Karyotype and genome size variation in Delphinium subg. Anthriscifolium (Ranunculaceae)

Abstract

Five taxa of Delphinium subg. Anthriscifolium have been karyologically studied through chromosome counting, chromosomal measurement, and karyotype symmetry. Each taxon that we investigated has a basic chromosome number of x = 8, D. anthriscifolium var. savatieri, D. anthriscifolium var. majus, D. ecalcaratum, and D. callichromum were diploid with 2n = 16, while D. anthriscifolium var. anthriscifolium was tetraploid with 2n = 32. Monoploid chromosome sets of the investigated diploid taxa contained 1 metacentric chromosome, 3 submetacentric chromosomes, and 4 subtelocentric chromosomes. Higher interchromosomal asymmetry (CVCL) was present in D. ecalcaratum and D. callichromum than in other taxa. The highest levels of intrachromosomal asymmetry (MCA) and heterogeneity in centromere position (CVCI) were found in D. anthriscifolium var. majus. Diploid and tetraploid genome sizes varied by 3.02–3.92 pg and 6.04–6.60 pg, respectively. Karyotype and genome size of D. anthriscifolium var. savatieri, D. anthriscifolium var. majus, D. callichromum, and D. ecalcaratum were reported for the first time. Finally, based on cytological and morphological data, the classification of Delphinium anthriscifolium was revised.

Chromosome characterization and genome size in <i>Nigella</i> and <i>Garidella</i> species (Ranunculaceae) with their taxonomic implications

“…Flow cytometry (FCM) is a quick and valuable method to estimate genome size and has become a frequently used technique in plant studies (Dolezel and Bartos 2005). So far, several reports on genome sizes indicating their role in phylogenetic relationships have been reported for Delphinium (Luo et al 2023), Hepatica (Mabuchi et al 2005), and Ranunculus (Cires et al 2009). However, the Plants DNA C-value Database (https://cvalues.science.kew.org) contains the genome size of N. sativa and N. damascena only, none of the species in Garidella have been reported in terms of genome size.…”