Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats

Yu, Haichao; Zeng, Rong; Lin, Yuanyuan; Li, Xin; Tasneem, Shumaila; Yang, Zhen; Qiu, Yixing; Li, Bin; Wang, Yuhong; Cai, Xiong; Wang, Wei

doi:10.1016/j.phymed.2019.152876

Cited by 37 publications

(16 citation statements)

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“…Moreover, infiltration of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6 and IL-17, inflammatory mediators augment like COX-2 and 5-LOX, reduction of antiinflammatory factors such as IL-4 and IL-10, which have been positively related to RA, causes synovial inflammation and cartilage damage (Jing et al, 2019;Li et al, 2019c;Lin et al, 2013;Pan et al, 2017). In RA, TNF-α, IL-1β, IL-6 and IL-17 play a decisive and synergistic role in synovial inflammation and cartilage damage (Jing et al, 2019;Yu et al, 2019;Saleem et al, 2020). In addition, the overproduction of TNF-α elevates the levels of IL-1β and IL-6, and generates matrix degrading enzymes (Jing et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…IL-17 also plays a pivotal role in RA, which promotes the overproduction of proinflammatory cytokines and MMPs, as well as the activation of the osteoclasts and angiogenesis (Jing et al, 2019). Based on the above, therapeutic substances that particularly impede the production of TNF-α, IL-1β, IL-6 and IL-17 distinguish a crucial target for RA treatment (Jing et al, 2019;Rui et al, 2019;Yu et al, 2019). IL-4 and IL-10 by contrast, are two pivotal anti-inflammatory cytokines, which also play an important role in regulating the levels of endogenous pro-inflammatory cytokines during RA (Jing et al, 2019;Saleem et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, 5-LOX is the decisive enzyme involved in the synthesis of leukotriene, which is directly responsible for RA diseases (Lin et al, 2013, Lin et al, 2014. In addition, MMPs belong to the family of proteolytic enzymes, which play a crucial role during RA and are primarily responsible for bone and cartilage erosion (Jing et al, 2019;Yu et al, 2019). Our results demonstrate that PBMC levels of COX-2, 5-LOX and MMP-2 are highly expressed in AIA model rats, while a significant decrease is observed in PH-treated rats.…”

Section: Discussionmentioning

confidence: 99%

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Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

et al. 2020

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Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease without effective and beneficial drugs. Many traditional folk medicines have been proven to be effective in treating RA. Among these, the root of Pterospermum heterophyllum Hance has been widely used as a traditional remedy against RA in China, but there is no scientific basis yet. The aim of this study was to investigate for the first time the chemical compositions and therapeutic effect of P. heterophyllum on adjuvant-induced arthritis (AIA) model in rats. 73 compounds were identified from P. heterophyllum based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-qTOF-MS/MS), and flavonoids may be partly responsible for the major anti-arthritic effect. In parallel, the P. heterophyllum extract at 160, 320, and 640 mg/kg/day were orally administered to rats for 22 days after post-administration adjuvant. The results showed that P. heterophyllum remarkably ameliorated histological lesions of the knee joint, increased body weight growth, decreased arthritis score, reduced thymus and spleen indices in model rats. Moreover, P. heterophyllum treatment persuasively downregulated the levels of rheumatoid factor (RF), C-reactive protein (CRP), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-17, cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) and matrix metalloproteinase-2 (MMP-2), and observably upregulated IL-4 and IL-10 levels in model rats. These findings suggest that P. heterophyllum has a prominent anti-RA effect on AIA rats by modulating the inflammatory responses, and supports the traditional folk use of this plant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

et al. 2020

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“…The increasing number of RA patients and their families are suffering a substantial burden related to socioeconomic costs, progressive disability, and even early death [ 2 ]. A series of continuously developing therapeutic solutions are used in clinical trials against RA, including non-steroidal anti-inflammatory drugs, disease-modifying anti-rheumatic drugs (DMARDs) of synthetic origin, and of biological origin [ 1 , 3 ]. However, the conventional drugs present low effectiveness and side effects that require the researchers to seek new therapeutic substitutes.…”

Section: Introductionmentioning

confidence: 99%

A Dual Target-Directed Single Domain-Based Fusion Protein Against Interleukin-6 Receptor Decelerate Experimental Arthritis Progression Via Modulating JNK Expression

et al. 2021

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The currently used anti-cytokine therapeutic antibodies cannot selectively neutralize pathogenic cytokine signalling that cause collateral damage to protective signalling cascades. The single domain chain firstly discovered in Camelidae displays fully functional ability in antigen-binding against variable targets, which has been seemed as attractive candidates for the next-generation biologic drug study. In this study, we established a simple prokaryotic expression system for a dual target-directed single domain-based fusion protein against the interleukin-6 receptor and human serum, albumin, the recombinant anti-IL-6R fusion protein (VHH-0031). VHH-0031 exhibited potent anti-inflammatory effects produced by LPS on cell RAW264.7, where the major cytokines and NO production were downregulated after 24 h incubation with VHH-0031 in a dose-dependent manner. In vivo , VHH-0031 presented significant effects on the degree reduction of joint swelling in the adjuvant-induced arthritis (AIA) rat, having a healthier appearance compared with the dexamethasone. The expression level of JNK protein in the VHH-0031 group was significantly decreased, demonstrating that VHH-0031 provides a low-cost and desirable effect in the treatment of more widely patients.

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“…Kadsura heteroclita is a vine plant primarily distributed in the southwest part of China, has been used in Traditional Chinese Medicine (TCM) as “Hai Feng Teng” or “Ji Xue Teng” for the treatment of menstrual irregularities and blood deficiencies [13], by activating the blood circulation, relieving pain, and eliminating dampness. In the “Hu Ping” and “Xue Feng” mountain areas of northwest Hunan province, the stems of K. heteroclita are used to treat traumatic injuries and rheumatoid arthritis by one of the Chinese ethnic minorities known as the Tujia people [14,15]. Traditionally they named the stems of K. heteroclita as “Xue Tong” which means “circulating the blood system”, which is used to treat blood related diseases [16].…”

Section: Introductionmentioning

confidence: 99%

New Cadinane Sesquiterpenes from the Stems of Kadsura heteroclita

et al. 2019

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As part of our continual efforts to exploit ‘Tujia Ethnomedicine’ for their pharmacophoric functionalities, we herein investigated Kadsura heteroclita collected from a deep Wulin mountain area in northern Hunan province. The current study resulted in the isolation of three new sesquiterpenes: 6α,9α,15-trihydroxycadinan-4-en-3-one (1), (+)-3,11,12-trihydroxycalamenene (2), (–)-3,10,11,12-tetrahydroxy-calamenene (3), along with four known sesquiterpenes (4–7), and a cytochalasin H (8). Their chemical structures were elucidated by 1D-, and 2D-NMR spectroscopy, and HRESI-MS, CD spectrometry. The antioxidant, and cytotoxic activities of the compounds were evaluated. Compound 8 exhibited a strong antioxidant effect with an IC50 value of 3.67 µM on isolated human polymorphonuclear cells or neutrophils.

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Kadsura heteroclita stem suppresses the onset and progression of adjuvant-induced arthritis in rats

Cited by 37 publications

References 38 publications

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

A Dual Target-Directed Single Domain-Based Fusion Protein Against Interleukin-6 Receptor Decelerate Experimental Arthritis Progression Via Modulating JNK Expression

New Cadinane Sesquiterpenes from the Stems of Kadsura heteroclita

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