Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

Costa, Kleber França; Brito, Rute Magalhães; Miyazawa, Carlos Suetoshi

doi:10.1590/s1415-47572004000200010

Cited by 15 publications

(17 citation statements)

References 10 publications

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“…However, most previous studies showed numeric variation as the main source of polymorphism (Brito et al, 1997;Araújo et al, 2002;Scher and Pompolo, 2003;Brown Jr. et al, 2004). The chromosome number 2n = 34 determined for Scaptotrigona xanthotricha agrees with previous studies on other Scaptotrigona species (Hoshiba and Imai, 1993;Rocha et al, 2003) and corroborates the numerical stability in the tribe Meliponini (Brito et al, 2003;Rocha et al, 2003;Costa et al, 2004).…”

Section: Discussionsupporting

confidence: 86%

“…The numeric stability observed in the karyotypes of the tribe Meliponini, with 2n = 34 as the predominant number, indicates that the differences observed are allied to structural chromosome changes, especially in the variable heterochomatin regions along the chromosome segments (Hoshiba and Imai, 1993;Rocha et al, 2003;Costa et al, 2004). Karyotype variation produced by structural chromosome rearrangements frequently occurs in heterochromatic regions (John, 1988;Sumner, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Duarte¹,

Martins²,

Waldschmidt³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Scaptotrigona xanthotricha has a wide geographic distribution in the Brazilian Atlantic rainforest. One population from southeast and two from northeast Brazil were analyzed and were found to have chromosome polymorphisms. Although the chromosome number 2n = 34 is conserved in this species, karyotypic analysis revealed clear differences between the three populations. Congruent and ubiquitous multiple nucleolus organizer regions, heterochromatin and CMA 3 -positive blocks were found. The variations suggest that this species is in a process of genetic differentiation. This differentiation process might have been enhanced by restricted nesting preferences, combined with recent extensive fragmentation of the Atlantic rainforest, which limits gene flow between populations.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Duarte¹,

Martins²,

Waldschmidt³

et al. 2009

Genet. Mol. Res.

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“…T. spinipes Tarelho, 1973;Brito and Pompolo, 1997), T. cilipes , T. recursa (Tarelho, 1973;Costa et al, 2004), T. fuscipennis (Tarelho, 1973), T. branneri, T. hyalinata and T. chanchamayoensis (Costa et al, 2004). We also found another karyotypic difference, a large metacentric chromosome pair in the karyotype of T. fulviventris which was absent, or has not been reported, in the above cited species.…”

supporting

confidence: 50%

“…In contrast, DAPI + labeling was observed on chromosomes whose heterochromatin was weakly labeled by C banding, indicating a widespread distribution of the AT-rich heterochromatin. Costa et al (2004) found a predominance of GC-rich heterochromatin in T. branneri chromosomes but not in T. recursa. These authors did not perform DAPI staining on these two species.…”

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confidence: 82%

Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus

et al. 2005

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Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric region of the first chromosome pair and in one of the arms of the remaining pairs. Base-specific fluorochrome staining with 4'-6-diamidino-2-phenylindole (DAPI) showed that the heterochromatin was rich in AT base pairs (DAPI + ) except for pair 13, which was chromomycin A 3 (CMA 3 ) positive indicating an excess of GC base pairs. Our data also suggests that there was variation in heterochromatin base composition.

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“…We wish to gain a better understanding of the nature of heterochromatin in the tribe Meliponini to verify if the Minimum Interaction Theory can be applied to stingless bees. Pertinent studies on Meliponini bees include those of Pompolo and Campos (1995) in Leurotrigona, Rocha et al (2003) in Friesomiellita and Tetragonisca angustula, Caixeiro (1999) in Plebeia, and Costa et al (2004) in Trigona. However, rather few species (about 80 out of 400 species) of stingless bees have been studied.…”

Section: General Features Of P Helleri and P Seridoensis Karyotypesmentioning

confidence: 99%

Cytogenetic Characterization of Two Partamona Species (Hymenoptera, Apinae, Meliponini) by Fluorochrome Staining and Localization of 18S rDNA Clusters by FISH

Brito¹,

Pompolo²,

Magalhães³

et al. 2005

cytologia

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SummaryThe tribe Meliponini comprises several hundred species of stingless bees which are major pollinators of many tree species. In the present work we studied two Partamona species: Partamona helleri and Partamona seridoensis. Both species presented similar karyotypes with 2nϭ34 chromosomes for females, pericentromeric heterochromatic blocks and terminal GC rich heterochromatic segments in chromosome pairs 2, 9, 10 and 15, as shown by sequential C banding-DA/CMA 3 staining. The heterochromatin is heterogeneous: pericentromeric and the terminal blocks behave quite differently. FISH with 18S rDNA probe showed that NORs were in terminal blocks. In addition, B chromosomes were observed in P. helleri. The results of the different treatments employed such as C banding and fluorochrome staining led us to believe that their origin was not due to nondisjunction of A genome chromosomes. Supernumerary pericentromeric heterochromatin and the non-homologous portion of a heteromorphic pair behaved in a similar fashion, indicating that may be originated by fission. The FISH assay showed, however that there was no sequence correspondence between these segments. The Meliponini are known as stingless bees and are spread over the Pantropical region. This tribe presents a great diversity comprising several hundred species. However, the precise number is not known because of the abundance of cryptic species (Michener 2000). Meliponini are believed to pollinate from 40 to 90% of tree species depending on the ecosystem (Kerr et al. 1996). According to Kerr et al. (1996) almost 100 species of this tribe are already endangered, and the destruction of native vegetation in Brazil endangers bee species that nest in tree holes. As a consequence, many Meliponini are frequently observed nesting in wall crevices and even in plant vases in urban areas. Kerr and Silveira (1972) hypothesized that the different bee chromosome numbers resulted from polyploidization of a basic number, nϭ8 of bee species. Alternatively, the Minimum Interaction Theory (Imai et al. 1986(Imai et al. , 1988(Imai et al. , 1994, based on ants, has also been proposed to explain the karyotype evolution of Meliponini. According to this theory, primitive karyotypes had a small number of large chromosomes and, with time the chromosomes got smaller and increased in number by centric fission. This process would prevent deleterious interactions such as translocations within the interphase nucleus. Key wordsFor Partamona, seven species have been studied. All presented 2nϭ34 chromosomes for females: P. pearsoni and P. helleri (cited as cupira) (Tarelho 1973), P. mulata, P. aiylae, P. vicina and P. sp. (Brito-Ribon et al. 1999), P. peckolti (Brito et al. 2003). In P. helleri a system of B (or super-

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Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

Cited by 15 publications

References 10 publications

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus

Cytogenetic Characterization of Two Partamona Species (Hymenoptera, Apinae, Meliponini) by Fluorochrome Staining and Localization of 18S rDNA Clusters by FISH

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