Karakterisasi dan Evaluasi Koleksi Sumberdaya Genetik Durian Berdasarkan Karakter Morfologi Buah

Hadiati, Sri; Nasution, Fitriana; Kuswandi, nFN

doi:10.21082/blpn.v22n1.2016.p1-10

Cited by 2 publications

(3 citation statements)

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“…Terhitung sebanyak 103 varietas durian telah dibudidayakan di Indonesia (Ruwaida et al, 2009). Nama-nama varietas di setiap daerah berbeda-beda, tetapi belum tentu memiliki perbedaan genetik yang besar (Hadiati & Nasution, 2016), sehingga pengetahuan dan data keanekaragaman durian ini menjadi terbatas. Hal ini menjadi kendala saat ini dalam pengelolaan dan pengembangan durian di Indonesia.…”

Section: Pendahuluanunclassified

“…Keanekaragaman genetik durian dan kerabatnya telah dievaluasi secara morfologi (Lestari et al, 2011;Baroroh et al, 2014;Hadiati & Nasution, 2016;Priyanti, 2016;Naufal, 2021) dan molekuler dengan berbagai marka, diantaranya Random Amplified Polymorphic DNA (RAPD) pada durian 'Sukun' (Ruwaida et al, 2009); Inter Simple Sequence Repeats (ISSR) pada D. tanjungpurensis (Riupassa et al, 2015); ISSR pada durian (Syahruddin, 2012;Vanijajiva, 2012) Secara morfologi, organ yang digunakan untuk analisis keanekaragaman durian adalah daun. Organ daun pada tumbuhan sering diamati karena keberadaannya yang tidak bergantung terhadap musim, daya regenerasinya yang cepat, dan ciri morfologinya mudah diamati dibandingkan organ lain (Syahruddin, 2012).…”

Section: Pendahuluanunclassified

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Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR

Fitri

Priyanti

Dasumiati

2023

Al-Kauniyah J. Biol.

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AbstrakData keanekaragaman durian (Durio zibethinus L.) yang terbatas merupakan salah satu kendala yang dihadapi dalam pengelolaan dan pengembangannya di Indonesia. Keanekaragaman genetik dapat ditinjau berdasarkan ciri morfologi yang didukung dengan ciri molekuler. Tujuan penelitian ini adalah untuk mengetahui kekerabatan antar aksesi berdasarkan dendrogram yang menggabungkan data morfologi daun dan polimorfisme Inter Simple Sequence Repeats (ISSR). Pengamatan ciri morfologi mengacu pada deskriptor durian dan ektraksi DNA dengan metode CTAB yang telah dimodifikasi. Setelah berhasil diekstraksi, DNA durian diamplifikasi dengan tujuh primer ISSR untuk analisis polimorfisme. Konstruksi dendrogram yang terbentuk merupakan hasil analisis menggunakan program NTSYS. Berdasarkan 15 ciri morfologi yang diamati terdapat 5 ciri yang sangat membedakan antara kelompok aksesi durian. Lai (D. kutejensis) memiliki daun lebih panjang hingga 24 cm dan lebar hingga 16 cm dibandingkan 28 aksesi durian dengan panjang hingga 16 cm dan lebar hingga 9 cm. Hasil amplifikasi diperoleh sebanyak 35 pita dan 31 pita bersifat polimorfik dengan persentase polimorfisme berkisar antara 66,7–100%. Persentase polimorfisme pada penelitian ini dapat mencapai 100% dengan primer ISSR 842, PKBT 4, dan PKBT 5. Konstruksi dendrogram ciri morfologi mempunyai koefisien kemiripan sebesar 0,56–0,91 dan membentuk dua kelompok yang tidak memisahkan aksesi-aksesi durian dengan Lai. Ciri polimorfisme ISSR dapat digunakan untuk merekonstruksi dendrogram menjadi dua kelompok dengan koefisien kemiripan sebesar 0,48–0,96. Dendrogram ciri polimorfisme ISSR secara tegas memisahkan aksesi-aksesi durian dengan Lai. Pohon kekerabatan 28 aksesi durian dan Lai di Kecamatan Serpong telah direkonstruksi untuk pertama kalinya.AbstractLimited data on the diversity of durian (Durio zibethinus L.) is one of the obstacles encountered in its management and development in Indonesia. Genetic diversity can be reviewed based on morphological characteristics supported by molecular characteristics. This study aimed to determine the relationship between accessions based on a dendrogram that combines leaf morphology and Inter Simple Sequence Repeats (ISSR) polymorphism data. Observation of morphological features refered to durian descriptors and DNA extraction using the modified CTAB method. After successful extraction, durian DNA was amplified with seven ISSR primers for polymorphism analysis. The dendrogram construction is formed the NTSYS program. Based on the 15 morphological characteristics observed, 5 characteristics greatly distinguished the durian accession group. Lai (D. kutejensis) had longer up to 24 cm and wider up to 16 cm leaves than 28 durian accessions with a length (16 cm) and width (9 cm). The amplification results were obtained 35 bands and 31 out of them were polymorphic with polymorphic PCR product ranged 66.7–100%. The percentage of polymorphism could reach 100% with ISSR 842, PKBT 4, and PKBT 5 primers. The dendrogram construction based on morphological characteristics had an interaction coefficient of 0.56–0.91 and formed two groups which did not separate durian accessions with Lai. The ISSR polymorphism feature can be used to reconstruct the dendrogram into two groups with a slope coefficient of 0.48–0.96. The dendrogram based on the ISSR polymorphism feature explicitly divides durian accessions with Lai. The cluster analysis of 28 durians and Lai accessions in Serpong District has been reconstructed for the first time.

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Section: Pendahuluanunclassified

Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR

Fitri

Priyanti

Dasumiati

2023

Al-Kauniyah J. Biol.

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“…In 2015, there were 98 kinds of durians with superior variety. There are more to find and release according to their origins name (Hadiati & Nasution, 2016).…”

Section: Agro-tourism Development Effect Towards Ecology Aspect In Dementioning

confidence: 99%

Agro-Tourism Development Strategy in Desa Wisata Kaligono (Dewi Kano) of Kaligesing District of Purworejo Regency

Pambudi

Sunarto

Setyono

2018

Agriekonomika Jurnal Sosial EKonomi dan Pertanian

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This research aims were: to gain information about villagers’ perception concerning to the plan of building an agro-tourism area in Dewi Kano, to find out the side effect of agro-tourism, to formulate the strategy in developing the agro-tourism. Researcher used observation, interview, questionnaire, and SWOT analysis (in formulating the agro-tourism development strategy) as methods. The results of the research show that the villagers of Dewi Kano are supporting the plan of developing their area as an agro-tourism; this is seen from their perceptions which tend to agree, because they understand that agro-tourism development can give benefits in ecology, economy, social, and management. The strategy which can be applied in developing agro-tourism of Dewi Kano is a progressive strategy; it means, the research location is in a great condition, so it will be well-developed by maximizing the opportunities.

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Karakterisasi dan Evaluasi Koleksi Sumberdaya Genetik Durian Berdasarkan Karakter Morfologi Buah

Cited by 2 publications

References 2 publications

Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR

Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR

Agro-Tourism Development Strategy in Desa Wisata Kaligono (Dewi Kano) of Kaligesing District of Purworejo Regency

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