Kallar grass <i>(Leptochloa fusca L. Kunth)</i> as a feedstock for ethanol fermentation with the aid of response surface methodology

Gul, Ambreen; Irfan, Muhammad; Nadeem, Muhammad; Syed, Quratulain; Haq, Ikram Ul

doi:10.1002/ep.12701

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…process's main objective is to decrease the degree of polymerization of cellulose by hydrolyzing the large polysaccharides to simple sugar yeast to produce bioethanol [28]. The graph in Figure 7 is in line with the previous study by Gul et al, where the highest concentration of bioethanol from Kallar grass by Kluyveromyces marxianus was obtained at 32 h fermentation time, and the concentration tends to decrease [29]. This result indicates that bamboo can produce bioethanol optimally after the combination of MW pretreatment and hydrolysis by cellulase enzyme were done.…”

Section: Effect Of Mw Pretreatment On Ssfsupporting

confidence: 88%

Effect of Microwave Pretreatment on Some Properties of Bamboo (Gigantochloa apus) for Bioethanol Production

Harahap¹,

Husnil²,

Ramadhan³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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Pretreatment of lignocellulosic biomass plays an essential role in bioethanol production as an alternative biofuel. This process reduces biomass recalcitrance in order to improve cellulose digestibility for saccharification and further fermentation reactions. In this study, microwave (MW) pretreatment was done on bamboo (Gigantochloa apus) to investigate the resulting physicochemical properties and bioethanol produced. Bamboo was irradiated in the microwave at different power (100-600 Watt) and irradiation time (5-20 minutes) followed by Water Soluble content (WSC) and lignin analysis. Simultaneous saccharification and fermentation (SSF) using cellulase enzyme was also done in five different treatment combinations (C1-C5) to investigate the effect of produced reducing sugar and bioethanol. The result shows that increasing MW power and irradiation time could decrease WSC gradually. The lowest WSC of 0.3% was obtained at 600-Watt MW power and 20 minutes irradiation time. Lignin content decreased from 18.9% to 16.0% concerning increasing irradiation time from 5 to 20 minutes under 300-Watt MW power. SEM images show that partial disruption and micro-scale pores existed in pretreated samples. The highest amount of ethanol was obtained at 24 hours fermentation for pretreated bamboo at 300-Watt MW power for 15 minutes followed by cellulase enzyme addition. The overall results showed that microwave pretreatment is a prospective method for future bioethanol production from bamboo due to effective WSC reduction and lignin degradation in a relatively short period of time.

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Section: Effect Of Mw Pretreatment On Ssfsupporting

confidence: 88%

Effect of Microwave Pretreatment on Some Properties of Bamboo (Gigantochloa apus) for Bioethanol Production

Harahap¹,

Husnil²,

Ramadhan³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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“…In this context, bioethanol from lignocellulosic biomass (LCB) is considered to be one of the important promising alternative liquid fuels [2]. The use of lignocellulosic biomass as feedstock for bioethanol production [3] has certain advantages over the fossil fuels. LCB has a high potential for ethanol production, they are abundant renewable resources, has a variety of source, do not compete with the first generation of biofuel, and could integrate as Integrated Biomass Utilization System (IBUS) [4].…”

Section: Introductionmentioning

confidence: 99%

Cellulosic bioethanol production from Eragrostis airoides Nees grass collected from Northeast India

Singh

2019

SN Appl. Sci.

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The aim of this study was to check the ethanol yield of locally available lignocellulosic biomass (Eragrostis airoides Nees) by enzymatic saccharification and microbial fermentation. Two types of physico-chemical pretreatments were studied (dilute acid and alkaline). Physico-chemical pretreatment with dilute acid showed to be superior over the alkaline treatment with respect to the rate of enzyme hydrolysis and ethanol productivity. In the pretreatment conditions, the 5% acid + autoclave yielded the highest concentrations of total reducing sugar (25.46 g/L) and enzymatic hydrolysis (9.13 g/L). Fermentation of the E. airoides hydrolyzates by Saccharomyces cerevisiae produced a fermentation yield of 17.56 g/L ethanol. Therefore, E. airoides could be utilized to produce bioethanol and it can be a potential candidate for future bioethanol production as suggested by the analysis.

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“…On the other hand, hydrolysis time showed the greatest impact on the sugar concentrations, and it was observed that longer hydrolysis time resulted in higher sugar concentrations for both enzymes (Figure 2b and 2c). Similarly, Gul et al (2018) showed that longer hydrolysis time caused higher saccharification efficiency from Kallar grass. Furthermore, Kshirsagar et al (2015) found the optimal conditions for reducing sugar yield from rice straw as 40 FPU/g enzyme and 17.50% biomass loading for 72 h when the researchers used RSM for the experiments.…”

Section: Response Surface Methodologymentioning

confidence: 95%

Response surface methodology-based optimization studies about bioethanol production by Candida boidinii from pumpkin residues

Demiray,

Karatay,

Dönmez

2024

Biotech Studies

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For sustainable bioethanol production, the investigation of novel fermentative microorganisms and feedstocks is crucial. In this context, the goals of the current study are suggesting pumpkin residues as new raw material for bioethanol production and investigating the fermentative capacity of the Candida boidinii, which is a newly isolated yeast from sugar factory wastes. Response surface methodology was used to determine the effect of enzyme (cellulase and hemicellulase) concentration and enzymatic hydrolysis time. The maximum bioethanol concentration was 29.19 g/L when fermentation parameters were optimized. However, it is revealed that enzymatic hydrolysis and hydrolysis duration (48-72 h) have significant effects on reducing sugar concentration. The highest reducing sugar was 108.86 g/L when the 20% initial pumpkin residue was hydrolyzed at 37.5 FPU/g substrate cellulase and 37.5 U/mL hemicellulase at the end of 72 h. Under these optimized conditions, the bioethanol production of C. boidinii increased by 22.91% and reached 35.88 g/L. This study shows pumpkin residues are promising feedstocks and C. boidinii is a suitable microorganism for efficient bioethanol production.

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Kallar grass (Leptochloa fusca L. Kunth) as a feedstock for ethanol fermentation with the aid of response surface methodology

Cited by 7 publications

References 27 publications

Effect of Microwave Pretreatment on Some Properties of Bamboo (Gigantochloa apus) for Bioethanol Production

Effect of Microwave Pretreatment on Some Properties of Bamboo (Gigantochloa apus) for Bioethanol Production

Cellulosic bioethanol production from Eragrostis airoides Nees grass collected from Northeast India

Response surface methodology-based optimization studies about bioethanol production by Candida boidinii from pumpkin residues

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