Karyotype analysis and physical mapping of the 5S and 45S rDNA genes in Tigridia pavonia var. Dulce (Iridaceae)

Arroyo-Martínez, Hugo Abelardo; Arzate-Fernández, Amaury M.; Barba-González, R.; Piña-Escutia, José Luis

doi:10.1080/00087114.2017.1382302

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…Barreto dos Reis et al (2015) reported that the hybrid Pennisetum purpureum X P. glaucum showed DNA loss in relation to their parents, pointing out that it could be due to genomic alteration of the hybridization. In the present study similar results were found since chromosome length range of Penélope was different from those reported previously for Trinidad and Dulce (Arroyo-Martínez et al, 2018). Our results are also coincided with Riddle and Birchler (2003) who mentioned that union of two genomes in a single nucleus can alter the number and distribution of DNA sequences, which could cause genetic and epigenetic reorganizations, resulting in intergenomic conflicts and DNA loss.…”

Section: Karyotype Analysessupporting

confidence: 77%

“…In the present study similar results were found since Trinidad showed six hybridization sites for both genes (chromosomal pairs 1, 10 and 12) two of them were found in two of the large chromosomes and the rest in the small ones (Figure 4ac). Interestingly Penélope also showed two hybridization sites of both genes in two of the large chromosomes and the rest in the small ones (Figure 4bd), contrary to reported for Dulce where none hybridization site corresponded to large chromosomes (Arroyo-Martínez et al, 2018), this suggest that Trinidad and Penélope could share similar genetic accommodation of their DNA sequences. Our results also coincide with reported by Piña-Escutia et al (2010b) who observed a narrow genetic distance between Trinidad and Penélope when these were evaluated with RAPD markers.…”

Section: Physical Mapping Of 5s and 45s Rdna Genescontrasting

confidence: 67%

“…As it is known, hybridization may enhance the colonizing behavior of certain species (López-Caamal and Tovar-Sánchez 2014) allowing that parental species may coexist through formation of a stable hybrid zone (Hall 2016). These reports strengthen the idea that Penélope could be a natural hybrid between Trinidad and Dulce varieties, especially because Penélope showed the same number of B chromosomes reported for Dulce (Arroyo-Martínez et al, 2018). Likewise, Penélope showed intermediate values of LGT in comparison with the other two varieties (Table 1) however, a higher similarity with Dulce (p ≤ 0.05) was observed.…”

Section: Karyotype Analysessupporting

confidence: 52%

“…In this regard, of the nine varieties of T. pavonia registered in 2016 in the Catálogo Nacional de Variedades Vegetales, it is considered that at least two of them could be natural hybrids, although so far no research has been reported that confirms it. Thus, it is believed that Penélope variety is one of them, whose parents could be Trinidad and Dulce varieties, the latter showing three B chromosomes in their karyotype (Arroyo-Martínez et al, 2018). It is noteworthy pointed out that Penélope have showed intermediate features for color, height plant and fertility percentage in relation to Trinidad and Dulce varieties and the three share the same geographic location.…”

Section: Introductionmentioning

confidence: 99%

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CYTOGENETIC RELATIONSHIPS IN THREE VARIETIES OF Tigridia pavonia (L.f.) DC

Arroyo-Martínez

Arzate-Fernández

Barba-González³

et al. 2020

Trop Subtrop Agroecosyst

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Background: Tigridia pavonia (L.f.) DC is a wild species with great ornamental value, of which nine plant varieties are known. Within the evolutionary process of this species, Penélope has been considered a natural hybrid, product of the cross between the varieties Trinidad and Dulce. Objective: In the present study, the cytogenetic relationships among Trinidad, Dulce and Penélope were analyzed. Methodology: The karyotype of the varieties Trinidad and Penélope was determined through classic cytogenetics and the physical mapping of the genes 5s and 45s rDNA through Fluorescent In Situ Hybridization. Results: The results showed for the first time the karyotype and the physical mapping of the genes 5s and 45s rDNA in the varieties Trinidad and Penélope. Implications: The information generated can be the basis for future evolutionary analyzes, and / or breeding programs in the species. Conclusion: A higher cytogenetic similarity of Penélope with Trinidad and Dulce has been revealed, suggesting that the latter may be the parents.

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Section: Karyotype Analysessupporting

confidence: 77%

Section: Physical Mapping Of 5s and 45s Rdna Genescontrasting

confidence: 67%

Section: Karyotype Analysessupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

CYTOGENETIC RELATIONSHIPS IN THREE VARIETIES OF Tigridia pavonia (L.f.) DC

Arroyo-Martínez

Arzate-Fernández

Barba-González³

et al. 2020

Trop Subtrop Agroecosyst

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“…Tigridia pavonia (L.f) DC also known as "Oceloxochitl" or "Tiger flower" is a Mexican native ornamental plant that displays great variability in color and beauty of its ephemeral flowers (Piña-Escutia et al, 2013;Arzate-Fernández et al, 2015;Arroyo-Martínez et al, 2017). Although their flower longevity consists of one day, flower production continues for up eight weeks, being an excellent outdoor plant.…”

Section: Introductionmentioning

confidence: 99%

CHARACTERIZATION OF THE SENESCENCE-ASSOCIATED PROTEASES OF THE “TIGER FLOWER” (Tigridia pavonia) L.F. DC

Escutia

Mondragón

Fernández

2021

Trop Subtrop Agroecosyst

Self Cite

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Background: Tigridia pavonia is a Mexican native ornamental plant that displays great beauty and color of its flower. However, its quick senescence limits its use as cut flower, since these open in the morning and close in the afternoon of the same day exhibiting wilting of tepals. Objective: In this study flower senescence process of T. pavonia was analyzed. Methodology: The expression of several proteases during senescence of tepals, ovaries and anthers was measured by SDS-PAGE and their identification was through mass spectrometry. Results: Five proteases of apparent molecular weight of 130, 90, 65, 35 and 30 kDa were expressed during flower senescence process. The 65 kDa protease was identified as cysteine-protease and had a good homology score with early responsive to dehydration protein (ERD) besides their expression coincided with the onset of visible symptoms of tepals senescence. Implications: The preliminar sequence found belonging to ERD gen indicates that it could act as the main transcription factor integrating signaling pathways that trigger flower senescence in T. pavonia. Conclusion: Flower senescence of T. pavonia is mainly associated with the expression of cysteine-proteases. One of them is possibly associated with programmed cell death in tepals.

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Can ploidy levels explain the variation of Herbertia lahue (Iridaceae)?

Stiehl-Alves,

Vieira,

Trevelin

et al. 2023

Genet. Mol. Biol.

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Polyploidy is often related with phenotypic variation, as observed in Herbertia lahue , a geophyte species. This study examined the H. lahue polyploid series and departure in cytogenetic, morphometric, and pollen data. Diploids (2 n =2 x =14) present bimodal karyotype with two long and five short chromosome pairs, while hexaploids (2 n =6 x =42) and octoploids (2 n =8 x =56) present a gradual decrease in chromosome size. All cytotypes have CMA + /DAPI - bands co-localized with 18S rDNA sites in the satellite region (no DAPI + bands in any cytotype). Unlike diploids and octoploids, 5S rDNA interstitial sites in hexaploids are not in a syntenic position with 18S rDNA sites. Genome size is effective as an indirect predictor of the cytotypes since 2C-values increased according to ploidy level. The reduction in the number of the rDNA sites in polyploids associated with their lower 1C x -values compared to diploids may suggest a genome downsizing process. Morphometric analysis revealed significant differences among cytotypes, and discriminant analysis identified three morphometric groupings corresponding to the cytotypes. The phenotypic variation observed in pollen grains, bulbs, and ovary characters suggested the gigas effect. Concluding, remarkable differentiation was observed at both genomic and phenotypic characters in all the cytotypes analyzed, suggesting a possible ongoing speciation process in H. lahue .

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Karyotype analysis and physical mapping of the 5S and 45S rDNA genes in Tigridia pavonia var. Dulce (Iridaceae)

Cited by 4 publications

References 31 publications

CYTOGENETIC RELATIONSHIPS IN THREE VARIETIES OF Tigridia pavonia (L.f.) DC

CYTOGENETIC RELATIONSHIPS IN THREE VARIETIES OF Tigridia pavonia (L.f.) DC

CHARACTERIZATION OF THE SENESCENCE-ASSOCIATED PROTEASES OF THE “TIGER FLOWER” (Tigridia pavonia) L.F. DC

Can ploidy levels explain the variation of Herbertia lahue (Iridaceae)?

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