Kars Yöresi Koyunlarında Piyeten Olgularından Dichelobacter nodosus İzolasyonu ve PCR İle İdentifikasyonu

Abstract

In this study it was aimed to isolation and identification by PCR of specific agent from ovine footrot in Kars district and thus determination of prevalence of disease. To this end, 8,970 sheep belong to 10 different flocks were examined clinically, and in 1532 of these (17.07%) were found lameness for various reasons. Out of 247 (2.75%) of these cases were evaluated to be footrot suspect clinically. Bacteria were isolated in 205 (82.99%) of the 247 samples that were cultured in an anaerobic environment due to the suspicion of footrot. When Gram stains and microscopic investigation was carried out on these isolates, 195 of them (95.12%) were found to be Gram negative rod-type bacteria. These isolates were subjected by polymerase chain reaction (PCR) using Dichelobacter nodosus specific primer and amplicons (440bp) of expected weight in 153 (78.46%) of isolates were found. Considering of these findings, it was concluded that prevalence of disease is high in sheep in Kars district. Keywords