Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand

Saensouk, Surapon; Saensouk, Piyaporn

doi:10.13057/biodiv/d220844

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…The diploid chromosome numbers of each specimen in this study were counted from 20 cells. For the arrangement of the karyotypes, the following parameters, such as average length of the short arm (Ls), average length of the long arm (Ll), length of each chromosome (LT), average measurement of relative length (RL), chromosome index (CI), standard deviation (SD) of RL and CI from metaphase chromosomes, were calculated by the methods of Saensouk et al (2019), Senavongse et al (2018;2020), Saensouk and Saensouk (2021a) and Saensouk and Saensouk (2021b).…”

Section: Methodsmentioning

confidence: 99%

Karyotype analysis from four species of edible plants in northeastern Thailand

Saensouk

Saen-in

2021

Biodiversitas

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Karyotype analysis from four species of edible plants, namely one species from family Cannaceae (Canna indicaL.), one species including 2 variants from family Araceae (Colocasia esculenta(L.) Schott, green taro and black taro) and two species from family Apiaceae (Eryngium foetidum L. and Centella asiatica (L.) Urb.) in Maha Sarakham province, northeastern Thailand were determined from root tips. The results showed that the chromosome numbers and karyotype formulae were 2n= 28 = 14m + 10sm + 4st (2sat) with NF = 56 for green taro, 2n= 42 = 20m + 20sm + 2st (3sat) with NF = 84 for black taro, 2n = 18 = 18sm with NF = 36 for Centella asiatica (L.) Urb., 2n = 16 = 2m +14 sm with NF = 32 for Eryngium foetidum L., 2n= 27 = 2m + 25sm with NF = 54 for Canna indica L. Both taros had differences in the chromosome numbers and karyotype formulae. The chromosomes of both taros were the asymmetrical karyotype. While, the chromosome structure of both taros, including the number of metacentric, submetacentric and subtelocentric as well as the position of satellite-chromosomes, were found to be different, which indicated inter-and intra-specific variations in this species. Therefore, the chromosome number, karyotype formula, chromosome structure, position of satellite-chromosomes and idiogram can be used for classification of both taros in this study. Moreover, idiograms of both taros were reported for the first time. The karyotype, NF, RL, CI and ideogram of Centella asiatica(L.) Urb., Eryngium foetidum L. and Canna indica L. were reported for the first time.

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Section: Methodsmentioning

confidence: 99%

Karyotype analysis from four species of edible plants in northeastern Thailand

Saensouk

Saen-in

2021

Biodiversitas

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“…The chromosome number, chromosome length range, haploid chromosome length, arm ratio, relative length, and karyotype formula were determined from 10 metaphase cells in each species. For the arrangement of the chromosomes in the karyotypes, the parameters for the average length of the short arm (Ls), the average length of the long arm (Ll), length of each chromosome (LT), average relative length (RL), chromosome index (CI), and standard deviations (SD) of RL and CI from the metaphase chromosomes were calculated according to Senavongse et al (2018Senavongse et al ( , 2020, Saensouk et al (2019, 2021b.…”

Section: Mitotic Karyotype and Ideogram Analysismentioning

confidence: 99%

Cytogenetic study of five species of medicinal plants from Maha Sarakham Province, Thailand

2022

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Abstract. Saensouk S, Saen-in N, Saensouk P. 2022. Cytogenetic study of five species of medicinal plants from Maha Sarakham Province, Thailand. Biodiversitas 23: 3593-3603. The cytogenetic study of five species of medicinal plants, namely Butomopsis latifolia (D. Don) Kunth, Iris domestica (L.) Goldblatt & Mabb., Limnocharis flava (L.) Buchenau, Murdannia loriformis (Hassk.) R.S. Rao & Kammathy, and Pontederia hastata L. from Maha Sarakham Province, northeastern Thailand was conducted. This study aimed to investigate the chromosome numbers, fundamental number (NF), karyotype forms, and perform ideogram analysis of the five species. The cytogenetic characteristics, including the chromosome number, karyology (fundamental number (NF), karyotype formula), symmetrical karyotype, chromosome size, relative length (RL), centromeric indexes (CI), and ideograms of B. latifolia (D. Don) Kunthwere studied for the first time. The chromosome numbers of five medicinal plant species were reported as 2n = 14 (B. latifolia (D. Don) Kunth)- 80 (I. domestica (L.) Goldblatt & Mabb.). Moreover, the NF had 28 (B. latifolia (D. Don) Kunth) - 80 (Iris domestica). Two species (M. loriformis(Hassk.) R.S. Rao & Kammathyand P. hastata L.) were recognized as having symmetrical karyotypes. Three species, namely B. latifolia (D. Don) Kunth, Iris domestica, and L. flava(L.) Buchenau were reported as having asymmetrical karyotypes. Chromosomes of large size (L), chromosomes of medium size (M), and chromosomes of small size (S) were observed. Ideograms of five species were provided in this study. The NF, RL, CI, chromosome size, and ideograms of all species were reported for the first time in this study. The karyotype formulae of the five species, except the karyotype formula of P. hastata L., differed from those previously studied. The cytogenetic data of the five species in this study can be used for identification.

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“…So far, karyotype analysis has been wildly used for species identification, cytotaxonomy study, and molecular phylogenetic analysis in plant and other organisms [ 15 , 19 , 20 , 21 , 22 , 23 ]. Although the morphology-based karyotype analysis is simple and easy to operate, it has some limitations.…”

Section: Introductionmentioning

confidence: 99%

Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids

Cui

Chen

Zhao

et al. 2022

IJMS

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Itoh hybrids are intersectional hybrids in Paeonia L. with sect. Moutan and sect. Paeonia as paternal and maternal parents, respectively. Therefore, these hybrids have herbaceous stems with improved ornamental value introduced by the paternal parent. Although both of their parents are diploids, Itoh hybrids are triploids. Moreover, the parental origin of their chromosomes has not been extensively studied. This study systematically analyzed the genome size, ploidy, and karyotype of Itoh hybrids and compared them with their parental taxa. Although the monoploid genome size of Itoh hybrids was different, it was not significantly different from that of the parents. However, the size of varieties in the two parental taxa was significantly different from the wild species, probably due to genome rearrangements caused by artificial selection. Further karyotype analysis, correlation analysis, and hierarchical clustering could not identify the parental origin of chromosomes in Itoh hybrids. Verification through genomic and fluorescence in situ hybridization (GISH and FISH) suggested that for the three sets of chromosomes in Itoh hybrids, two were from the paternal parent, and one was from the maternal parent. One of the first two sets was from wild species, and the other from a cultivated variety. GISH could not label the chromosomes of cultivated peonies from the sect. Moutan, probably due to the huge and complex genomes compared with the wild species. Meanwhile, 5S rDNA-based FISH was first applied in Paeonia, which may be used for ploidy assessment. This work may give insights into the utilization of Itoh hybrid resources.

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Karyotype analysis of three species of Allium (Amaryllidaceae) from Thailand

Cited by 3 publications

References 24 publications

Karyotype analysis from four species of edible plants in northeastern Thailand

Karyotype analysis from four species of edible plants in northeastern Thailand

Cytogenetic study of five species of medicinal plants from Maha Sarakham Province, Thailand

Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids

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