Karyological and Molecular Genetic Differentiation of Fringed Sculpins of the Genus Porocottus Gill, 1859 (Cottidae: Myoxocephalinae)

Морева, И. Н.; Radchenko, O. A.; Petrovskaya, A. V.

doi:10.1134/s1063074019020081

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…Megalocottus platycephalus platycephalus (Pallas, 1814) vs. M. platycephalus taeniopterus (Kner, 1868) (Radchenko and Petrovskaya 2019), and at the species level, e.g. species of the genera Enophrys Swainson, 1839 and Porocottus Gill, 1859 (Moreva et al , 2019. This trend has also been observed in other fish families: Lycodes matsubarai Toyoshima, 1985 (Zoarcidae; Sakuma et al 2014), Bothrocara hollandi (Jordan et Hubbs, 1925) (Zoarcidae; Kodama et al 2008), and the southern and northern forms of Tribolodon hakonensis (Günther, 1877) (Cyprinidae; Ryazanova and Polyakova 2012).…”

Section: Discussionmentioning

confidence: 99%

“…These similarities, as well as NF (44 + 2), suggest that the metacentrics and submetacentrics in cytotypes II-V were formed through Robertsonian fusions. Karyological studies show that Robertsonian translocations are the main mechanism of structural changes in the chromosomes of marine sculpins of the family Cottidae (Vasilyev 1985;Terashima and Ida 1991;Moreva et al , 2019Radchenko et al 2020). Here we report the highest intraspecific variability in 2n (40 to 44) documented to date for this fish group (Arai 2011;Moreva et al , 2019Moreva 2020).…”

Section: Discussionmentioning

confidence: 99%

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Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

Морева¹,

Radchenko²,

Petrovskaya³

2021

CCG

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A molecular genetic and karyological study of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 was carried out on an extensive sample from a large area of the species’ range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism was found to be low. The haplotypes of the species formed three phylogenetic groups. The unique M. stelleri haplotype from the coast of Shikotan Island linked all the studied groups, indicating that it is likely ancestral. Robertsonian polymorphism was identified in the species. In all five cytotypes (I – 2n = 44, II – 2n = 43, III – 2n = 42, IV – 2n = 41, V – 2n = 40; NF = 44+2) were identified, all of which were present in the Sea of Japan. Only one (cytotype I) was found in the Sea of Okhotsk, which is probably the closest to the ancestral karyotype. The significant chromosomal polymorphism and the presence of common haplotypes in the studied samples indicate their recent origin from a common ancestor and/or relatively recent contacts within the range. The discrepancies between mtDNA and karyotypes in assigning the ancestral M. stelleri to the coastal waters off Shikotan Island (southern Kuril Islands) and the Sea of Okhotsk, respectively, can be explained by the different inheritance mechanisms and the rates of evolution of molecular genetic and karyological traits.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

Морева¹,

Radchenko²,

Petrovskaya³

2021

CCG

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“…The closest relationship was found between representatives of the genera Porocottus and Argyrocottus, whose karyotypes are similar in the number of chromosomal arms (42), and also by the presence of large and small M chromosomes and three pairs of large ST chromosomes (Table 2). The exception is P. allisi, which has only one pair of large ST chromosomes due to the involvement of the two other pairs in the Robertsonian translocation (Moreva et al, 2019). Species of Megalocottus and Myoxocephalus form another common group.…”

Section: Relationships Between the Genera Of The Subfamily Myoxocephalinaementioning

confidence: 99%

“…The fish symmetrized karyotype, consisting of only bi-armed elements, indicates completion of the evolutionary transformations of chromosome sets that occurred through centric fusions (Vasilyev, 1982). Robertsonian fusions are the main mechanism of both evolutionary changes in the karyotypes of Myoxocephalus and Porocottus and intraspecific variation of Myoxocephalus scorpius and Enophrys diceraus (Moreva et al, 2017(Moreva et al, , 2019Radchenko et al, 2020;Vasilyev, 1985). The polymorphism in the number of chromosomes found in Myoxocephalus scorpius and Enophrys diceraus may indicate the incompleteness of the evolution of their karyotypes (Moreva et al, 2017;Radchenko et al, 2020).…”

Section: Hypotheses Of Evolutionary Changes In Karyotypesmentioning

confidence: 99%

The subfamily Myoxocephalinae of cottid fishes (Cottidae): Genetic divergence and phylogenetic relationships

Radchenko

Морева

Petrovskaya

2021

Journal of Fish Biology

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A comprehensive study based on molecular marker and karyotype analyses has provided evidence for the monophyly of the subfamily Myoxocephalinae, which includes the genera Myoxocephalus, Megalocottus, Microcottus, Porocottus, Enophrys and Argyrocottus. In addition, the karyotype of the threadfoot sculpin Argyrocottus zanderi Herzenstein 1892 has been studied for the first time. Marker traits of karyotypes identified 13 species among six cottid genera. As the molecular genetic results confirmed, the subfamily is divided into two groups corresponding to Enophrys and Myoxocephalus. The molecular genetic data did not support the formation of tribes within the subfamily Myoxocephalinae, as proposed earlier based on morphological characters. Moreover, the genera Trichocottus and Taurocottus should be excluded from the Myoxocephalinae. The evolutionary transformations of karyotypes in cottid fish tended towards a reduction in the number of chromosomes and chromosome arms.

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The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

Turanov

Rutenko

2020

Preprint

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The taxonomy of fish in the northeast Pacific area has been recently revised using molecular genetic methods, including the development of a reference library of DNA fragments for species identification. Such libraries are the basis for the development of non-invasive, high-throughput methods for monitoring biodiversity using environmental DNA (eDNA). In order to validate this approach, we used a water eDNA metabarcoding technique based on 12S rRNA and COI mitochondrial fragments and assessed the composition of the twenty northeast Pacific fish species held in a fish tank at the Primorsky Aquarium (Vladivostok, Russia). Only the 12S fragment revealed data on fish-related operational taxonomic units (OTUs). Approximately 68% of the reads were classified into two species of the genus Oncorhynchus, whose shredded fillet is used for feeding. According to the taxonomic identification for the rest of the reads, 8 out of 20 fish species in the tank (40%) were identified unambiguously, while two species could not be identified. Ten taxa can be considered conditionally identifiable since they might be concealed behind a conflicting taxonomic identification at the genus or family level. In this case, an improvement of the reference library would provide resolution. We detected contamination, which may be related to both intra-laboratory contaminants occurring during DNA extraction and water intake supplying the fish tank.

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Karyological and Molecular Genetic Differentiation of Fringed Sculpins of the Genus Porocottus Gill, 1859 (Cottidae: Myoxocephalinae)

Cited by 3 publications

References 11 publications

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

The subfamily Myoxocephalinae of cottid fishes (Cottidae): Genetic divergence and phylogenetic relationships

The composition of northeast pacific fishes in a fish tank examined by eDNA metabarcoding

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