K-Nutrition, Growth Bud Formation, and Amine and Hydroxycinnamic Acid Amide Contents in Leaf Explants of <i>Nicotiana tabacum</i> Variety Xanthi n.c. Cultivated <i>in Vitro</i>

Klinguer, Serge; Martin-Tanguy, J.; Martı́n, Carmen

doi:10.1104/pp.82.2.561

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…The differentiation of buds is always accompanied by significant changes in putrescine and hydroxycinnamoyl putrescine levels (14). Increasing levels were found during the first week in culture when cell multiplication is rapid.…”

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confidence: 83%

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Effects of the Suicide Inhibitors of Arginine and Ornithine Decarboxylase Activities on Organogenesis, Growth, Free Polyamine and Hydroxycinnamoyl Putrescine Levels in Leaf Explants of Nicotiana Xanthi n.c. Cultivated in Vitro in a Medium Producing Callus Formation

Burtin¹,

Martin-Tanguy²,

Paynot³

et al. 1989

Plant Physiol.

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We studied the effects of DL-a-difluoromethylarginine (DFMA) and DL-a-difluoromethylomithine (DFMO), specific, irreversible inhibitors of arginine decarboxylase (ADC) and omithine decarboxylase (ODC), respectively, on organogenesis growth and titers of free polyamines and conjugated putrescines (hydroxycinnamoyl putrescines) in tobacco (Nicotiana tabacum cv Xanthi n.c.)calli. These results suggest that ADC and ODC regulate putrescine biosynthesis during early and later stages of tobacco callus development, respectively. ADC appears active in biosynthesis of large levels of free amines (agmatine and putrescine) while ODC appears active only in biosynthesis of large levels of putrescine conjugates (hydroxycinnamoyl putrescines). DFMA inhibits the fresh and dry weight increases of tobacco calli, whereas DFMO even promoted the fresh and dry weight increases, thus supporting the view that ADC is important for cell division and callus induction. Inhibition of ODC activity by DFMO resulting in an amide deficiency after 4 weeks of culture facilates the expression of differentiated cell functions. Formation of buds is associated with a significant decrease of hydroxycinnamoyl putrescines.Polyamines and their biosynthetic enzymes may play an important role in many aspects of plant development including growth, differentiation, senescence, and response to stress (1, 10, 26). Increased polyamine synthesis appears necessary for cell proliferation to occur (3). Amides formed between hydroxycinnamic acid and amines are widely distributed in the plant kingdom (17, 18). Accumulation of hydroxycinnamic acid amides correlates with leaf emergence and flowering in several plants (5,17,19). Tobacco stem apices, callus tissues, and cell cultures often contain polyamines conjugated with cinnamic acids (4,5,11,20). Some functional roles of these compounds have been suggested. Caffeoyl putrescine and other amides such as caffeoyl spermidine have been found in large concentrations in reproductive organs oftobacco, and it has been proposed that their formation is functionally linked with reproduction (5). In addition, since tobacco plants infected with tobacco mosaic virus react by increasing levels of polyamine conjugates, and virus multiplication is retarded in their presence, a role for these compounds in virus resistance has been suggested (15, 19). Recent results indicate that hydroxycinnamoyl putrescines, when included in the medium, interfere in vitro with hormones in the control of cell multiplication and differentiation of tobacco leaf explants (16).For in vitro cultures, it appears that the intracellular levels of putrescine and hydroxycinnamoyl putrescines are markedly enhanced when the cultures are induced to proliferate (14). The differentiation of buds is always accompanied by significant changes in putrescine and hydroxycinnamoyl putrescine levels (14). Increasing levels were found during the first week in culture when cell multiplication is rapid. Then levels declined sharply after 3 weeks of culture as the rate of ...

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confidence: 83%

“…For in vitro cultures, it appears that the intracellular levels of putrescine and hydroxycinnamoyl putrescines are markedly enhanced when the cultures are induced to proliferate (14). The differentiation of buds is always accompanied by significant changes in putrescine and hydroxycinnamoyl putrescine levels (14).…”

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confidence: 99%

Effects of the Suicide Inhibitors of Arginine and Ornithine Decarboxylase Activities on Organogenesis, Growth, Free Polyamine and Hydroxycinnamoyl Putrescine Levels in Leaf Explants of Nicotiana Xanthi n.c. Cultivated in Vitro in a Medium Producing Callus Formation

Burtin¹,

Martin-Tanguy²,

Paynot³

et al. 1989

Plant Physiol.

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“…When foliar explants of N. tabacum cv Xanthi n.c. are cultivated in vitro in a medium promoting bud formation (BA medium as described above), growth (increase in fresh weight) and bud formation (time of ermergence, number of buds) are strongly influenced by K-nutrition (13). In K-deficient medium and in high K medium, growth and bud formation are markedly inhibited.…”

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confidence: 99%

Effect of Hormone Treatment on Growth Bud Formation and Free Amine and Hydroxycinnamoyl Putrescine Levels in Leaf Explant of Nicotiana tabacum Cultivated in Vitro

Martin-Tanguy

Martı́n²,

Paynot³

et al. 1988

Plant Physiol.

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Foliar explants of Nicotiana tabacum cv Xanthi n.c. were cultured on four different media: a basal medium, basal medium plus benzyladenine, basal medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and the basal medium containing both hormones. No differentiation or cell division occurred in leaf explants cultured on the basal medium. Addition of benzyladenine caused the formation of buds on the explants, while 2,4-D caused callus formation and proliferation. Likewise, only callus was formed when explants were cultured on medium containing both hormones, but growth was significantly greater than that of callus grown on a medium containing 2,4-D alone. The levels of amines and hydroxycinnamoyl putrescines were determined in the four types of explants. In nongrowing explants, amines (except an aromatic amine, tyramine) and hydroxycinnamoyl putrescines were always at a low level and only small changes in their concentrations were observed. In callus cultures, amine (except an aromatic amine, phenethylamine) and hydroxycinnamoyl putrescine levels were higher than those found in bud cultures. In all the media, transitory accumulation of aromatic amines occurred after a few days of culture. Higher levels of hydroxycinnamoyl putrescines were attained in callus cultures with a slow growth rate (2,4-D alone) than in callus cultures with a fast growth rate (benzyladenine + 2,4-D). The formation of buds was accompanied by significant changes in putrescine and hydroxycinnamoyl putrescine levels. Increasing levels were found during the first 14 days in culture when cell multiplication was rapid, followed by a sharp decline after 20 days in culture as the rate of cell division decreased and differentiation took place. The relationship among amines, hydroxycinnamoyl putrescines, and cell division and bud formation is discussed.

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“…This observation is also supported by the fact that the original pollenembryos from which calluses were obtained were capable of plant regeneration. Accumulation of putrescine seems to occur in response to various mineral stresses, such as potassium (Klinger et al 1986) or magnesium deficiences (Smith 1984) or excess ammonium ion (Le Redulier & Goas 1975). Thus, the accumulation of PAs in PEC callus could be a stress response due to the forced conditions of in vitro culture and/or to an inadequate composition of the culture medium.…”

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confidence: 99%

Variations in endogenous polyamine content of maize calli obtained from zygotic and androgenetic embryos

Boget

Torné

Willadino

et al. 1995

Plant Cell Tiss Organ Cult

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A comparative study of polyamine (putrescine, spermidine and spermine) levels was conducted with maize calli originating from a) immature embryos and b) pollen embryos capable of plant regeneration. The differences observed in the studied parameters of the two kinds of calluses are related to their cellular origin and to their regeneration capacity. Moreover, only the calluses proceeding from immature embryos differentiated into preembryogenic structures, which eventually developed into plants. Although total polyamine levels in pollenderived calluses were significantly higher than those from immature embryos, spermidine and spermine were the predominant polyamines in both culture types. Furthermore, polyamine fractions of these calluses also showed differences. All these phenomena may be related with the differences observed in the callus embryogenic response. These findings may be useful in understanding the implication of polyamines in embryogenetic processes.

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K-Nutrition, Growth Bud Formation, and Amine and Hydroxycinnamic Acid Amide Contents in Leaf Explants of Nicotiana tabacum Variety Xanthi n.c. Cultivated in Vitro

Cited by 11 publications

References 20 publications

Effects of the Suicide Inhibitors of Arginine and Ornithine Decarboxylase Activities on Organogenesis, Growth, Free Polyamine and Hydroxycinnamoyl Putrescine Levels in Leaf Explants of Nicotiana Xanthi n.c. Cultivated in Vitro in a Medium Producing Callus Formation

Effects of the Suicide Inhibitors of Arginine and Ornithine Decarboxylase Activities on Organogenesis, Growth, Free Polyamine and Hydroxycinnamoyl Putrescine Levels in Leaf Explants of Nicotiana Xanthi n.c. Cultivated in Vitro in a Medium Producing Callus Formation

Effect of Hormone Treatment on Growth Bud Formation and Free Amine and Hydroxycinnamoyl Putrescine Levels in Leaf Explant of Nicotiana tabacum Cultivated in Vitro

Variations in endogenous polyamine content of maize calli obtained from zygotic and androgenetic embryos

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