Karyotypes of eight species of Leptodactylus (Anura, Leptodactylidae) with a description of a new karyotype for the genus

Amaro, Renata Cecília; Skuk, Gabriel; Sá, Rafael O. de; Trefaut, Miguel

doi:10.11606/issn.2316-9079.v5i2p119-133

Cited by 22 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, an explanation for differences among species in their susceptibility to molecular reorganization of rDNA clusters is still lacking. Results of the present study contrast with the major pattern of only one chromosome pair with NOR that is found in the other Phyllomedusa and related species studied so far; however, multiple NORs have been described in several other anuran species (e.g., Wiley et al, 1989;Lourenço et al, 1998Lourenço et al, , 2000Veiga-Menoncello et al, 2003;Amaro-Ghilardi et al, 2006;Silva et al, 1999, indicating that this variation is common in this group.…”

Section: Discussioncontrasting

confidence: 99%

Molecular cytogenetics of nucleolar organizer regions in Phyllomedusa and Phasmahyla species (Hylidae, Phyllomedusinae): a cytotaxonomic contribution

Barth¹,

Souza²,

Solé³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Chromosome numbers, morphology, and nucleolus organizer region (NOR) locations are useful cytological characters for taxonomic and evolutionary studies. In this study, we provide the first cytogenetic analysis of Phyllomedusa bahiana and Phasmahyla spectabilis, and report new cytogenetic data on variation in NOR numbers and positions in Phyllomedusa rohdei and Phyllomedusa nordestina using conventional staining, AgNOR-banding, and 45S rDNA fluorescence in situ hybridization. All 4 species showed 2n = 26 chromosomes. P. spectabilis and P. bahiana had only 1 pair of NOR-carrying chromosomes. P. bahiana showed an NOR length polymorphism, and a rare homomorphic self-compatibility for both NOR lengths in anurans. Variation in the number of NOR-bearing chromosomes was found between the sampled populations of P. nordestina, ranging from 3 to 4. This study also clarified previous conflicting results concerning the occurrence of inter-and intrapopulation NOR variation in P. rohdei. The variation, confirmed by 45S

show abstract

Section: Discussioncontrasting

confidence: 99%

Molecular cytogenetics of nucleolar organizer regions in Phyllomedusa and Phasmahyla species (Hylidae, Phyllomedusinae): a cytotaxonomic contribution

Barth¹,

Souza²,

Solé³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Such differences in the quantity and distribution of the heterochromatin have been important for the definition of speciesand subspecies-level differentiation in amphibians (Matsui et al 1985). The heterochromatin distribution pattern observed in the specimens of L. latrans from Tobias Barreto was similar to that described by Amaro-Ghilardi et al (2006) in specimens from Igarassu (Pernambuco, Brazil), which presented C-positive pericentromeric blocs on the short arms of chromosomes 4, 7 and 8.…”

Section: Discussionsupporting

confidence: 55%

“…While both the species analyzed in the present study had been previously karyotyped, beginning in the 1970s (Denaro 1972, Bianchi et al 1973, Bogart 1974, and revised more recently (Amaro- Ghilardi et al 2006, Silva et al 2006, this is the first cytogenetic study of populations from the Caatinga, the least well protected Brazilian biome (Leal et al 2005).…”

Section: Discussionmentioning

confidence: 95%

“…Approximately 40 species of Leptodactylus have been karyotyped (Zaracho and Hernando 2011), and most share very similar features, with the emphasis on metacentric and submetacentric chromosomes (Silva et al 2004, Amaro-Ghilardi et al 2006, Gazoni et al 2012. A number of cytogenetic studies have reinforced the conservative nature of the karyotypes of the representatives of this genus (Denaro 1972, Bianchi et al 1973, Bogart 1974, Silva et al 2000, Amaro-Ghilardi et al 2006, Arruda and Morielle-Versute 2008, Gazoni et al 2012. The taxonomic position of a number of species groups is, however, still controversial (Angulo et al 2003, Heyer 2005, Lavilla et al 2010.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytogenetic study of Leptodactylus fuscus and L. latrans (Anura: Leptodactylidae) from the semiarid Brazilian Caatinga scrublands

Oliveira¹,

Moares²,

Dertônio³

et al. 2014

Phyllomedusa

View full text Add to dashboard Cite

Cytogenetic study of Leptodactylus fuscus and L. latrans (Anura: Leptodactylidae) from the semiarid Brazilian Caatinga scrublands. Frogs in the family Leptodactylidae have diploid chromosome numbers ranging from 2n = 18 to 2n = 26, although there is a predominance of 2n = 22 karyotypes. In the present study, 52 specimens of Leptodactylus fuscus and Leptodactylus latrans were obtained from three municipalities (Carira, Poço Redondo, and Tobias Barreto) of the Brazilian state of Sergipe for karyotype analysis using conventional Giemsa staining techniques, and C-and Ag-NOR banding of the chromosomes obtained from the intestinal epithelium. The results of this study show that the individuals analyzed have highly similar karyotypes, with no evidence of any pronounced species-specific markers. However, some differences were observed in the chromosome morphology and C-bands in comparison with the karyotypes described previously for these species, which may represent intraspecific geographic variation in both taxa.Keywords: amphibians, chromosomes, C-bands, Ag-NOR. 126Phyllomedusa -12(2), December 2013 Resumo Análise citogenética de Leptodactylus fuscus e L. latrans (Anura: Leptodactylidae) de áreas de Caatinga do Brasil. A família Leptodactylidae apresenta números diplóides que variam de 2n = 18 a 2n = 26, porém, na maioria dos gêneros descritos na literatura, há uma predominância de cariótipos com 2n = 22. Neste trabalho foram analisados citogeneticamente 52 exemplares de Leptodactylus fuscus e Leptodactylus latrans coletados pela primeira vez no estado de Sergipe, norte do Brasil, nos municípios de Carira, Poço Redondo e Tobias Barreto, por meio de técnicas de coloração convencional, Banda C e Ag-RON, empregadas em preparações do epitélio intestinal. Os dados mostraram que as espécies aqui analisadas apresentaram padrões cariotípicos muito similares, sem evidência de marcadores específicos das espécies. Contudo, algumas variações de morfologia cromossômica e bandas C observadas divergiram de cariótipos já descritos na literatura e podem indicar diferenças cromossômicas geográficas intraespecíficas para ambas as espécies.

show abstract

“…Despite differences in position, the occurrence of a secondary constriction on chromosome 8, carrier of the NORs in species of Leptodactylus was referred to by Baldo (2002) and Amaro-Ghilardi et al (2006). In the present study, only on chromosome 8 of L. labyrinthicus was the secondary constriction not observed, probably due to differences in the degree of chromosome condensation in preparations.…”

Section: Cytogenetic Analysiscontrasting

confidence: 46%

Cytogenetic and random ampliied polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia)

Arruda

Morielle‐Versute²

2008

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Cytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of São Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region (NOR) location did not differ between the populations from the two environments. The in situ hybridization with an rDNA probe confirmed the location of the NORs on chromosome 8 revealing an in tandem duplication of that region in one of the chromosomes of L. fuscus. DAPI showed that part of the C-band-positive heterochromatin is rich in AT, including that in the proximity the NORs in L. podicipinus and L. ocellatus. The molecular analyses showed that the two populations (urban and rural) of L. podicipinus and L. fuscus are similar from a genetic point of view. The urban and rural populations of species L. ocellatus and L. labyrinthicus showed differences in genetic structures, probably due to urbanization which interferes with the dispersion of those frogs. The marked differences observed between the two populations of L. ocellatus can be representing the cryptic condition of the species. Unweighted pair-group method of analysis and genetic distance analysis detected the genetic proximity between L. ocellatus and L. fuscus. The results indicate that there was no reduction in the genetic diversity in the populations from the urban environment; however, the survival of these frogs would not be guaranteed in the case of an increase in human impact especially for populations of L. labyrinthicus and L. ocellatus.

show abstract

Karyotypes of eight species of Leptodactylus (Anura, Leptodactylidae) with a description of a new karyotype for the genus

Abstract: Karyotypes of eight species of Leptodactylus (Anura, Leptodactylidae) with a description of a new karyotype for the genus. Eight species of the Neotropical genus Leptodactylus were karyologically studied:

Cited by 22 publications

References 36 publications

Molecular cytogenetics of nucleolar organizer regions in Phyllomedusa and Phasmahyla species (Hylidae, Phyllomedusinae): a cytotaxonomic contribution

Molecular cytogenetics of nucleolar organizer regions in Phyllomedusa and Phasmahyla species (Hylidae, Phyllomedusinae): a cytotaxonomic contribution

Cytogenetic study of Leptodactylus fuscus and L. latrans (Anura: Leptodactylidae) from the semiarid Brazilian Caatinga scrublands

Cytogenetic and random ampliied polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia)

Contact Info

Product

Resources

About