Background
Species of the
Festuca
and
Lolium
genera, as well as intergeneric
Festuca
×
Lolium
(
Festulolium
) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide.
Festulolium
hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these issues in allotetraploid
Festuca pratensis
×
Lolium perenne
hybrids.
Results
Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and molecular markers – inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental genomes (
Festuca
and
Lolium
) in hybrid karyotypes. The separate analysis of
F. pratensis
and
L. perenne
chromosomes in hybrid plants (F
2
-F
3
generations of
F. pratensis
×
L. perenne
) revealed the asymmetrical level of rearrangements. Recognized structural changes were mainly located in the distal part of chromosome arms, and in chromosomes bearing ribosomal DNA, they were more frequently mapped in arms without this sequence. Based on the ISSR markers distribution, we found that the tetrasomic type of inheritance was characteristic for the majority of ISSR loci, but the disomic type was also observed. Nonetheless, no preference in the transmission of either
Festuca
or
Lolium
alleles to the following generations of allotetraploid
F. pratensis
×
L. perenne
hybrid was observed.
Conclusion
Our study reports cytogenetic and molecular genotyping of the
F. pratensis
×
L. perenne
hybrid and its following F
2
-F
3
progenies. The analysis of 137 allotetraploid
F. pratensis
×
L. perenne
hybrids revealed the higher level of recombination in chromosomes derived from
F. pratensis
genome. The results of ISSR markers indicated a mixed model of inheritance, which may be characteristic for these hybrids.
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