Kainate binding sites in the hippocampal mossy fibers: Localization and plasticity

Represa, Alfonso; Tremblay, E.; Ben‐Ari, Yehezkel

doi:10.1016/0306-4522(87)90237-5

Cited by 269 publications

(153 citation statements)

References 28 publications

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“…The high levels of kainate receptors in the CA3 subregion of the rat hippocampus (Hollmann and Heinemann, 1994) is in accordance with observations that the CA3 pyramidal cells are particularly sensitive to kainate receptor agonists-induced excitotoxicity (Nadler et al, 1978;Coyle, 1983;Represa et al, 1987). The best evidence for presynaptic kainate receptors was obtained through lesion studies.…”

Section: Excitotoxicitysupporting

confidence: 83%

“…The presence of functional presynaptic kainate receptors in the CA3 subregion of the rat hippocampus is supported by several biochemical and physiological observations (Represa et al, 1987;Ben-Ari and Gho, 1988) and by our demonstration, for the first time, that kainate receptor stimulation increases the [Ca2+]i in hippocampal synaptosomes . However, there are no clear immunocytochemical demonstrations of these presynaptic receptors, which may be due, in part, to conformational changes, to association of the receptors with other molecules, or to the presence of undetectable low levels of kainate receptor subunits (Petralia, 1997).…”

Section: Immunocytochemandtrysupporting

confidence: 72%

“…Both in mice and in rat hippocampal CA3 subregion, there exist a large number of kainate binding sites in the stratum lucidum, corresponding to the mossy fiber nerve terminals region (Foster et al, 1981;Unnerstall and Wamsley, 1983;Represa et al, 1987;Miller et al, 1990;Garcia-Ladona and Gombos, 1993;Frotscher et al, 1994). The presence of high-affinity kainate binding sites in the stratum lucidum of the rat CA3 subregion is an indication of a synaptic localization of high-affinity kainate receptors.…”

Section: [~H]kainate Bindin9 Sitesmentioning

confidence: 99%

“…The best evidence for presynaptic kainate receptors was obtained through lesion studies. It was observed that [3H]kainate binding sites in the stratum lucidum of the rat hippocampus are more sensitive to colchicine lesion of granule cells than to kainate induced CA3 pyramidal cells death (Represa et al, 1987). Also, neonatal ~-ray irradiation reduce the number of granule cells and prevent the epileptic action of kainate (Gaiarsa et al, 1994).…”

Section: Excitotoxicitymentioning

confidence: 99%

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Kainate receptors in hippocampal CA3 subregion: evidence for a role in regulating neurotransmitter release

Malva

Carvalho

1998

Neurochemistry International

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The hippocampal CA3 subregion of the rat is characteristically enriched in kainate receptors. At the synaptic level, the subcellular localization of these receptors is still a matter of debate. The CA3 pyramidal cells are particularly sensitive to excitotoxicity induced by kainate, which is in agreement with the high levels of kainate receptors in the stratum lucidum of the hippocampal CA3 subregion. Immunocytochemical studies, using antibodies against kainate receptor subunits, clearly demonstrated the presence of postsynaptic kainate receptors. However, it was not possible at the time to identify the activity of postsynaptic kainate receptors as mediators of the synaptic transmission. There are also reports showing the labeling of unmyelinated axons and nerve terminals with antibodies against kainate receptor subunits. The evidence for the presence of presynaptic kainate receptors in the hippocampus is further substantiated by the demonstration that stimulation of kainate receptors in synaptosomes isolated from the rat hippocampal CA3 subregion increases the intracellular free Ca 2÷ concentration ([Ca2+]~) coupled to the release of glutamate. These results support the model proposed by Coyle (1983), in which the excitotoxicity induced by kainate involves the activation of presynaptic kainate receptors, causing the release of glutamate. According to this model, the neurotoxic effect of kainate in the rat hippocampal CA3 subregion involves a direct effect on presynaptic kainate receptors and an indirect effect on postsynaptic glutamate receptors due to the enhanced release of glutamate.

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Section: Excitotoxicitysupporting

confidence: 83%

Section: Immunocytochemandtrysupporting

confidence: 72%

Section: [~H]kainate Bindin9 Sitesmentioning

confidence: 99%

Section: Excitotoxicitymentioning

confidence: 99%

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Kainate receptors in hippocampal CA3 subregion: evidence for a role in regulating neurotransmitter release

Malva

Carvalho

1998

Neurochemistry International

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“…TLE is associated with abnormal sprouting of recurrent mossy fibres, which synapse onto dentate gyrus cells [130][131][132] and this abnormal sprouting is also reduced in GluK2 −/− mice [133]. Consistent with the requirement for recruitment of KARs to postsynaptic sites for seizure activity, despite induction of MF sprouting in an animal model of TLE, C1ql2/3 double KO mice are resistant to seizures [89] and genetic silencing of KARs at CA3 synapses attenuates kainate-induced seizures [134].…”

Section: Epilepsymentioning

confidence: 87%

Exciting Times: New Advances Towards Understanding the Regulation and Roles of Kainate Receptors

et al. 2017

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Kainate receptors (KARs) are glutamate-gated ion channels that play fundamental roles in regulating neuronal excitability and network function in the brain. After being cloned in the 1990s, important progress has been made in understanding the mechanisms controlling the molecular and cellular properties of KARs, and the nature and extent of their regulation of wider neuronal activity. However, there have been significant recent advances towards understanding KAR trafficking through the secretory pathway, their precise synaptic positioning, and their roles in synaptic plasticity and disease. Here we provide an overview highlighting these new findings about the mechanisms controlling KARs and how KARs, in turn, regulate other proteins and pathways to influence synaptic function.

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Rapid induction by kainic acid of both axonal growth and F1/GAP-43 protein in the adult rat hippocampal granule cells

Cantallops

Routtenberg

1996

J. Comp. Neurol.

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Hippocampal granule cells do not normally express the axonal growth- and plasticity-associated protein F1/GAP-43 in the adult rat. Using three different methods that lead to hypersynchronous activity in limbic circuits, expression of F1/GAP-43 mRNA can be induced in granule cells which is followed by sprouting in mossy fibers, the axons of granule cells. F1/GAP-43 mRNA expression in granule cells was induced in the temporal, but not septal, hippocampus beginning at 12 hours after kainic acid (KA) subcutaneous injection (10 mg/kg). Beginning 2 days after KA treatment, mossy fiber sprouts restricted to the temporal hippocampus were observed in the supragranular layer. In the same animal we also observed that levels of protein F1/GAP-43 immunoreactivity in this layer apparently increased at this same 2 day time point and same ventral hippocampal location. F1/GAP-43 protein levels and mossy fiber sprouting showed an increase up to 10 days after KA treatment. Sprouting was at a maximum at 40 days, the longest time point studied. These events parallel axonal regeneration with one critical difference: granule cell axons are not damaged by kainate. The rapid onset of axonal growth in the adult is striking and occurs earlier than reported previously (2 days vs. 12 days). Such growth closely associated with elevated levels of protein F1/GAP-43 may occur as a result of a) reactive synaptogenesis caused by the availability of post-synaptic surface on granule cell dendrites at the supragranular layer, b) Hebbian co-activation of the post-synaptic granule cells and their presynaptic afferents, and c) loss of target-derived inhibitory growth factor.

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Kainate binding sites in the hippocampal mossy fibers: Localization and plasticity

Cited by 269 publications

References 28 publications

Kainate receptors in hippocampal CA3 subregion: evidence for a role in regulating neurotransmitter release

Kainate receptors in hippocampal CA3 subregion: evidence for a role in regulating neurotransmitter release

Exciting Times: New Advances Towards Understanding the Regulation and Roles of Kainate Receptors

Rapid induction by kainic acid of both axonal growth and F1/GAP-43 protein in the adult rat hippocampal granule cells

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