Kaistella gelatinilytica sp. nov., a flavobacterium isolated from Antarctic soil

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, non-motile, yellow-pigmented bacteria, designated strain G5-32T, belonging to the genus Kaistella was isolated from soil collected in the Antarctic. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0–5% (w/v) NaCl (optimum, 1%), at pH 6.0–9.0 (optimum, pH 8.0) and at 4–28 °C (optimum, 20 °C). The predominant menaquinone was MK-6 (99.4%). The major fatty acids were anteiso-C15:0 (28.2%), iso-C15:0 (16.4%), summed feature 9 (comprising iso-C17:1 ω9c and/or 10-methyl C16:0; 10.6%) and iso-C16:0 (5.9%). A phylogenetic tree based on 16S rRNA gene sequences showed that strain G5-32T formed a lineage within the genus Kaistella with the closest phylogenetic neighbours Kaistella yonginensis HMD1043T, Kaistella chaponensis DSM 23145T, Kaistella jeonii DSM 17048T and Kaistella carnis NCTC 13525T (97.9, 97.8, 97.8 and 98.0 % 16S rRNA gene sequence similarity, respectively). The ANI values between strain G5-32T and K. jeonii DSM 17048T, K. chaponensis DSM 23145T, K. carnis NCTC 13525T and K. yonginensis HMD1043T were 90.9, 82.6, 77.1 and 76.3%. Concurrently, digital DNA–DNA hybridization values of strain G5-32T assessed against K. jeonii DSM 17048T, K. chaponensis DSM 23145T, K. carnis NCTC 13525T and K. yonginensis HMD1043T were 42.3, 25.9, 21.7 and 21.3%, respectively. Based on phenotypic, phylogenetic and genotypic data, a novel species, Kaistella gelatinilytica sp. nov., is proposed. The type strain is G5-32T (=CCTCC AA 2019083T=KCTC 72766T).

Kaistella rhinocerotis sp. nov., isolated from the faeces of rhinoceros and reclassification of Chryseobacterium faecale as Kaistella faecalis comb. nov.

“…The quinones were eluted using a mobile phase consisting of a methanol-isopropyl ether solution (3 : 1, v/v) at a flow rate of 0.7 ml min −1 [30]. Menaquinone-6 (MK-6) was identified as the major respiratory quinone of Ran72 T , which is typical of the genus Kaistella [1,[11][12][13]. Strain Ran72 T and the other species were cultured under identical conditions to analyse the fatty acid methyl ester profiles during exponential growth.…”

“…Therefore, further characterization of Kaistella strains isolated from rhinoceroses could provide valuable insights into the broader microbiota of herbivores. Moreover, members of the genus Kaistella have primarily been isolated from various environments, including freshwater, Antarctic soil, and oil-contaminated experimental soil [1, 11–13]. Kaistella species derived from aquatic habitats (e.g., Kaistella jeonii ) are known to possess the potential to degrade polyethylene terephthalate by colonizing its surfaces and harbouring esterases (e.g., serine hydrolases) [14].…”

Kaistella polysaccharea sp. nov., isolated from Antarctic intertidal sediment produces a novel extracellular polymeric substance

“…Symbols: +, positive; -, negative. *G+C content of the closely related type strains were taken from [53], [54], [55] and [56].…”

Kaistella yananensis sp. nov., a Novel Indoleacetic Acid-Producing Bacterium Isolated from the Root Nodules of Sophora davidii (Franch.) Skeels

“…The members of Flavobacteriia isolated from soil have potential agricultural utility, such as plant protection against pathogens and secretion plant growth-promoting substances (Marques et al 2010; Sang and Kim 2012). Members of Kaistella have been isolated from human clinical sources (Yassin et al 2010), water (Kim et al 2004), soil (Ren et al 2019) and sediment (Pires et al 2010). At present, the genus Kaistella consists of 16 species with validly published names: Kaistella antarctica ( In a screen for bacterial strains with plant growth-promoting traits that may facilitate phytoremediation, strain BT6-1-3 T , which can produce indole-3-acetic acid, was isolated from root nodules of Sophora davidii (Franch.)…”