Kartogenin-Incorporated Thermogel Supports Stem Cells for Significant Cartilage Regeneration

Li, Xuezhou; Ding, Jianxun; Zhang, Zhengzheng; Yang, Modi; Yu, Jia-Kuo; Wang, Jincheng; Chang, Fei; Chen, Xuesi

doi:10.1021/acsami.5b12212

Cited by 165 publications

(146 citation statements)

References 46 publications

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“…Gel/KGN/BMSC implantation produced better histological cartilage repairs and mechanical properties, and greater extracellular matrix deposition than the implantation of thermogel only or BMSC‐loaded thermogel. Furthermore, more integrated and smoother repaired articular surfaces, more abundant characteristic glycosaminoglycans (GAGs) and Col II, and less degeneration of normal cartilage were observed in the KGN/BMSCs co‐loaded thermogel group in vivo . Shi et al devised a photo‐cross‐linked scaffold using KGN‐encapsulated nanoparticles integrated into an ultraviolet‐reactive, rapidly cross‐linkable scaffold for cartilage regeneration.…”

Section: Kgn and The Promotion Of Cartilage Healingmentioning

confidence: 99%

“…Furthermore, more integrated and smoother repaired articular surfaces, more abundant characteristic glycosaminoglycans (GAGs) and Col II, and less degeneration of normal cartilage were observed in the KGN/BMSCs co-loaded thermogel group in vivo. 7 can release kartogenin to 25 mM over 3 weeks in 100 mL PBS were injected into the knee joint at weeks 6 and 9 after OA induction. Normal articular cartilage from rats that did not undergo surgical procedures is also shown.…”

Section: Kgn and The Promotion Of Cartilage Healingmentioning

confidence: 99%

“…On the other hand, KGN is a stable small molecule and can be stored and transported at room temperature. These obvious superiorities as compared with peptide growth factors make KGN an attractive chondrogenic agent for cartilage regeneration …”

Section: Introductionmentioning

confidence: 99%

“…These obvious superiorities as compared with peptide growth factors make KGN an attractive chondrogenic agent for cartilage regeneration. 7 Several studies have explored the chondro-protective effects of KGN, 8,9 and our group has developed several formulations for the IA delivery of KGN, such as, KGNconjugated chitosan nano/microparticles, thermo-responsive polymeric nanospheres based on chitosan oligosaccharide conjugated pluronic F127, and hyluronate hydrogels containing polyethylene glycol (PEG/KGN) micelles. Although KGN was initially developed to treat OA, it has been used to treat other conditions, such as, disc and bone-tendon junction regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Application of kartogenin for musculoskeletal regeneration

2017

J Biomedical Materials Res

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Kartogenin (KGN) is a recently characterized small molecule that promotes the selective differentiation of mesenchymal stem cells into chondrocytes, and thus, KGN stimulates cartilage regeneration. KGN also possess chondro-protective effect. Furthermore, because it is a highly stable small molecule, KGN can be stored and transported at room temperature. These obvious superiorities over peptide growth factors make KGN a desirable chondrogenic agent for cartilage regeneration. Since its discovery, KGN has drawn much attention as a new chondrogenic drug for intraarticular (IA) treatment. Although it was originally developed with a focus on OA, it has been used to treat other conditions and to promote disc and bone-tendon junction regeneration. Our group has also developed several formulations for IA delivery of KGN including KGN-conjugated chitosan nano/microparticles, thermo-responsive polymeric nanospheres based on chitosan oligosaccharide conjugated pluronic F127, and hyluronate hydrogels containing polyethylene glycol (PEG/KGN) micelles. This review was undertaken to summarize current research on the action mechanism of KGN and the various formulations described in the literature that induce musculoskeletal regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1141-1148, 2018.

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Section: Kgn and The Promotion Of Cartilage Healingmentioning

confidence: 99%

Section: Kgn and The Promotion Of Cartilage Healingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of kartogenin for musculoskeletal regeneration

2017

J Biomedical Materials Res

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“…Recent studies have found that kartogenin (KGN), a small compound can promote the chondrogenic differentiation of human and mouse mesenchymal stem cells (MSCs) [13]. Several studies have used KGN to enhance wounded cartilage regeneration [14, 15]. We have shown that KGN-treated autologous tendon graft can enhance the wounded rat meniscus healing [16].…”

Section: Introductionmentioning

confidence: 99%

A novel full-reduced HMGB1 and kartogenin-containing bio-active scaffold for meniscus regeneration

Zhi-hong

Zou

et al. 2020

Preprint

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43 44 A novel bio-active scaffold for enhancing wounded meniscus healing has been developed by 45 combination of High mobility group box 1 protein (HMGB1) and kartogenin (KGN) with 46 alginate gel. The properties of the bio-active scaffold were also investigated using an in vitro cell 47 culture model and in vivo rat wounded meniscus healing model. This HMGB1-KGN-containing 48 bioactive scaffold released HMGB1 and KGN into wound area and kept high concentrations of 49 HMGB1 and KGN in the system for more than two weeks. This HMGB1-KGN-containing 50 bioactive scaffold also activated rat bone marrow stem cells (BMSCs) from G0 to GAlert stage and 51 promoted cell proliferation as evidenced by 5-bromo-2-deoxyuridine (BrdU) incorporation 52 testing. Our results also demonstrated that the HMGB1-KGN-containing bioactive scaffold 53 induced cell migration in vitro and recruited the cells to wound area to promote wounded rat 54 meniscus healing in vivo. 55 56 57 58 59 60 61 62 63 64 4 65 66 67The menisci are C-shaped fibrocartilage located between the femoral condyles and tibial plateau 68 to transmit the load across the joint space [1]. Menisci play an important role in joint 69 stabilization, proprioception, lubrication and protection of articular cartilage [2]. Such 70 environment and function lead to meniscus easily injury and damaged meniscus can cause the 71 progression of cartilage degeneration and result in osteoarthritis [3]. Clinical studies have found 72 that it is difficult to regenerate the meniscus due to the specific structural and functional 73 properties of the meniscus tissue [1]. 74 75Histology studies have shown that meniscus is a geometrically and biochemically complex tissue. 76Its outer 1/3 tissue is formed by collagen type I produced by fibroblast-like cells, while its inner 77 2/3 tissue is formed by collagen II and proteoglycan produced by chondrocyte-like cells [4]. 78 Vascularization in meniscus is of high relevance. The meniscus is fully vascularized during fetal 79 development though blood supply diminishes over time, receding to the peripheral 20-30% by 10 80 years of age [4], [1]. 81 82The injury in the avascular zone of the meniscus is generally more complex and broad, and is 83 often associated with a poor prognosis following repair [5]. Promoting the healing process in the 84 avascular zone of the meniscus is an ongoing challenge for both clinical medical doctors and 85 orthopedic researchers [6]. Recently, tissue engineering approaches that involve the use of adult 86 tissue-derived multi-potent stem cells and biomaterial scaffolds have gained increasing attention 87 5 for enhancing the wounded meniscus healing. However, the isolation and culture of stem cells 88 not only need the donor tissue obtained by additional surgical procedure but also take long time 89 and increase contamination risk. Therefore, recruiting stem cells to wound area to enhance 90 wound healing is still a new challenge for orthopedic researchers. 91 92 High mobility group box 1 protein (HMGB1) is a ubiquitously...

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Polymer Fiber Scaffolds for Bone and Cartilage Tissue Engineering

Zhang

Liu

Zeng

et al. 2019

Adv Funct Materials

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228

154

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Successful regeneration of weight-bearing bone defects and criticalsized cartilage defects remains a major challenge in clinical orthopedics. In the past decades, biodegradable polymer materials with biomimetic chemical and physical properties have been rapidly developed as ideal candidates for bone and cartilage tissue engineering scaffolds. Due to their unique advantages over other materials of high specific-surface areas, suitable mechanical strength, and tailorable characteristics, scaffolds made of polymer fibers have been increasingly used for the repair of bone and cartilage defects. This Review summarizes the preparation and compositions of polymer fibers, as well as their characteristics. More importantly, the applications of polymer fiber scaffolds with well-designed structures or unique properties in bone, cartilage, and osteochondral tissue engineering have been comprehensively highlighted. On the whole, such a comprehensive summary affords constructive suggestions for the development of polymer fiber scaffolds in bone and cartilage tissue engineering.

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Kartogenin-Incorporated Thermogel Supports Stem Cells for Significant Cartilage Regeneration

Cited by 165 publications

References 46 publications

Application of kartogenin for musculoskeletal regeneration

Application of kartogenin for musculoskeletal regeneration

A novel full-reduced HMGB1 and kartogenin-containing bio-active scaffold for meniscus regeneration

Polymer Fiber Scaffolds for Bone and Cartilage Tissue Engineering

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