Somatic chromosome numbers of seven Scorzonera L. species collected from different localities in Iran were counted. Except for S. mucida with 2n=28, the other species exhibited a somatic number of 2n=14. The chromosome numbers support the basic number (x=7) of Scorzonera. The chromosome numbers in S. tunicata, S. kandavanica, S. persepolitana, S. mucida, and S. raddeana are reported for the first time. Chromosome numbers in S. luristanica and S. songorica are different from those of the previous reports.