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Abstract

Plan: The present study is aimed to carry out a phytochemical investigation on Cyperus rotundus Linn tubers and to conduct antimicrobial assays of the isolated phytoconstituents. Methodology: The ethanolic extract of Cyperus rotundus tubers was prepared and used for the isolation of phytoconstituents. The structures of all the isolated compounds were elucidated on the basis of spectral data analysis such as IR, 1 H-NMR, 13 C-NMR, Mass spectroscopy and chemical reactions. The isolated phytoconstituents were tested for antimicrobial activity against bacterial and fungal strains. Outcome: Phytochemical investigation of Cyperus rotundus tubers resulted in isolation of four phytoconstituents characterized as n-tritriacontan-16-one (1), Lup-12, 20 (29)-dien-3β-ol-3-α-L-arabinofuranosyl-2'-octadec-9"-eonate (2), npentadecanyl-9-octadecenoate (3) and n-tetradecanyl-n-octadec-9, 12-dienoate (4). Only compound 2 showed antimicrobial activity. Lup-12, 20 (29)-dien-3β-ol-3-α-L-arabinofuranosyl-2'-octadec-9"-eonate is a new phytoconstituent isolated for the first time from the plant source. Compound 1, 3 and 4 are reported first time from the Cyperus genus. Phytochemical investigation of tubers of Cyperus rotundus led to isolation of a new triterpenic constituent which may be used as chromatographic markers for quality control of the drugs.