Karyotype Analysis and Giema C-Banding Patterns in <i>Thinopyron elongatum</i>

Abstract

Giemsa C-banding patterns and karyotype of chromosomes were analyzed in the root-tip cells of diploid Thinopyron elongatum. A modified seed germinating method was developed for obtaining the C-banding patterns in diploid Thinopyron elonggatum. The results of The C-banding analysis showed the significant differences among the seven pairs of chromosomes in diploid Thinopyron elonggatum. The intensive C-bands were stained steadily on the intercalary, terminal, subcentromeric and centromeric regions of Chromosomes. We found that there are three pairs of metacentric chromosomes and four pairs of submetacentric chromosomes in diploid Thinopyron elonggatum. The karyotype formula is 2n=2x=14=6m+8sm. Present results provided a basic cytological data and will be useful for the further studies in Thinopyron elongatum.

A FISH Karyotype to Study Chromosome Polymorphisms for the Elytrigia elongata E Genome

“…The relative number and position of 5S and 45S ribosomal DNA sites and the hybridization pattern of the pSc119.2 and pAs1 clones were used to analyze the T. ponticum genome [Brasileiro-Vidal et al, 2003]. Because the diploid E genome does not C-band well, after the early C-banding analysis of several diploid Agropyron species, the karyotype analysis and Giemsa C-banding of E. elongata chromosomes were very recently reported by Min [2011]. However, a detailed description of the FISH pattern of the individual chromosomes of E. elongata is still missing, and this makes it difficult to analyze the progenies of intergeneric hybrids or identify the incorporated alien DNA segments in a wheat background.…”