Karakterisasi Keragaman Genetik 27 Genotipe Cabai Berdasarkan Marka SSR (Simple Sequence Repeat)

Terryana, Rerenstradika Tizar; Nugroho, Kristianto; Rijzaani, Habib; Lestari, Puji

doi:10.14203/beritabiologi.v17i2.3313

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…The individuals of pitcher plants showed an indication to form four groups. This shows that accessions located at one of the adjacent coordinates show a high level of genetic similarity (Terryana et al, 2018).…”

Section: Resultsmentioning

confidence: 81%

Similarity level of Nepenthes spp. based on the qualitative characters

Lukmanasari

Purwantoro

Murti

et al. 2020

ipas

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Nepenthes, also known as pitcher plant, are unique and interesting flora developed as ornamental plants. This species has an attraction not only on the flowers but also on the diverse shapes and colors of the pitchers. Each Nepenthes species and hybrid has a variety of characters. The genetic relationship and diversity in Nepenthes species and hybrids can be determined based on the morphological characterization. The purpose of this study was to determine the similarity level among the Nepenthes accessions based on 31 qualitative characters. In this study, 30 Nepenthes species and 11 hybrids were used. The range of the similarity coefficient of Nepenthes species was 0.28-0.65, and divided into two major groups namely group A and B. Group A was the Nepenthes population which had petioles consisted of 17 accessions, while group B had folius consisting of 24 accessions. It indicated that when the similarity value is higher, the similarity level between each plant becomes higher. Principle Coordinates Analysis (PCoA) provided an overview of the relationship among the genotypes in coordinates. Species with a high similarity coefficient were in a close position, whereas species with a low similarity coefficient were in a remote position. Based on the PCoA, the species were grouped based on the similar characters, which was supported by the dendrograms.

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Section: Resultsmentioning

confidence: 81%

Similarity level of Nepenthes spp. based on the qualitative characters

Lukmanasari

Purwantoro

Murti

et al. 2020

ipas

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“…Overall, the varied degrees of resistance to anthracnose observed in the pepper varieties in our study could be supported by further molecular analysis Analysis of molecular marker polymorphisms. We successfully uncovered 14 loci across the 15 pepper ned an average number of 7 alleles with a range of 3 alleles to 5 alleles per locus (Table 4), which is a lower number than that reported in a previous study that identified 179 alleles in 27 red pepper genotypes using 24 microsatellite markers [17]. The number September 2019  Vol.…”

Section: Anthracnose Symptoms Visualizedmentioning

confidence: 72%

“…successfully uncovered 14 loci across the 15 pepper varieties. We obtained an average number of 7 alleles with a range of 3 alleles to 5 alleles per locus (Table 4), which is a lower number than that reported in a previous study that identified 179 alleles in 27 red pepper gen types using 24 microsatellite markers [17]. The n the dip method to understand the physical resistance ].…”

Section: Anthracnose Symptoms Visualizedmentioning

confidence: 87%

“…However, in Indonesia, genetic diversity studies of pepper varieties using molecular markers-particularly those related to the anthracnose resistance character-are unfortunately rare. There are two previous studies that used microsatellite markers to analyze pepper diversity; one was based on the Begomovirus resistance character, and the other study using newly designed microsatellite markers was based on the results of total pepper genome sequencing [16,17]. We conducted this study to analyze the genetic diversity of Indonesian pepper varieties based on anthracnose resistance using molecular markers.…”

Section: Introductionmentioning

confidence: 99%

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The Use of Molecular Markers to Analyze the Genetic Diversity of Indonesian Pepper (Capsicum spp.) Varieties Based on Anthracnose Resistance

Nugroho¹,

Terryana²,

Manzila³

et al. 2019

Makara J. Sci

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Anthracnose is an important disease affecting the pepper plant and can lead to significant decreases in harvest yield. In this study, the genetic diversity of Indonesian pepper varieties was analyzed based on anthracnose resistance using molecular markers. DNA collected from 15 pepper varieties belonging to two species-Capsicum annuum L. and C. frutescens L.-were amplified using 14 molecular markers. The fungal isolate Colletotrichum capsici was inoculated into ripe harvested pepper fruits to observe their resistance to anthracnose as indicated by lesion size. Phylogenetic analysis revealed that the 15 pepper varieties could be classified into two major clusters with a genetic similarity coefficient of 0.63, and the pepper varieties exhibited varying degrees of resistance to anthracnose based on lesion size. Using the molecular markers, we were able to differentiate the species of pepper varieties, but not their resistance to anthracnose. All markers used in this study were confirmed to be highly informative (PIC > 0.5), suggesting their potential use in genetic studies on peppers. The marker GPMS29 was found to be significantly associated (P < 0.05) with anthracnose resistance. This information about the genetic diversity of peppers-along with the molecular markers used in our study-could prove to be useful in the further development of breeding programs of pepper plants in terms of anthracnose resistance in Indonesia.

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“…Pemanfaatan teknologi marka molekuler menghasilkan sidik jari DNA yang efektif dan efisien dalam mengidentifikasi varietas pada level DNA karena lebih akurat dan tidak dipengaruhi oleh faktor lingkungan (Risliawati et al 2015;Gao et al 2016). Marka molekuler juga telah banyak digunakan pada analisis diversitas genetik dengan presisi tinggi dan untuk memprediksi latar belakang genetik varietas (Ribeiro et al 2013;Lestari et al 2016;Terryana et al 2018). Di samping itu, marka molekuler dapat digunakan sebagai pelengkap pada uji kebaruan, keunikan, keseragaman, dan kestabilan (BUSS) melalui perbandingan profil sidik jari DNA.…”

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Genetic Diversity Analysis and Development of DNA Fingerprints of 20 Indonesian Local Chili Pepper Varieties Based on SSR Markers

Terryana¹,

Ningrum²,

Nugroho³

et al. 2020

J. AgroBiogen

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Chili pepper is one of the most valuable horticultural crops, widely cultivated in Indonesia. Analysis of its genetic diversity is needed to develop successful breeding programs of local varieties. Simple sequence repeat (SSR), a robust molecular marker used for genetic diversity analysis in plant species, offers potential, reliable DNA fingerprinting method to assess genetic variation and varietal identification of chili pepper. Fifteen SSR markers were used in this study to analyze the genetic diversity and develop profiling identification of DNA fingerprint of local chili pepper varieties. Twenty local and two improved varieties of three chili pepper species, consisting of 3, 1, and 18 varieties of Capsicum frutescens, C. chinense, and C. annuum, respectively, were assessed for their SSR polymorphism. A total of 87 alleles was obtained from the polymorphism analysis with high alleles variation (2–16 alleles) with average total allele of 5.8 and average polymorphism information content (PIC) of 0.59 (0.34–0.83). Clustering and Principle Coordinate Analyses (PCoA) classified the varieties into two groups with coefficient of similarity of 0.65 indicating their high genetic variability. Most local varieties belonged to the same cluster and separated from the two improved varieties. Based on PIC values and dendrogram with selected markers, five SSR markers, i.e. EPMS441, EPMS331, EPMS335, GPMS194, and CaSSRBio1.1, were identified as SSR marker set for DNA fingerprinting purposes. SSR marker set used in this study was successful in developing the varietal identity of local chili pepper varieties, as indicated by unique code of each variety.

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Karakterisasi Keragaman Genetik 27 Genotipe Cabai Berdasarkan Marka SSR (Simple Sequence Repeat)

Cited by 5 publications

References 19 publications

Similarity level of Nepenthes spp. based on the qualitative characters

Similarity level of Nepenthes spp. based on the qualitative characters

The Use of Molecular Markers to Analyze the Genetic Diversity of Indonesian Pepper (Capsicum spp.) Varieties Based on Anthracnose Resistance

Genetic Diversity Analysis and Development of DNA Fingerprints of 20 Indonesian Local Chili Pepper Varieties Based on SSR Markers

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