Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) Ekstrak Etanol Daun Ketepeng Cina (Cassia alata L.) Terhadap Pertumbuhan Candida albicans

Gharnita, Yassie Shailla; Lelyana, Shelly; Sugiaman, Vinna Kurniawati

doi:10.28932/sod.v4i1.1766

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Flavonoids have been reported to inhibit bacterial growth through at least 3 different mechanisms. Flavonoids are capable of interrupting the production of nucleic acids, blocking the ability of bacteria to produce energy through the inhibition of metabolism, and binding to the protein of the cell membrane, leading to cell lysis (Rahman et al, 2017;Shailla Gharnita et al, 2019). Phenolic compounds inhibit bacterial growth by denaturing bacterial proteins.…”

Section: Extraction Of Kipahit Leavesmentioning

confidence: 99%

DETERMINATION OF THE MINIMUM INHIBITORY CONCENTRATION OF THE ETHANOLIC EXTRACT OF KIPAHIT LEAVES ON THE GROWTH OF Propionibacterium acnes

Wibowo,

Aida,

Nor

et al. 2023

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Acne is a skin disorder characterized by inflammation in the form of comedones, papules, pustules, and nodules. The disease is caused by the gram-positive bacterium, Propionibacterium acnes. Treatment of the disease using antibiotic agents such as erythromycin and clindamycin has been reported to cause some side effects. Therefore, alternative antibacterial agents derived from natural products are required to reduce the occurrence of side effects. Kipahit (Tithonia diversifolia A. Gray) is one of the plants with pharmacological activities, such as antibacterial and antiprotozoal activities. The aim of this study was to determine the minimum inhibitory concentration (MIC) of a 96% ethanolic extract of kipahit leaves on the growth of P. acnes. Kipahit leaves were extracted through the ultrasound-assisted extraction (UAE) method. Minimum inhibitory concentration (MIC) was determined using the liquid dilution method. A serial dilution was performed to prepare a series of extract concentrations: 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, and 0.39%. Whereas, clindamycin and DMSO 1% were used as positive and negative controls, respectively. Phytochemical screening showed that the 96% ethanolic extract of kipahit leaves contains flavonoids, alkaloids, saponins, steroids/triterpenoids, tannins, and phenolic compounds. In addition, the extract showed inhibitory activity against P. acnes at concentrations ranging from 25% to 1,56%. In contrast, the extract showed no inhibitory activity at concentrations of 0.78% and 0.39%. Further experiments confirmed that kipahit leaf extract acted as a bacteriostatic agent (inhibiting bacterial growth) at a minimum concentration of 1.56%. Keywords: Acne, Propionibacterium acnes, Kipahit, MIC

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Section: Extraction Of Kipahit Leavesmentioning

confidence: 99%

DETERMINATION OF THE MINIMUM INHIBITORY CONCENTRATION OF THE ETHANOLIC EXTRACT OF KIPAHIT LEAVES ON THE GROWTH OF Propionibacterium acnes

Wibowo,

Aida,

Nor

et al. 2023

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“…The test was carried out by growing the culture from each MIC test tube in a pour plate on sterile SDA media by taking 1 mL of the sample, growing it on SDA media and incubating at temperature of 37°C for 24 hours, then the growth of the fungus was observed by counting the number of colonies using a colony counter. MIC was indicated by the absence of fungal colonies growing on the media [33].…”

Section: Testing Minimum Inhibitory Concentration (Mic) and Minimum F...mentioning

confidence: 99%

Antifungal Activity of Moringa (Moringa Oleifera Lamk.) Seed Oil from East Nusa Tenggara, Indonesia, against Malassezia Furfur Causes Seborrheic Dermatitis

2023

Journal of Hunan University Natural Sciences

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Hair is skin adnexa in the form of a dermis layer that functions to protect the scalp from environmental influences such as ultraviolet rays, temperature, and humidity. Oily hair can trigger the growth of Malassezia furfur (M. furfur), which causes seborrheic dermatitis. Moringa seed oil contains phytosterol compounds and fatty acids, which can inhibit the growth of fungi. This study aimed to determine the antifungal activity of moringa seed oil from East Nusa Tenggara, Indonesia, against M. furfur. The study was conducted in vitro using the well diffusion method with inhibition zone parameters, minimum inhibitory concentration test by liquid dilution, and minimum fungicidal concentration test by counting the number of colonies. Moringa seed oil at concentrations of 7.5, 10, and 12.5% had antifungal activity against M. furfur with inhibition zones of 14.5 ± 1.32 mm (strong), 18.3 ± 3.51 mm (strong), and 26.3 ± 2.36 mm (very strong) with a minimum inhibitory concentration of 2.50% and a minimum fungicidal concentration of 5.00%. Moringa seed oil can be developed as an antifungal, where the greater the concentration, the greater the inhibitory and killing power against M. furfur. The novelty of this study is the antifungal activity test of moringa seed oil from East Nusa Tenggara that was obtained by a cold pressed method targeting the fungus M. furfur ATCC 14521 using the well diffusion method.

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Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) Ekstrak Etanol Daun Ketepeng Cina (Cassia alata L.) Terhadap Pertumbuhan Candida albicans

Cited by 2 publications

References 7 publications

DETERMINATION OF THE MINIMUM INHIBITORY CONCENTRATION OF THE ETHANOLIC EXTRACT OF KIPAHIT LEAVES ON THE GROWTH OF Propionibacterium acnes

DETERMINATION OF THE MINIMUM INHIBITORY CONCENTRATION OF THE ETHANOLIC EXTRACT OF KIPAHIT LEAVES ON THE GROWTH OF Propionibacterium acnes

Antifungal Activity of Moringa (Moringa Oleifera Lamk.) Seed Oil from East Nusa Tenggara, Indonesia, against Malassezia Furfur Causes Seborrheic Dermatitis

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