Kapok Fiber: A Natural Biomaterial for Highly Specific and Efficient Enrichment of Sialoglycopeptides

Abstract

Cancer development and chronic diseases are associated with the overexpression of sialoglycans terminated to the surface proteins and lipids of cancer cells compared with normal cells. The isolation and detection of sialoglycopeptides from complex peptides mixture still remain challenges due to their low abundance, low ionization, and losses of sialic acid residues and water molecule during analytical processes. In this study, kapok fiber, a natural fiber derived from the kapok tree (Bombax ceiba L.), has shown excellent capability to specifically and efficiently enrich sialoglycopeptides, without losses of sialic acid residues and water molecule from sialoglycans. The main components on the surface of kapok fiber are syringyl and guaiacyl lignins which play an important role in isolating sialoglycopeptides from complex peptide mixtures.

Mass spectrometry for protein sialoglycosylation

“…Sialylated glycopeptides are specifically captured by the magnetic nanoparticles via the weak anion exchange and hydrophilic interaction . Recently, two novel materials, kapok fiber and sugar‐integrated smart copolymer, are found to have high specificity toward sialylated glycopeptides through hydrogen bonding interactions . In particular, kapok fiber is a readily available natural biomaterial and can selectively enrich sialoglycopeptides without loss of sialic acid, showing good potential for analytical applications.…”

“…118 Recently, two novel materials, kapok fiber and sugar-integrated smart copolymer, are found to have high specificity toward sialylated glycopeptides through hydrogen bonding interactions. 119,120 In particular, kapok fiber is a readily available natural biomaterial and can selectively enrich sialoglycopeptides without loss of sialic acid, showing good potential for analytical applications. However, although these techniques have shown some advantages in isolating sialylated glycopeptides from complex biological mixtures, simple and high throughput protocols for routine enrichment are still currently lacking.…”

Bio‐based polydimethylsiloxane porous sponge materials with programmable hydrophobicity and porosity for efficient separation of hydrophobic liquids from water

“…In the FTIR spectra of the fibers, peaks at 3344 (OH), 1731 (C═O), and 1243 cm −1 (CO) were observed suggesting the presence of cellulose as major constituent. The peak with maximum intensity was observed at 1039 cm −1 (CC) along with a peak at 2919 cm −1 is assigned to the symmetric and asymmetric aliphatic CH 3 and CH 2 suggesting the presence of possible plant wax in fiber matrix 58 (Figure 1b). The fibers appear golden white in color under visual appearance and have a joint end to both the sides of the seed (Figure 1c).…”

Modification Strategies of Kapok Fiber Composites and Its Application in the Adsorption of Heavy Metal Ions and Dyes from Aqueous Solutions: A Systematic Review

“…Fourier transform infrared (FTIR) results of raw KF show different functional groups that are present in the fiber. The band at 3404 cm −1 is associated with hydroxyl stretching from plant wax, fatty acids and water [ 52 , 67 , 68 ]. Cellulosic components CH 2 and CH 3 are found at band 2917 cm −1 [ 67 , 69 ].…”