Kaempferol Derivatives from Hippophae rhamnoides Linn. Ameliorate H₂O₂‐Induced Oxidative Stress in SH‐SY5Y Cells by Upregulating Nrf2

Abstract

As a medicinal and edible food, Hippophae rhamnoides Linn. subsp. sinensis Rousi is plenty of bioactive secondary metabolites including flavonoids and their derivatives, which have a certain protective effect on oxidative damage. The present study isolated three new kaempferol derivatives (compound 1‐3), named Hippophandine A‐C, from H. rhamnoides seed residue. The structures of Hippophandine A‐C were explicated by HR‐ESI‐MS, NMR, and chemical analyses. Hippophandine A‐C (1, 5, and 10 µM) were used to attenuate H2O2‐induced cell death in SH‐SY5Y and explore the mechanism in oxidative damage. The results elucidated that Hippophandine A‐C reduced MDA, and increased the relative SOD, GSH, CAT contents, Nrf2 and HO‐1 expression in H2O2‐induced SH‐SY5Y.