Karyotype and FISH analysis of a newly established cell line derived from a human bladder carcinoma

Perissel, B; Benkhalifa, Moncef; Taillandier, J.; Dastugue, Nicole; Haus, Olga; Charbonné, F; Giraud, B; Malet, P

doi:10.1016/0165-4608(93)90160-n

Cited by 8 publications

(1 citation statement)

References 15 publications

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“…RT4 (15), T24 (16), TCCSUP (17) and J82 (18) were obtained from American Type Culture Collection (Rockville, USA) and RT112 (19) from Deutsches Krebsforschungszentrum TumorBank. CHA89 (20) was kindly provided by Dr. Bernard Perissel (Faculté de médecine, Clermont-Ferrand, France). All cell lines were maintained in the recommended growth media, as described (21).…”

Section: Cell Culturementioning

confidence: 99%

Fibrinogen mediates bladder cancer cell migration in an ICAM-1-dependent pathway

Roche

Pasquier²,

Rambeaud³

et al. 2003

Thromb Haemost

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SummaryFibrinogen (fg), present in tumor matrices, has been demonstrated to be determinant in metastatic potential. We have recently shown that fg/ICAM-1 interactions are involved in leukocyte migration across endothelial cell monolayers. Using bladder transitional cell carcinoma as a model, we will show in this study that bladder high grade tumor cell lines express ICAM-1, and that this expression induces an fg-mediated migration. This phenomenon was dependent on ICAM-1/fg interaction as well as RhoA activity. ICAM-1 was concentrated in focal adhesion plaques when tumor cells were allowed to adhere on immobilized fg, suggesting a role in cell migration. The addition of fg induced a 3- to 6-fold enhancement of bladder tumor cell migration through HUVEC monolayers. This process was inhibited by an anti-ICAM-1 antibody blocking fg binding, demonstrating that ICAM-1/fg interaction was involved in the extravasation process. Finally, immunohistological studies revealed that the expression of ICAM-1 was closely associated with an infiltrative histological phenotype.Part of this paper was originally presented as part of the joint meetings of the 16th International Congress of the International Society of Fibrinolysis and Proteolysis (ISFP) and the 17th International Fibrinogen Workshop of the International Fibrinogen Research Society (IFRS) held in Munich, Germany, September, 2002.

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Section: Cell Culturementioning

confidence: 99%

Fibrinogen mediates bladder cancer cell migration in an ICAM-1-dependent pathway

Roche

Pasquier²,

Rambeaud³

et al. 2003

Thromb Haemost

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Bladder Cancer

Knuechel

Masters

Human Cell Culture

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Normal karyotype mosaicism in adult AML patients with adverse-risk and undefined karyotype: preliminary report of treatment outcomes after hematopoietic stem cell transplantation

et al. 2013

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Karyotype analysis in acute myeloid leukemia (AML) is one of the powerful prognostic factors for complete remission (CR), relapse, and overall survival (OS). Cytogenetic mosaicism is considered to be one of the important characteristics in expression of phenotypic manifestations. However, it has not come into focus due to emerging molecular biological approaches and the results of a number of mutation studies. Clinical correlates and prognostic relevance of mosaicism were evaluated in 163 AML patients [adverse-risk karyotypes (n = 72) and undefined karyotypes (n = 91)]. All patients were treated by induction and consolidation chemotherapies and finally went on hematopoietic stem cell transplantations (HSCT). Patients were divided into two subgroups, either with or without normal karyotype (NK) mosaicism. Seventy patients exhibited NK mosaicism and 93 did not. There were no significant differences in age, gender, chemotherapy cycles to achieve CR, HSCT donor type, source or intensity properties between the two subgroups. We found that NK mosaicism remaining in adverse-risk and undefined karyotype at diagnosis significantly correlates with better OS (p = 0.001) and lower CIR (p = 0.021) rate after HSCT. Our data show that the poor prognostic properties of unfavorable risk karyotype can be overcome to a great extent by allogeneic HSCT and chronic GVHD, especially in the subgroup with NK mosaicism. Cytogenetic mosaicism at initial diagnosis can be an influential factor for survival outcomes, even after HSCT.

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Karyotype and FISH analysis of a newly established cell line derived from a human bladder carcinoma

Cited by 8 publications

References 15 publications

Fibrinogen mediates bladder cancer cell migration in an ICAM-1-dependent pathway

Fibrinogen mediates bladder cancer cell migration in an ICAM-1-dependent pathway

Bladder Cancer

Normal karyotype mosaicism in adult AML patients with adverse-risk and undefined karyotype: preliminary report of treatment outcomes after hematopoietic stem cell transplantation

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