Karyotype characterization and ZZ/ZW sex chromosome heteromorphism in two species of the catfish genus Ancistrus Kner, 1854 (Siluriformes: Loricariidae) from the Amazon basin

Oliveira, Renildo Ribeiro de; Feldberg, Eliana; Anjos, Maeda Batista dos; Zuanon, Jansen

doi:10.1590/s1679-62252007000300010

Cited by 41 publications

(46 citation statements)

References 30 publications

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“…Single NORs at interstitial position are putatively a primitive feature within Ancistrini, as proposed by Artoni & Bertollo (2001), Alves et al (2003), Mariotto et al (2004), Mariotto & Miyazawa (2006), de Oliveira et al (2006de Oliveira et al ( , 2007, Mariotto et al (2009), Mariotto et al (2011) and the present study. Nevertheless, NORs located at terminal position of chromosomes are observed in some species, as verified in Ancistrus n.sp.…”

Section: Discussionsupporting

confidence: 81%

“…Since species bearing multiple sex chromosomes ( de Oliveira et al, 2008), as reported in Amazon basin, and simple sex chromosome systems have already been identified in Ancistrus (Mariotto et al, 2004;Mariotto & Miyazawa, 2006;de Oliveira et al, 2006de Oliveira et al, , 2007Mariotto, 2009) according to each locality, a fixed pattern of sex heterogamety cannot be defined, thereby confirming the recurrence and the homoplasic character of sex chromosomes in the tribe Ancistrini.…”

Section: Discussionmentioning

confidence: 99%

“…1 "Balbina", Ancistrus sp. 2 "Barcelos" (de Oliveira et al, 2007); Ancistrus sp. 06, Ancistrus tombador, Lasiancistrus cf.…”

Section: Discussionmentioning

confidence: 99%

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Diversity and chromosomal evolution in the genus Ancistrus Kner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil

2013

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Cytogenetic analyses were carried out in 117 specimens of seven species of the genus Ancistrus from three hydrographic in Mato Grosso State: Paraguay, Araguaia-Tocantins and Amazon basins. Conventional cytogenetic techniques were used to obtain mitotic chromosomes. C-banding was performed to detect heterochromatic regions and silver nitrate staining was used to identify nucleolar organizer regions (Ag-NORs). The counted and paired chromosomes revealed diploid numbers ranging from 2n = 40 to 2n = 54 with karyotype formulae varying from FN = 80 to FN = 86. Single marks in distinct chromosomes identified the nucleolar organizer regions. The constitutive heterochromatin was scarce in the diploid number from 2n = 50 to 2n = 54 and conspicuous blocks were observed in a single species with 2n = 40 chromosomes. These data corroborate the hypotheses of reduction of diploid number in species with derived features such as presence of sex chromosomes and polymorphisms, besides allowing inferences about the evolutionary mechanisms and the ancestor karyotype that favored the diversification of this important genus in the tribe Ancistrini. Foram realizadas análises citogenéticas de 117 espécimes do gênero Ancistrus de três bacias hidrográficas do estado de MatoGrosso: Paraguai, Araguaia-Tocantins e Amazônica, utilizando as técnicas de citogenética convencional para obtenção de cromossomos mitóticos, visualização de regiões heterocromáticas e regiões organizadoras de nucléolos. Os cromossomos pareados revelaram uma variação no número diploide de 2n = 40 a 2n = 54 e número fundamental de NF = 80 a NF = 86. As regiões organizadoras de nucléolos foram evidenciadas em um único par de cromossomos para todas as espécies e a heterocromatina é escassa nas espécies com números diploides elevados (2n = 50 a 2n = 54). Os blocos heterocromáticos mais evidentes foram observados nos pares portadores das AgRONs e em cromossomos da espécie com 2n = 40. Estes dados contribuem para a hipótese de redução do número diploide nas espécies que apresentam polimorfismos cromossômicos e cromossomos sexuais, além de contribuir para inferências sobre os mecanismos de evolução cariotípica que favoreceu a diversificação do gênero Ancistrus, o mais representativo na tribo Ancistrini.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Diversity and chromosomal evolution in the genus Ancistrus Kner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil

2013

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“…However, as new studies were performed in Ancistrini it was pointed out that the chromosomal diversity go beyond the previously supposition for this group. Indeed, the Ancistrini tribe is characterized by a chromosomal diversification, especially in the genus Ancistrus where distinct karyotypic structures indicate a probable species complex (Mariotto & Miyazawa, 2006), besides the occurrence of simple and multiple sex chromosome systems (Mariotto et al, 2004;Mariotto & Miyazawa, 2006;de Oliveira et al, 2007de Oliveira et al, , 2008, as well as simple or multiple and interstitial or terminal NOR systems (Alves et al, 2003;Mariotto et al, 2004;Souza et al, 2004;Mariotto & Miyazawa, 2006;de Oliveira et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Chromosome polymorphism in Ancistrus cuiabae Knaack, 1999 (Siluriformes: Loricariidae: Ancistrini)

et al. 2009

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Cytogenetic and FISH analyses were performed in 30 Ancistrus cuiabae specimens from a bay near the town of Poconé, in the Pantanal of Mato Grosso, Brazil. The observed diploid number was 2n = 34 chromosomes for both sexes and three distinct katyotypic formulae were found, namely cytotype A (20m, 8sm, 6st, Fundamental Number/FN = 68; 6 males and 11 females), cytotype B (19m, 8sm, 6st, 1a, FN = 67; 8 males and 4 females) and cytotype C (18m, 8sm, 6st, 2a, FN = 66; a single male). NORs's analyses showed that these regions were located in distinct sites on the NOR-bearing chromosome pair, according to cytotypes. Thus, in cytotype A, NORs were located in the terminal region of the short arm of the second metacentric chromosome pair; in cytotype B, they were detected in the short arm of the metacentric chromosome and interstitially on the acrocentric chromosome and, in cytotype C, NORs were observed in the interstitial region of the acrocentric chromosome pair. C-positive heterochromatic bands were adjacent to the rDNA sites in the corresponding chromosomes. Thus, the chromosomal polymorphism of A. cuiabae was probably originated through a pericentric inversion in chromosome pair nº 2 involving the NOR sites, which represents a novelty in the Ancistrini tribe. The results also broaden the knowledge of the chromosomal evolution in Ancistrus, the most derived genus of the Ancistrini tribe.Foram analisados, com técnicas convencionais de citogenética e FISH, 30 exemplares da espécie Ancistrus cuiabae da baía Arrombado, próximo a Poconé, Pantanal do Mato Grosso. Foram observadas metáfases com número diploide 2n = 34 cromossomos para ambos os sexos e três fórmulas cariotípicas distintas, aqui denominadas de citótipo A, verificado em 06 machos e 11 fêmeas (20m, 8sm, 6st, Número Fundamental, NF = 68); citótipo B, em 08 machos e 04 fêmeas (19m, 8sm, 6st, 1a, NF = 67) e citótipo C em apenas 01 macho (18m, 8sm, 6st, 2a, NF = 66). As NORs confirmaram os distintos citótipos verificados, além de evidenciar que os cromossomos portadores de rDNA são os que representam o polimorfismo na espécie Ancistrus cuiabae. No citótipo A, as NORs foram verificadas na região terminal do braço curto do segundo par de cromossomos metacêntricos; no citótipo B, foram evidenciadas no segundo par, heteromórfico, no braço curto do cromossomo metacêntrico e intersticial no seu homólogo acrocêntrico; no citótipo C as NORs foram observadas na região intersticial num par de cromossomos acrocêntricos. A análise da heterocromatina constitutiva evidenciou blocos discretos adjacentes ao rDNA no segundo par de cromossomos de ambos os citótipos. Uma provável inversão pericêntrica é a hipótese proposta para a origem deste polimorfismo na espécie Ancistrus cuiabae. Estes resultados ampliam o conhecimento sobre o gênero Ancistrus, o mais derivado da tribo, contribuem para o conhecimento sobre este grupo de peixes e para inferir sobre a evolução cromossômica dos Ancistrini.

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“…In most species of Neotropical fishes with sex chromosomes, addition and/or deletion of heterochromatin or chromosomal rearrangements such as pericentric inversions or Robertsonian translocations involving one of the homologous chromosomes have been suggested important mechanisms in the origin of simple sex chromosome systems (Almeida-Toledo et al, 2000). Multiple sex chromosome systems are those where chromosomal rearrangements are cited as the fundamental mechanisms in the origin of the complex systems (Oliveira et al, 2007). In the case of H. cf.…”

Section: Discussionmentioning

confidence: 99%

Karyotype structure of Hypostomus cf. plecostomus (Linnaeus, 1758) from Tapajós River basin, Southern Amazon: occurrence of sex chromosomes (ZZ/ZW) and their evolutionary implications

Oliveira¹,

Ribeiro²,

Dutra³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.Hypostomus is a group of fish with numerical and structural karyotypic variability. Among them, only six species, three of which belong to the Amazon basin, show a sex chromosome. In this study, we present the karyotype structure of Hypostomus cf. plecostomus from the Teles Pires river basin in the municipality of Alta Floresta, MT. The species has 2n = 68 and the karyotype formula 14m+ 24sm+ 14st+ 16a [fundamental number (FN) = 120] in males and 15m+ L.C. Oliveira et al. 6626©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6625-6634 (2015) 24sm+14st+15a (FN = 121) in females and sex chromosomes ZZ/ZW. Argyrophilic nucleolar organizer regions (AgNORs) were identified in two pairs of chromosomes at different positions: short arm of the pair 21and long arm of the pair 27, matching the signals displayed by 18S FISH and indicating multiple NORs. Analysis of band C detected few blocks of constitutive heterochromatin in the pericentromeric regions of most chromosomes and the telomeric regions of some pairs, including the nucleolar pair 21. However, large blocks on the long arm of the nucleolar pair 27 still stood out. GC-rich heterochromatin (CMA 3 ) was visualized only coincidently with nucleolar sites. Mapping of 5S rDNA sites with FISH revealed markings in eight chromosomes, demonstrating synteny between the 18S and 5S sites. The data obtained for H. cf. plecostomus are important for taxonomic studies of this Amazon complex "H. plecostomus group". The occurrence of sex chromosomes in Amazon species of Hypostomus suggests an evolutionary event that is independent of other species in the group.

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Karyotype characterization and ZZ/ZW sex chromosome heteromorphism in two species of the catfish genus Ancistrus Kner, 1854 (Siluriformes: Loricariidae) from the Amazon basin

Cited by 41 publications

References 30 publications

Diversity and chromosomal evolution in the genus Ancistrus Kner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil

Diversity and chromosomal evolution in the genus Ancistrus Kner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil

Chromosome polymorphism in Ancistrus cuiabae Knaack, 1999 (Siluriformes: Loricariidae: Ancistrini)

Karyotype structure of Hypostomus cf. plecostomus (Linnaeus, 1758) from Tapajós River basin, Southern Amazon: occurrence of sex chromosomes (ZZ/ZW) and their evolutionary implications

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