K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin

Rutherford, T. R.; Clegg, J. B.; Weatherall, D. J.

doi:10.1038/280164a0

Cited by 502 publications

(245 citation statements)

References 13 publications

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“…The K562 cell line was grown in suspension culture in a modified RPMI 1640 medium containing 10 % heat-inactivated fetal or newborn calf serum in a humidified atmosphere with 5 % COz [7]. Karyotypic analysis showed a single Ph' chromosome in all the metaphase studied.…”

Section: Culturementioning

confidence: 99%

“…In particular, Rutherford et al [6,7,10] and Benz et al [8] have rigorously shown that embryonic hemoglobins (Hb Portland and Hb Gower) as well as small quantities of fetal Hb (HbF) are produced by the K562 cells after exposure to hemin. Thus, this cell line provides a new tool to study erythroid differentiation and the switch between embryonic and fetal hemoglobins.…”

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confidence: 99%

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Hemoglobin Expression in Clones of K562 Cell Line

et al. 1982

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The K562 cell line was cloned by dilution and the pattern of hemoglobin (Hb) production was analyzed in the clones thus obtained. The pattern of hemoglobin synthesis was different from one clone to another. According to the Hb phenotype the clones were classified in three main groups: group I no detectable Hb; group II Hb Portland; group III Hb Portland + Hb Gower I. The addition of hemin induced a better hemoglobinization and a shift in the pattern of Hb production: clones of group I were induced to produce Hb Portland and clones of group II to synthesize Hb Gower I. A constant order in the sequential expression of the different hemoglobins was observed: no Hb→Hb Portland + Hb Gower I. The proportion of the different globin chains varied from one clone to another, but an inverse correlation between the synthesis of Gγ and ∈ chains was observed. The α/non‐α chain ratio was unbalanced in all the clones and the addition of hemin induced only a moderate increase of the synthesis of α chains. Recloning of three primary clones increased the homogeneity of the hemoglobin pattern, in particular after hemin induction.

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Section: Culturementioning

confidence: 99%

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confidence: 99%

Hemoglobin Expression in Clones of K562 Cell Line

et al. 1982

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“…K-562 is a chronic myelogenous leukemia cell line that has a bipotential differentiation capacity: the cells have features of erythroleukemia (8,9), but treatment with tumor promoters induces them to differentiate into the megakaryoblastoid direction (10)(11)(12). We were interested in tyrosine kinases expressed in these cells because of their potential role in platelet/erythrocyte-specitic tyrosine phosphorylation.…”

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confidence: 99%

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Partanen

Mäkelä

Alitalo

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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Tyrosine phosphorylation is important in the transmission of growth and differentiation signals; known tyrosine kinases include several oncoproteins and growth factor receptors. Interestingly, some differentiated cell types, such as erythrocytes and platelets contain high amounts of phosphotyrosine. We analyzed tyrosine kinases expressed in the K-562 chronic myelogenous leukemia cell line, which has a bipotential erythroid and megakaryoblastoid differentiation capacity.

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“…K562 cells can undergo erythroid differentiation by treatment with hemin 10 and megakaryocytic differentiation with PMA, 11 and therefore this cell line has been used as representative models for studying erythroid and megakaryocytic differentiation. Based on the increased expression of HZF1 mRNA following induction of K562 cells with hemin or PMA, we hypothesized HZF1 may take part in erythroid differentiation and megakaryocytic differentiation of K562 cells.…”

Section: New Zinc Finger Protein Hzf1 and Its Function H Peng Et Almentioning

confidence: 99%

“…K562, an erythroleukemia cell line, can be differentiated into erythroid cells by treatment with hemin 10 and into megakaryocytic cells by treatment with phorbol myristate acetate (PMA), 11 and thus K562 cells have been used as representative models for the studies of erythroid or megakaryocytic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a novel zinc finger protein (HZF1) gene and its function in erythroid and megakaryocytic differentiation of K562 cells

Han

Zhang

2006

Leukemia

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A novel zinc finger protein (HZF1) gene was identified and characterized by screening a human bone marrow cDNA library, using a new expression sequence tag probe that contains sequences encoding zinc finger motifs. There are at least three transcripts that may result from different splicing of the pre-mRNA, but the differences among them are only involved in 5 0 non-translation region of HZF1 mRNA. HZF1 gene contains four exons and three introns. The putative protein consists of 670 amino-acid residues including 15 typical C2H2 and 2 C2RH zinc finger motifs. This structure characterization of HZF1 and the nuclear location of the protein suggest that HZF1 may function as a transcription factor. HZF1 mRNA expression was detected in ubiquitous tissues and various hematopoietic cell lines. Increased HZF1 mRNA expression was observed following erythroid differentiation of K562 cells induced by hemin or megakaryocytic differentiation of K562 cells induced by phorbol myristate acetate (PMA). Both of the antisense method and RNA interference assay revealed that repression of the intrinsic expression of HZF1 blocked the hemin-induced erythroid differentiation and reduced the PMA-induced megakaryocytic differentiation of K562 cells, which suggested that HZF1 play important roles in erythroid and megakaryocytic differentiation.

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K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin

Cited by 502 publications

References 13 publications

Hemoglobin Expression in Clones of K562 Cell Line

Hemoglobin Expression in Clones of K562 Cell Line

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Identification and characterization of a novel zinc finger protein (HZF1) gene and its function in erythroid and megakaryocytic differentiation of K562 cells

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