Karyotypic study of five Lutjanid species using conventional and Ag-NORs banding techniques

Phimphan, Sumalee; Supiwong, Weerayuth; Tanomtong, Alongklod; Pinthong, Krit; Sangpakdee, Wiwat; Kaewsri, Sarawut

doi:10.3103/s0095452717040077

Cited by 6 publications

(6 citation statements)

References 15 publications

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“…Conventional staining was carried out using 20% Giemsa's solution for 15 minutes (Phimphan et al 2017). Ag-NOR banding was performed by adding 4 drops of 50% silver nitrate and 2% gelatin on slides (Howell and Black 1980).…”

Section: Chromosome Stainingmentioning

confidence: 99%

A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

et al. 2021

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The cytogenetic comparisons of five Minnow species from Thailand were presented here, i.e., Devario regina, D. laoensis, Rasbora paviana, R. aurotaenia and Esomus metalicus. The mitotic chromosomes were prepared directly from renal cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results revealed that all Minnow fishes studied possessed the same diploid chromosome number (2n) as 50 chromosomes. The fundamental numbers (NF) of D. laoensis, D. regina, R. paviana, R. aurotaenia and E. metalicus are 100, 100, 98, 98, and 98 respectively. Their karyotypes composing of metacentrics-submetacentrics-acrocentrics-telocentrics were as follows: 6-12-32-0 in D. regina, 6-10-34-0 in D. laoensis, 8-16-24-2 in R. paviana, 8-16-24-2 in R. aurotaenia and 8-10-30-2 in E. metalicus. The Ag-NOR banding technique provides the nucleolar organizer regions (NORs) at subtelomeric region of the short arm chromosome in the a submetacentric or acrocentric chromosomes that are located differently in the different chromosome pairs among species.

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Section: Chromosome Stainingmentioning

confidence: 99%

A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

et al. 2021

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“…In fishes, a single NOR carrying chromosome pair is considered as a primitive state ( Milhomem et al 2013 ). Many families such as Chaetodontidae ( Supiwong et al 2017a ), Lutjanidae ( Phimphan et al 2017 ), Notopteridae ( Maneechot et al 2015 ), Scaridae ( Kaewsri et al 2014 ), Serranidae ( Pinthong et al 2013 ), share this character. Also, for fishes the location of NORs in a terminal position, as seen in the studied species, is also considered as a primitive characteristic ( Vitturi et al 1995 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity

Yeesin¹,

Buasriyot²,

Ditcharoen³

et al. 2021

CCG

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Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.

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“…The single NOR-bearing chromosomes in the C B A present result are most likely homologous to that observed in Sphoeroides greeleyi (Brum et al 1995) and S. testudineus (Sá-Gabriel and Molina 2005). A single NORbearing chromosome is a common characteristic found in many fish groups as well as vertebrates (Supiwong et al 2010, 2012a,b, 2013b, Chooseangjaew et al 2017Kasiroek et al 2017;Phimphan et al 2017;Sangpakdee et al 2017;Chaiyasan et al 2018). NOR mapping is frequently used to compare variations, as well as to identify and explain speciation.…”

Section: Discussionmentioning

confidence: 99%

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

et al. 2020

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Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The localization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.

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Karyotypic study of five Lutjanid species using conventional and Ag-NORs banding techniques

Cited by 6 publications

References 15 publications

A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

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