2013
DOI: 10.1038/modpathol.2013.45
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K5/K14-positive cells contribute to salivary gland-like breast tumors with myoepithelial differentiation

Abstract: Salivary gland-like tumors of the breast show a great variety of architectural patterns and cellular differentiations such as glandular, myoepithelial, squamous, and even mesenchymal phenotypes. However, currently little is known about the evolution and cellular differentiation of these tumors. For that reason, we performed an in situ triple immunofluorescence lineage/differentiation tracing (isTILT) and qRT-PCR study of basal (K5/K14), glandular (K7/K8/18), and epidermal-specific squamous (K10) keratins, p63,… Show more

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Cited by 26 publications
(18 citation statements)
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“…With regard to the origin of salivary gland-like breast tumors, including AdCC and adenomyoepithelioma, Boecker et al speculated that CK5/CK14-positive progenitor cells have a potential to differentiate to glandular and myoepithelial lineages and also to generate heterogeneous cell differentiation such as squamous and mesenchymal progenies [20]. It would be interesting to know molecular mechanisms driving reverse expression of CK5/6 in AdCC and both CK5/6 and CK14 in adenomyoepithelioma, since this may reflect differences in tumorigenesis between these closely related mammary neoplasms.…”
Section: Discussionmentioning
confidence: 99%
“…With regard to the origin of salivary gland-like breast tumors, including AdCC and adenomyoepithelioma, Boecker et al speculated that CK5/CK14-positive progenitor cells have a potential to differentiate to glandular and myoepithelial lineages and also to generate heterogeneous cell differentiation such as squamous and mesenchymal progenies [20]. It would be interesting to know molecular mechanisms driving reverse expression of CK5/6 in AdCC and both CK5/6 and CK14 in adenomyoepithelioma, since this may reflect differences in tumorigenesis between these closely related mammary neoplasms.…”
Section: Discussionmentioning
confidence: 99%
“…Boecker and colleagues recently published a study of salivary gland tumors of the breast and histologically similar tumors of the salivary and lacrimal glands [56]. They utilized triple immunofluorescence to trace the lineage of cells within these tumors.…”
Section: Discussionmentioning
confidence: 99%
“…FFPE sections (thickness, 4 μm) were pretreated and stained as described elsewhere . For immunostaining, we used primary antibodies raised against K5 (rabbit monoclonal; Medac Diagnostica, Hamburg, Germany); K5/6, K14 (basal markers), K7, K8, K8/18, K18 (glandular markers), and K10 (epidermal differentiation‐specific antibody used as a surrogate marker for squamous differentiation) (mouse monoclonals; Dako, Hamburg, Germany; Sigma, Hamburg, Germany; and Dianova, Hamburg, Germany); SMA (rabbit polyclonal; Abcam, Cambridge, MA, USA); vimentin (mouse monoclonal; Abcam and Dako); Ki67 (rabbit monoclonal; Thermo Fisher Scientific, Bonn, Germany); p63 (mouse monoclonal, clone 4A4; Dako); oestrogen receptors (rabbit monoclonal, clone SP1; Thermo Fisher Scientific); and HER2 (rabbit polyclonal; Medac Diagnostica).…”
Section: Methodsmentioning
confidence: 99%