Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná

Noleto, Rafael Bueno; Kantek, Daniel Luis Zanella; Swarça, Ana Cláudia; Dias, Ana Lúcia; Fenocchio, Alberto Sérgio; Cestari, Marta Margarete

doi:10.1590/s1415-47572006000200011

Cited by 13 publications

(16 citation statements)

References 13 publications

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“…Our data confirm that some species of the Testudinidae have a relatively smaller number of microchromosomes in relation to the family Trionychidae (Matsuda et al, 2005;Noleto et al, 2006;Rohilla et al, 2006). The mechanisms responsible for reducing the loss of microchromosomes can be numerical, translocation of microchromosomes to acrocentric chromosomes and formation of metacentric chromosomes during the evolution of Testudines (Bickham, 1975).…”

Section: Resultssupporting

confidence: 84%

Simple method for culture of peripheral blood lymphocytes of Testudinidae

Silva¹,

Silva²,

Venâncio³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We developed and optimized a simple, efficient and inexpensive method for in vitro culture of peripheral blood lymphocytes from the Brazilian tortoise Chelonoidis carbonaria (Testudinidae), testing various parameters, including culture medium, mitogen concentration, mitotic index, culture volume, incubation time, and mitotic arrest. Peripheral blood samples were obtained from the costal vein of four couples. The conditions that gave a good mitotic index were lymphocytes cultured at 37°C in minimum essential medium (7.5 mL), with phytohemagglutinin as a mitogen (0.375 mL), plus streptomycin/penicillin (0.1 mL), and an incubation period of 72 h. Mitotic arrest was induced by 2-h exposure to colchicine (0.1 mL), 70 h after establishing the culture. After mitotic arrest, the cells were hypotonized with 0.075 M KCl for 2 h and fixed with methanol/acetic acid (3:1). The nonbanded mitotic chromosomes were visualized by Giemsa staining. The diploid chromosome number of C. carbonaria was found to be 52 in females and males, and sex chromosomes were not observed. We were Method for peripheral blood lymphocyte culture able to culture peripheral blood lymphocytes of a Brazilian tortoise in vitro, for the preparation of mitotic chromosomes.

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Section: Resultssupporting

confidence: 84%

Simple method for culture of peripheral blood lymphocytes of Testudinidae

Silva¹,

Silva²,

Venâncio³

et al. 2011

Genet. Mol. Res.

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show abstract

“…Se analizaron un total de 917 metafases mitóticas, utilizando 225 para la medición y el armado de los cariogramas (Fig. 1) En el cariotipo de las tortugas puede darse además de los macrocromosomas, presencia o ausencia de microcromosomas (Noleto et al, 2006). En nuestro estudio hallamos 16 pares de macrocromosomas y 9 pares de microcromosomas.…”

Section: Resultados Y Discusiónunclassified

ESTUDIOS CITOGENÉTICOS EN LA TORTUGA PINTADA (Trachemys dorbigni: TESTUDINATA, EMYDIDAE)

Salas¹,

Imhof²,

Amavet³

2014

Natura

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“…For example, the chromosome number in the order Chelonia ranges from 2n=26 in Podocnemis dumeriliana (Ayres et al 1969) to 2n=96 in Platemys platycephala (Bull andLegler 1980, Bickham et al 1985). Moreover, while karyotypic studies have frequently been published for turtles from the suborder Cryptodira, information about Pleurodires is scarce and fragmented and mainly based on conventional staining techniques (Noleto et al 2006).…”

Section: Resultsmentioning

confidence: 99%

First Cytogenetic Study of Malayan Snail-eating Turtle, <i>Malayemys macrocephala</i> (Testudines, Geoemydidae) in Thailand

et al. 2013

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SummaryThe first cytogenetics of the Malayan snail-eating turtle (Malayemys macrocephala) from the Chi river basin, Khon Kaen Province, Thailand, were studied. Blood samples were taken from two male and two female turtles. Standard T-lymphocyte cell culture at 26 C for 96 h was applied. The mitotic chromosomes were harvested by colchicine-hypotonic-fixation-air drying technique. Conventional staining and GTG-banding techniques were applied to stain the chromosomes with 20% Giemsa s solution. Results showed that the number of diploid chromosomes was 2n=50, while the fundamental number (NF) was 40 in both males and females. The types of macrochromosomes were 4 metacentric, 8 submetacentric, 6 acrocentric, 4 telocentric chromosomes, and 28 microchromosomes. The GTG-banding technique showed that each chromosome pairs could be clearly differentiated and the numbers of bands in the M. macrocephala was 99. There is no observation of strangely size chromosomes related to sex. The karyotype formula is as follows:

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Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná

Cited by 13 publications

References 13 publications

Simple method for culture of peripheral blood lymphocytes of Testudinidae

Simple method for culture of peripheral blood lymphocytes of Testudinidae

ESTUDIOS CITOGENÉTICOS EN LA TORTUGA PINTADA (Trachemys dorbigni: TESTUDINATA, EMYDIDAE)

First Cytogenetic Study of Malayan Snail-eating Turtle, <i>Malayemys macrocephala</i> (Testudines, Geoemydidae) in Thailand

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