Karyotype Study of Eight Species of European Percid Fishes (Pisces, Percidae)

Abstract

SUMMARY -Karyotypes of eight species of European percid fishes representing allEuropean tribes of Percidae, viz. Percini (Perea fluviatilis, Gymnocephalus cernuus, G. baloni, G. schraetser), Luciopercini (Stizostedion lucioperca, S. volgense) and Romanichthyini (Zingel zingel, Z. streber) were analyzed. Diploid chromosome numbers 2n = 48 have been found in all species analyzed. Their karyotypes are dominated by submetacentric to subtelocentric and acrocentric chromosome and differ in number of metacentric elements: one pair P. fluviatilis, S. lucioperca, S. volgense and G. cernuus to 5 pairs in G. baloni and G. schraetser. In the karyotypes of all species analyzed, the same marker chromosome pairs have been detected, viz. a pair of small metacentrics and a pair of large satellited (i.e. NORs carrying) subtelocentrics, respectively. Karyology of Percidae is further reviewed and discussed. It is postulated that chromosomal evolution of the family has been connected with rearrangements changing the centromere position on particular chromosomes but not with gross, i.e. numerical, karyotype changes.

Characterization of a Y‐specific duplication/insertion of the anti‐Mullerian hormone type II receptor gene based on a chromosome‐scale genome assembly of yellow perch, Perca flavescens

“…Genome sizes are both very close to the 873‐Mbp GenomeScope (Vurture et al, ) estimation based on short‐read analysis with a repeat length of 266 Mbp (30.5%) and slightly lower than the estimation of Perca flavescens genome sizes based on C‐values (900 and 1,200 Mbp records in the Animal Genome Size Database [http://www.genomesize.com/index.php]). The 24 chromosome‐length scaffolds obtained after Hi‐C integration are consistent with the diploid chromosome (Chr) number of yellow perch (2 n = 48; Ráb, Roth, & Mayr, ). The genome completeness of these assemblies was estimated using busco version3.0 (Simão et al, ) based on the Actinopterygii database.…”

“…After Hi-C integration, the genome assembled into 269 fragments with a total length of 877.4 Mb, including 24 chromosome-length scaffolds representing 98.78% of the complete genome sequence (N50 = 37.4 Mb, L50 = 11; see Table 1). Genome sizes are both very close to the 873-Mbp GenomeScope (Vurture et al, 2017) Ráb, Roth, & Mayr, 1987). The genome completeness of these assemblies was estimated using busco version3.0 (Simão et al, 2015) based on the Actinopterygii database.…”

An ultra-high density SNP-based linkage map for enhancing the pikeperch (Sander lucioperca) genome assembly to chromosome-scale

“…Additionally, 7 SNPs were found to be duplicated; they mapped to the same physical position because of collapsing or overlapping contigs in the target assembly. 32,33 . The number of linkage groups for the female, male and sex-averaged maps built in this study was chosen corresponding to the number of chromosome pairs from microscopic observations 32,33 .…”

Sympatric occurrence of triploid, aneuploid and tetraploid weatherfish Misgurnus fossilis (Cypriniformes, Cobitidae)

“…Karyotyping of specimens analysed that were left alive was done by non‐invasive method from cells of regenerating fin margins, according to the method of Völker & Ráb (2011), and from leukocyte culture derived from peripheral blood following the protocol by Fujiwara et al (2001). Other specimens were karyotyped using direct chromosome preparation from the head kidney (Ráb et al , 1987).…”