Karyotypic, Morphological, and Molecular Evidence for Hynobius Yunanicus as a Synonym of Pachyhynobius Shangchengensis (Urodela: Hynobiidae)

Xiong, Jianghui; Chen, Qin; Zeng, Xiaomao; Zhao, Ermi; Luo, Qing

doi:10.1670/07-054.1

Cited by 9 publications

(10 citation statements)

References 13 publications

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“…Our barcoding corresponds to most of the current taxonomic arrangements. The results are consistent with the inclusion of Hynobius yunanicus and H. mantchuricus as junior synonyms of Pachyhynobius shangchengensis and Hynobius leechii , respectively (Zeng & Fu 2004; Xiong et al. 2007; Nishikawa et al.…”

Section: Discussionsupporting

confidence: 81%

“…Alignment of the 16S sequences was verified using secondary structure (Cannone et al 2002). The accuracy of COI as a barcode was assessed using the latest taxonomy (Xiong et al 2007;Frost 2010). Nucleotide saturation was tested by plotting numbers of transitions and transversions against Kimura-2-parameter distance (K2P) (Kimura 1980) using DAMBE v. 5.2.5 (Xia & Xie 2001.…”

Section: Molecular Methodsmentioning

confidence: 99%

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COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)

Xia

Peng

et al. 2011

Molecular Ecology Resources

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The 5¢ region of the mitochondrial DNA (mtDNA) gene cytochrome c oxidase I (COI) is the standard marker for DNA barcoding. However, because COI tends to be highly variable in amphibians, sequencing is often challenging. Consequently, another mtDNA gene, 16S rRNA gene, is often advocated for amphibian barcoding. Herein, we directly compare the usefulness of COI and 16S in discriminating species of hynobiid salamanders using 130 individuals. Species identification and classification of these animals, which are endemic to Asia, are often based on morphology only. Analysis of Kimura 2-parameter genetic distances (K2P) documents the mean intraspecific variation for COI and 16S rRNA genes to be 1.4% and 0.3%, respectively. Whereas COI can always identify species, sometimes 16S cannot. Intra-and interspecific genetic divergences occasionally overlap in both markers, thus reducing the value of a barcoding gap to identify genera. Regardless, COI is the better DNA barcoding marker for hynobiids. In addition to the comparison of two potential markers, high levels of intraspecific divergence in COI (>5%) suggest that both Onychodactylus fischeri and Salamandrella keyserlingii might be composites of cryptic species.

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Section: Discussionsupporting

confidence: 81%

Section: Molecular Methodsmentioning

confidence: 99%

COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)

Xia

Peng

et al. 2011

Molecular Ecology Resources

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“…The two goals of any conservation program are to maintain the genetic diversity of species for long-term evolutionary success and ensure their survival [20,69]. The number of P. shangchengensis decreases each year because of human activities such as arbitrary arrest or killing [17,19,61].…”

Section: Discussionmentioning

confidence: 99%

“…Hynobius yunanicus differs from P. shangchengensis mainly in having little white spots on deep brown dorsal side, in lacking premaxillary fontanelle on the skull, and in lacking connection between maxillary and pterygoid. However, evidences from karyotypic and phylogenetic analysis rejected the validity of H. yunanicus [20]. Therefore, Hynobius yunanicus is a synonym of P. shangchengensis , and our sampling sites should include Huangbaishan.…”

Section: Introductionmentioning

confidence: 99%

Molecular Phylogeography and Population Genetic Structure of an Endangered Species Pachyhynobius shangchengensis (hynobiid Salamander) in a Fragmented Habitat of Southeastern China

Zhao

Zhang

2013

PLoS ONE

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The salamander Pachyhynobius shangchengensis (Hynobiidae) is a vulnerable species restricted to a patchy distribution associated with small mountain streams surrounded by forested slopes in the Mount Dabieshan region in southeastern China. However, molecular phylogeography and population genetic structure of P. shangchengensis remain poorly investigated. In this study, we explored the genetic structure and phylogeography of P. shangchengensis based on partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and cytochrome c oxidase subunit I genes. Fifty-one haplotypes and four clades were found among 93 samples. Phylogenetic analyses revealed four deeply divergent and reciprocally monophyletic mtDNA lineages that approximately correspond to four geographic regions separated by complicated topography and long distances. The distinct geographic distributions of all lineages and the estimated divergence time suggest spatial and temporal separation coinciding with climatic changes during the Pleistocene. Analysis of molecular variance indicated that most of the observed genetic variation occurred among the four groups, implying long-term interruption of gene flow, and the possible separation of P. shangchengensis into four management units for conservation.

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“…shangchengensis, has been the subject of controversy. The both species have recently been placed in synonymy by Xiong et al (2007) and Nishikawa et al (2009). Our finding that the offspring of uniformly dark brown parent P. shangchengensis possessed small blue spots all over the body, typical of H. yunanicus, confirms the view of Xiong et al and Nishikawa et al that this species has been described based on juveniles of P. shangchengensis.…”

Section: Figurementioning

confidence: 99%

Reproduction, Development, and Growth Response to Captive Diets in the Shangcheng Stout Salamander, <I>Pachyhynobius shangchengensis</I> (Amphibia, Urodela, Hynobiidae)

Pasmans¹,

Janssens²,

Sparreboom³

et al. 2012

Asian Herpetological Research

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Karyotypic, Morphological, and Molecular Evidence for Hynobius Yunanicus as a Synonym of Pachyhynobius Shangchengensis (Urodela: Hynobiidae)

Cited by 9 publications

References 13 publications

COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)

COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)

Molecular Phylogeography and Population Genetic Structure of an Endangered Species Pachyhynobius shangchengensis (hynobiid Salamander) in a Fragmented Habitat of Southeastern China

Reproduction, Development, and Growth Response to Captive Diets in the Shangcheng Stout Salamander, <I>Pachyhynobius shangchengensis</I> (Amphibia, Urodela, Hynobiidae)

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