Karyotype Analysis of Interspecific Rat/Mouse Somatic Cell Hybrids by Reverse Chromosome Painting

Helou, Khalil; Levan, Göran; Klinga-Levan, Karin

doi:10.1111/j.1601-5223.1998.t01-1-00091.x

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Twenty-one of these primary tumor cell cultures were used in the present study (Table 1), 10 derived from crosses with the SPRD background and 11 with the BN background. A rat embryo fibroblasts (REF) cell culture was used as normal control [23]. All animal experiments was approved by the local ethical committee (Institute of Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Germany).…”

Section: Methodsmentioning

confidence: 99%

SKY analysis revealed recurrent numerical and structural chromosome changes in BDII rat endometrial carcinomas

et al. 2011

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BackgroundGenomic alterations are common features of cancer cells, and some of these changes are proven to be neoplastic-specific. Such alterations may serve as valuable tools for diagnosis and classification of tumors, prediction of clinical outcome, disease monitoring, and choice of therapy as well as for providing clues to the location of crucial cancer-related genes.Endometrial carcinoma (EC) is the most frequently diagnosed malignancy of the female genital tract, ranking fourth among all invasive tumors affecting women. Cytogenetic studies of human ECs have not produced very conclusive data, since many of these studies are based on karyotyping of limited number of cases and no really specific karyotypic changes have yet been identified. As the majority of the genes are conserved among mammals, the use of inbred animal model systems may serve as a tool for identification of underlying genes and pathways involved in tumorigenesis in humans. In the present work we used spectral karyotyping (SKY) to identify cancer-related aberrations in a well-characterized experimental model for spontaneous endometrial carcinoma in the BDII rat tumor model.ResultsAnalysis of 21 experimental ECs revealed specific nonrandom numerical and structural chromosomal changes. The most recurrent numerical alterations were gains in rat chromosome 4 (RNO4) and losses in RNO15. The most commonly structural changes were mainly in form of chromosomal translocations and were detected in RNO3, RNO6, RNO10, RNO11, RNO12, and RNO20. Unbalanced chromosomal translocations involving RNO3p was the most commonly observed structural changes in this material followed by RNO11p and RNO10 translocations.ConclusionThe non-random nature of these events, as documented by their high frequencies of incidence, is suggesting for dynamic selection of these changes during experimental EC tumorigenesis and therefore for their potential contribution into development of this malignancy. Comparative molecular analysis of the identified genetic changes in this tumor model with those reported in the human ECs may provide new insights into underlying genetic changes involved in EC development and tumorigenesis.

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Section: Methodsmentioning

confidence: 99%

SKY analysis revealed recurrent numerical and structural chromosome changes in BDII rat endometrial carcinomas

et al. 2011

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“…Moreover, detection of small chromosomal rearrangement, such as translocations and insertions, can be extremely difficult with G-banding methods. To reduce these problems, fluorescence in situ hybridization (FiSH) techniques with genomic DNa are used to discriminate parental chromosomes in cell hybrids such as mouse-hamster [1], mouse-human [5], canine-mouse [6], and rat-mouse [3]. However, these techniques are conducted by the discrimination of either species chromosome to a chromosome spread with hybrid cells.…”

Section: Introductionmentioning

confidence: 99%

Use of Genomic <i>In Situ</i> Hybridization to Identify Chromosomes in Gerbil-Mouse Heterohybridomas

et al. 2013

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Heterohybridomas, created to secrete monoclonal antibodies, are generally unstable over long-term culture because of chromosome elimination during culture. To evaluate the karyotype of heterohybridomas, we used simultaneous genomic in situ hybridization (GISH), which can distinguish unambiguously between parental genomes in allopolyploid species. Using GISH, we discriminated gerbil and mouse chromosomes in heterohybridomas with high sensitivity. The GISH technique will allow evaluation of the stability and crossability of heterohybridomas made from the fusion of mouse cells with those of related species.

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Karyotype Analysis of Interspecific Rat/Mouse Somatic Cell Hybrids by Reverse Chromosome Painting

Cited by 2 publications

References 10 publications

SKY analysis revealed recurrent numerical and structural chromosome changes in BDII rat endometrial carcinomas

SKY analysis revealed recurrent numerical and structural chromosome changes in BDII rat endometrial carcinomas

Use of Genomic <i>In Situ</i> Hybridization to Identify Chromosomes in Gerbil-Mouse Heterohybridomas

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