomir into adult females reduced FoxO expression, leading to the suppression of lipid accumulation, promotion of ovarian development, and inhibition of reproductive diapause. This is similar to the phenotype that results from the depletion of FoxO by injecting dsFoxO into adult females. In addition, the repression of miR-2765-3p by injecting the miR-2765-3p antagomir increased the FoxO transcript level, leading to the stimulation of lipid accumulation, depression of ovarian development, and induction of reproductive diapause. A hormone injection assay showed that the juvenile hormone (JH) agonist (methoprene) upregulated miR-2765-3p and downregulated FoxO. Notably, injecting methoprene rescued ovarian development defects associated with miR-2765-3p inhibition. These findings indicate that the JH/miR-2765-3p/FoxO axis plays a vital role in the regulation of reproductive diapause in G. daurica.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

Duan

Wang

et al. 2022

Insect Science

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“…Methoprene-tolerant (Met) is a universal JH receptor. Silencing GdMet inhibits the expression of JHE and Vg , causing reproductive diapause of Galeruca daurica ( Ma et al, 2021 ). Adenylyl cyclase (AC) plays a role in cell signaling processes.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål)

Zhang

et al. 2022

Front. Physiol.

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SLC26A10 is a member of the SLC26 gene family, but its role in insects is still unclear. We cloned the SLC26A10 gene of Nilaparvata lugens (NlSLC26A10) and found NlSLC26A10 contained 11 transmembrane regions and a STAS domain. Expression pattern analysis showed NlSLC26A10 expression was more upregulated in adults than in nymphs, highest in the ovary. After injection of double-stranded RNA (dsRNA) of NlSLC26A10, the mRNA level of NlSLC26A10 significantly decreased and, consequently, the ovarian development of adult females was hindered; the amount and the hatchability of eggs and yeast-like symbionts in mature oocytes decreased. Further study showed that NlSLC26A10 might result in decreased juvenile hormone level and vitellogenin expression. These results indicate that NlSLC26A10 plays an essential role in the reproduction of N. lugens.

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“…Galeruca daurica (Coleoptera: Chrysomelidae), a serious grassland pest since its sudden outbreaks on the Inner Mongolia grasslands in 2009 ( Zhou et al, 2016 , Zhou et al, 2019 ), has continually spread and caused great losses to pasture on the Inner Mongolia grasslands ( Ma et al, 2019 , Ma et al, 2021 ), and levels of damage has increased year by year ( Duan et al, 2022 ). The application of traditional chemical insecticides to control outbreaking pests in desert steppes has the advantages of a good insecticidal effect with rapid results, especially organophosphorus, pyrethroids, chloronicotinyl insecticide ( Dong et al, 2016 ; Gao et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)

Ren

Zhang²,

Ni³

et al. 2022

Front. Physiol.

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In recent years, the leaf beetle Galeruca daurica has broken out in the northern grasslands of Inner Mongolia, its management still mainly depends on chemical control using traditional insecticides or with novel action. The study was aim to identify mutation locus associated with resistance to diamide insecticides in field population of G. daurica, to provide a reference for rational selection of insecticides and to avoid the rapid resistance development to diamide insecticides. We cloned the full length of the ryanodine receptor gene of G. daurica (GdRyR), constructed 3D model and transmembrane regions by homologous modeling based on deduced amino acid sequence. Two potential mutation loci (Gly4911Glu and Ile4754Met) and allelic mutation frequencies were detected in individuals of G. daurica. In addition, their binding patterns to two diamide insecticides (chlorantraniliprole, cyantraniliprole) were analyzed separately using a molecular docking method. The full-length cDNA sequence of GdRyR (GenBank accession number: OP828593) was obtained by splicing and assembling, which is 15,399 bp in length and encodes 5,133 amino acids. The amino acid similarity of GdRyR with that of other Coleopteran insects were 86.70%–91.33%, which possessed the typical structural characteristics. An individual resistance allelic mutation frequency test on fifty field leaf beetles has identified 12% and 32% heterozygous individuals at two potential mutation loci Gly4911Glu and Ile4754Met, respectively. The affinity of the I4754M mutant model of GdRyR for chlorantraniliprole and cyantraniliprole was not significantly different from that of the wild type, and all had non-covalent interactions such as hydrogen bonding, hydrophobic interactions and π-cation interactions. However, the G4911E mutant model showed reduced affinity and reduced mode of action with two diamide insecticides, thus affecting the binding stability of the ryanodine receptor to the diamide insecticides. In conclusion, the G4911E mutation in GdRyR may be a potential mechanism for the development of resistance to diamide insecticides on G. daurica and should be a key concern for resistance risk assessment and reasonable applications of diamide insecticides for control in future. Moreover, this study could provide a reference for ryanodine receptor structure-based insecticides design.

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Juvenile hormone regulates the reproductive diapause through Methoprene‐tolerant gene in Galeruca daurica

Cited by 20 publications

References 45 publications

MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

MicroRNA miR‐2765‐3p regulates reproductive diapause by targeting FoxO in Galeruca daurica

Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål)

Detection of ryanodine receptor G4911E and I4754M mutation sites and analysis of binding modes of diamide insecticides with RyR on Galeruca daurica (Coleoptera: Chrysomelidae)

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