Karyomorphological Study in &lt;i&gt;Ledebouria botryoides&lt;/i&gt; (Asparagaceae)

Deshmukh, Pradip V.; Yadav, Shrirang R.; Lekhak, Manoj M.

doi:10.1508/cytologia.88.15

Cited by 3 publications

(2 citation statements)

References 18 publications

(33 reference statements)

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“…We measured chromosome length and estimated the relative length (RL) and centromeric index (CI) ( Supplementary Table S1 ). All data were used in karyotyping and diagramming [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)

Xu,

Li,

et al. 2024

Genes

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The sex chromosomes of skinks are usually poorly differentiated and hardly distinguished by cytogenetic methods. Therefore, identifying sex chromosomes in species lacking easily recognizable heteromorphic sex chromosomes is necessary to fully understand sex chromosome diversity. In this paper, we employed cytogenetics, sex quantification of genes, and transcriptomic approaches to characterize the sex chromosomes in Plestiodon elegans. Cytogenetic examination of metaphases revealed a diploid number of 2n = 26, consisting of 12 macrochromosomes and 14 microchromosomes, with no significant heteromorphic chromosome pairs, speculating that the sex chromosomes may be homomorphic or poorly differentiated. The results of the sex quantification of genes showed that Calumenin (calu), COPI coat complex subunit γ 2 (copg2), and Smoothened (smo) were at half the dose in males as in females, suggesting that they are on the X chromosome. Transcriptomic data analysis from the gonads yielded the excess expression male-specific genes (n = 16), in which five PCR molecular markers were developed. Restricting the observed heterozygosity to males suggests the presence of homomorphic sex chromosomes in P. elegans, XX/XY. This is the first breakthrough in the study of the sex chromosomes of Plestiodon.

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“…We measured chromosome length and estimated the relative length (RL) and centromeric index (CI) ( Supplementary Table S1 ). All data were used in karyotyping and diagramming [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)

Xu,

Li,

et al. 2024

Genes

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“…CI was estimated to classify the types of chromosomes (Chooseangjaew et al 2017). All data were used in karyotyping and diagramming (Deshmukh et al 2023).…”

Section: Chromosome Checksmentioning

confidence: 99%

First karyological analysis of <i>Plestiodon capito</i> (Squamata: Scincidae)

Xu,

Zhu

2024

CYTOLOGIA

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New Cytogenetic Data for the Neottieae Tribe (Orchidaceae) in the Mediterranean Region

Turco,

Wagensommer,

Albano

et al. 2024

Plants

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This work presents a summary of cytogenetic data, including new information, on several species within the tribe Neottieae, with an update of the karyotype for 23 species belonging to the genera Cephalanthera, Limodorum, Epipactis, and Neottia (including Listera). Each of these four genera also presents distinctive chromosomal features, such as bimodal karyotypes. Our research includes insights into the distribution of constitutive heterochromatin, measured using C-banding and, in some cases, specific fluorochromes for the detection of A-T- and G-C-rich DNA. In the Epipactis group, it is noteworthy that when using the Giemsa banding technique, certain species (e.g., E. placentina, E. meridionalis) with a chromosome number of 2n = 38 were observed to exhibit a conspicuous wide band of constitutive heterochromatin on the long arm of the third pair in a subcentromeric position, resembling what has been observed in E. helleborine. These differences also have the potential to contribute to the diversification of these species. Based on the karyological results obtained, a hypothesis regarding the origin of certain species within the E. helleborine group is proposed. Additionally, karyological analyses conducted on a specimen of E. microphylla revealed chromosome counts ranging from 36 to 40. Somatic metaphases exhibited evident structural alterations in certain chromosomes, showing rearrangements probably caused by translocation phenomena. Based on the data obtained from the species within the studied genera, it is conceivable that variations in chromosomes, both structurally and in the distribution of constitutive heterochromatin, exert a significant influence on the evolution of the karyotype. Moreover, in many entities belonging to the Neottieae tribe, these processes may also contribute to the diversification of the phenotype in some instances.

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Karyomorphological Study in <i>Ledebouria botryoides</i> (Asparagaceae)

Cited by 3 publications

References 18 publications

The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)

The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)

First karyological analysis of <i>Plestiodon capito</i> (Squamata: Scincidae)

New Cytogenetic Data for the Neottieae Tribe (Orchidaceae) in the Mediterranean Region

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