K88 variants K88ab, K88ac and K88ad in oral vaccination of different porcine adhesive phenotypes. Immunological aspects

Abstract

Bijlsma, I.G.W., van Houten, M., Frik, J.F., and Ruitenberg, E.J., 1987. K88 variants K88ab, K88ac and K88ad in oral vaccination of different porcine adhesive phenotypes. Immunological aspects. Vet.lmmunol.lmmunopathol.,16: 235-25D.Sows of different adhesive phenotypes were vaccinated orally during the last 4 weeks of gestation with K88-positive Escherichia coli. Sows susceptible to adhesion by the K88 variant of the vaccination strain produced a significant IgA-class specific anti-K88 response in colostrum and milk and post-farrowing serum. Indications for an IgM and IgG-class specific anti-K88 response were also found in this group but only in milk. In sows resistant to adhesion by the K88 variant of the vaccination strain only an IgA-class specific anti-KS8 antibody response was found in mammary secretions and in post-farrowing sera, but titres did not reach the high values of the former group. The response in the second group was attributed to the frequent administration of large quantities of K88-positive E.coli which to some extent can be compared with a colonization effect. Specificity for the serological components of the K88 variants was detectable in colostral IgA of sows susceptible to the vaccination strain only.

Pig vaccination strategies based on enterotoxigenic Escherichia coli toxins

“…Very early, in vitro and in vivo studies confirmed that fimbriae are highly immunogenic and that they could induce protecting antibodies inhibiting adhesion to enterocytes and colonization of the intestine [3]. The binding of fimbriae to their respective intestinal receptors is critical for the activation of mucosal immunity after oral immunization [22,23] Colostral antibodies induced following maternal immunization protect neonatal piglets. Based on this information, development of anti-fimbrial vaccines based on various fimbrial proteins was shown to be effective in protecting animals from neonatal diarrhea.…”

“…Very early, in vitro and in vivo studies confirmed that fimbriae are highly immunogenic and that they could induce protecting antibodies inhibiting adhesion to enterocytes and colonization of the intestine [ 3 ]. The binding of fimbriae to their respective intestinal receptors is critical for the activation of mucosal immunity after oral immunization [ 22 , 23 ]…”

Animal EnterotoxigenicEscherichia coli

“…A potential advantage of fimbriae of enteric pathogens is that they possess enteroadhesive properties, which they share with other mucosal immunogens such as the enterotoxins. The binding of fimbriae to their complementary intestinal receptors in the appropriate host species is important for the activation of mucosal immunity after oral immunization, as shown with the F4 fimbriae (531, 532). Other carrier bacteria have also shown some level of usefulness inducing anti-F5 antibodies including Lactobaccilus acidophilus (533).…”