Karyotypic similarity among Barycholos ternetzi and five species of the genus Eleutherodactylus from southeastern Brazil (Anura, Brachycephalidae)

Campos, João Reinaldo Cruz; Ananias, Fernando; Haddad, Célio F. B.; Kasahara, Sanae

doi:10.1016/j.micron.2006.11.001

Cited by 12 publications

(23 citation statements)

References 33 publications

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“…ternetzi specimens from Uberlândia were very similar to those previously described in Gurinhatã specimens (Campos et al , 2008). These two sampling sites are located in the State of Minas Gerais, not very distant from each other.…”

Section: Discussionsupporting

confidence: 83%

“…Some small karyotype differences may have resulted from the use of different chromosome preparation techniques and from the classification methods used for karyotype description. Moreover, in the Barycholos specimens from Uberlândia, the only heterochromatic block detected on the long arm of pair 4 corresponded to a band on pair 4 of the C-banded karyotype of Barycholos ternetzi (Gurinhatã), as shown by Campos et al (2008), although the authors did not explicitly mention this band. In contrast, the karyotype of Barycholos cf.…”

Section: Discussionmentioning

confidence: 98%

“…Genus Eleutherodactylus ( sensu Hedges et al , 2008) is also highly variable, with 2n = 18, 22, 24, 26, 28, 30 and 32 chromosomes. Within the four Eleutherodactylus subgenera, Eleutherodactylus ( Eleutherodactylus ) have 2n = 18, 22, 26, 28 and 30, Eleutherodactylus ( Euhyas ) 2n = 24-32, Eleutherodactylus ( Pelorius ) 2n = 30, and Eleutherodactylus ( Syhrophus ) 2n = 22 and 30 (Duellman, 1967; Beçak, 1968; Brum-Zorrilla and Sáez, 1968; Bogart, 1970a,b,c, 1973, 1981, 1984, 1991; León, 1970; Beçak and Beçak, 1974; De Lucca and Jim, 1974; De Lucca et al , 1974; DeWeese, 1975; Drewry and Jones, 1976; Savage and DeWeese, 1979, 1980; Green et al , 1980; Miyamoto, 1983, 1984; Kaiser et al , 1994, 1995; Bogart and Hedges, 1995; Savage and Myers, 2002; Siqueira et al , 2004; Campos et al , 2008). Additional cytogenetic studies on Terrarana species could help to improve the current taxonomic and evolutionary knowledge regarding this group.…”

Section: Introductionmentioning

confidence: 99%

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The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species

et al. 2009

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A recent substantial rearrangement of the 882 described eleutherodactyline frog species has considerably improved the understanding of their systematics. Nevertheless, many taxonomic aspects of the South American eleutherodactyline species remain unknown and require further investigation using morphological, cytogenetic and molecular approaches. In this work, the karyotypes of the Brazilian species Ischnocnema juipoca (Atibaia and Campos do Jordão, SP), Barycholos cf. ternetzi (Uberlândia, MG, and Porto Nacional, TO), and Pristimantis crepitans (Chapada dos Guimarães and São Vicente, MT) were analyzed using Giemsa staining, Ag-NOR labeling, and C-banding techniques. All individuals had a diploid number of 22 chromosomes, but the Fundamental Numbers were different among species. The herein described low chromosome number of Pristimantis crepitans is unique within this genus, suggesting that cytogenetically this species is not closely related either to its congeneric species or to Ischnocnema. In addition, karyotype differences, mainly in the NOR position, clearly distinguished the two Barycholos populations, besides indicating the existence of a so far undescribed species in this genus. A taxonomic review could clarify the systematic position of P. crepitans and verify the hypothetic new Barycholos species.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species

et al. 2009

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“…The NORs were associated with CMA 3 + signals in both analyzed species, indicating the presence of GC-rich repetitive DNA interspersed with ribosomal genes, as commonly observed in anurans (Ananias et al 2007, Campos et al 2008). In spite of this correlation between base-specific fluorochrome and rDNA, the mapping of 18S rDNA by FISH is necessary to validate the precise location and number of NORs.…”

Section: Discussionsupporting

confidence: 60%

Cytogenetic analysis of Scinax auratus and Scinax eurydice (Anura, Hylidae) with emphasis on cytotaxonomy

Nogueira

Paim

Diniz

et al. 2015

CCG

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Scinax Wagler, 1830 is a species-rich genus of amphibians with relatively few detailed chromosomal reports. In this work, cytogenetic analyses of Scinax auratus (Wied-Neuwied, 1821) and Scinax eurydice (Bokermann, 1968) were carried out based on conventional (Giemsa staining, Ag-NOR and C-banding) and cytomolecular (base-specific fluorochrome staining and fluorescence in situ hybridization – FISH of ribosomal probes) techniques. Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinax ruber clade. Interpopulation chromosomal variation was observed in Scinax eurydice, indicating the occurrence of cryptic species. The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.

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“…The most illustrative case among animals was described in Ornithorhynchus anatinus [41], in which the multivalent formation was attributed to sequential reciprocal translocations. The same occurred in one specimen of the frogs Haddadus binotatus [39] and Leptodactylus pentadactylus [40], which presented meiotic chain and several odd heteromorphic chromosomes in their karyotypes.…”

Section: Discussionmentioning

confidence: 55%

Comparative karyotype analysis and chromosome evolution in the genus Aplastodiscus (Cophomantini, Hylinae, Hylidae)

et al. 2012

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BackgroundThe frogs of the Tribe Cophomantini present, in general, 2n = 24 karyotype, but data on Aplastodiscus showed variation in diploid number from 2n = 24 to 2n = 18. Five species were karyotyped, one of them for the first time, using conventional and molecular cytogenetic techniques, with the aim to perform a comprehensive comparative analysis towards the understanding of chromosome evolution in light of the phylogeny.ResultsAplastodiscus perviridis showed 2n = 24, A. arildae and A. eugenioi, 2n = 22, A. callipygius, 2n = 20, and A. leucopygius, 2n = 18. In the metaphase I cells of two species only bivalents occurred, whereas in A. arildae, A. callipygius, and A. leucopygius one tetravalent was also observed besides the bivalents. BrdU incorporation produced replication bands especially in the largest chromosomes, and a relatively good banding correspondence was noticed among some of them. Silver impregnation and FISH with an rDNA probe identified a single NOR pair: the 11 in A. perviridis and A. arildae; the 6 in A. eugenioi; and the 9 in A. callipygius and A. leucopygius. C-banding showed a predominantly centromeric distribution of the heterochromatin, and in one of the species distinct molecular composition was revealed by CMA3. The telomeric probe hybridised all chromosome ends and additionally disclosed the presence of telomere-like sequences in centromeric regions of three species.ConclusionsBased on the hypothesis of 2n = 24 ancestral karyotype for Aplastodiscus, and considering the karyotype differences and similarities, two evolutionary pathways through fusion events were suggested. One of them corresponded to the reduction of 2n = 24 to 22, and the other, the reduction of 2n = 24 to 20, and subsequently to 18. Regarding the NOR, two conditions were recognised: plesiomorphy, represented by the homeologous small-sized NOR-bearing pairs, and derivation, represented by the NOR in a medium-sized pair. In spite of the apparent uniformity of C-banding patterns, heterogeneity in the molecular composition of some repetitive regions was revealed by CMA3 staining and by interstitial telomeric labelling. The meiotic tetravalent might be due to minute reciprocal translocations or to non-chiasmatic ectopic pairing between terminal repetitive sequences. The comparative cytogenetic analysis allowed to outline the chromosome evolution and contributed to enlighten the relationships within the genus Aplastodiscus.

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Karyotypic similarity among Barycholos ternetzi and five species of the genus Eleutherodactylus from southeastern Brazil (Anura, Brachycephalidae)

Cited by 12 publications

References 33 publications

The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species

The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species

Cytogenetic analysis of Scinax auratus and Scinax eurydice (Anura, Hylidae) with emphasis on cytotaxonomy

Comparative karyotype analysis and chromosome evolution in the genus Aplastodiscus (Cophomantini, Hylinae, Hylidae)

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