Tissue culture and subsequent plant regeneration has now been developed for several spe cies and the nature of genetic and chromosomal aberrations in regenerated plants have been demonstrated (Reisch 1983). In addition to gross changes in chromosome numbers , more cryptic chromosome rearrangements may be responsible for genetic variation in cultured cells and regenerated plants (Larkin and Scowcroft 1981) . Chromosome breakage and reunion, multicentrics and translocations have been observed in the plants derived from cultures of barley (Orton 1980). Cummings et al. (1976 and McCoy et al. (1982) observed hetero morphic pairs (deletions or translocations) in oat somaclones. Heteromorphic pairs were also found in maize somaclones (Green et al., 1977). Cytogenetic changes have also been observed in regenerated plants of Alfa alfa (McCoy and Bingham 1977) , Celery (Bowers and Orton 1982), Lycopersicum (Ancora and Sree Ramlulu 1981), wheat (Bennici and D'Amato 1978), potato (Sree Ramlulu et al. 1983) and Brassica napus (Newell et al. 1984) .In Liliaceae, although protocols for plant regeneration are available for over 26 species (Flick et al. 1983) data on cytogenetic analysis are rather meagre (Novak 1980, Krikorian et al. 1981and Sheridan 1975. In the present project on tissue culture of medicinally important plants of family Liliaceae, a large number of plants were regenerated from bulb derived calli of Urginea indica (Jha et al. 1984). This plant is of special significance for bufadienolide content (Jha andSen 1981, 1983a). The present investigation is aimed to examine the chromosomal variations, if any, occurring in callus regenerated plants.