Kawasaki Disease: Issues in Diagnosis and Treatment - A Developing Country Perspective

Singh, Surjit; Sharma, Avinash; Jiao, Fuyong

doi:10.1007/s12098-015-1890-4

Cited by 36 publications

(35 citation statements)

References 32 publications

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“…So far, the diagnosis of KD is based on the clinical features, and a specific laboratory test for the early identification and diagnosis of KD does not exist [3]. Importantly, delay in accurate diagnosis comes out increased mortality and morbidity from complications of KD [4]. microRNAs (miRNAs) are 18 to 23 nucleotides non-coding RNAs.…”

Section: Introductionmentioning

confidence: 99%

A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs

Jiang

Tian

et al. 2017

PLoS ONE

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BackgroundKawasaki disease (KD) is a childhood systemic vasculitis that exhibits a specific preference for the coronary arteries. The aetiology remains unknown and there are no especially diagnostic tests. microRNAs (miRNAs) are 18 to 23 nucleotides non-coding RNAs that are negative regulator of gene expression and play a crucial role in the regulatory network of the genome. Recently, circulating miRNAs have been found presentation in human plasma and displayed some characteristics of the ideal biomarker. However, few researches explored differentially expressed miRNAs in the plasma of KD patients. Our study is to identify circulating miRNAs in KD plasma which can serve as potential biomarkers of KD diagnosis.Materials and methodsThe total of five pairs of acute KD and normal plasma samples were analyzed using ABI miRNAs TLDA Assay chip. Differentially expression of miR-125a-5p in plasma were confirmed by quantitative real-time PCR (qRT-PCR) in independent cohort (acute KD = 30, convalescent KD = 30 and healthy control = 32). After bioinformatics prediction, miR-125a-5p vector and inhibitor were transfected into HUVECs respectively, to observe MKK7 expression as a potential target gene. Flow cytometry was used to analyze apoptosis. The mRNA and protein levels of desired genes including MKK7, Caspase-3, Bax and Bcl2 were detected by qRT-PCR and western blotting.ResultsEighteen miRNAs were differentially expressed in acute KD’s plasma compared with healthy control. miR-125a-5p was significantly increased in plasma of KD patients (p = 0.000), but no variation between acute and convalescent KD (p = 0.357). Moreover, the results from the gain and loss functions of miR-125a-5p in HUVECs have shown that miR-125a-5p remarkably suppressed MKK7 expression, as a novel target gene. Importantly, miR-125a-5p also induced apoptosis in HUVECs through inhibition MKK7 levels to regulate Bax/Bcl2 pathway resulting to activate Caspase-3.ConclusionOur study indicated that the circulating miR-125a-5p levels in KD’s plasma have remarkably evaluated compared with healthy individuals. miR-125a-5p might play a role in the development of KD by regulating target gene MKK7 to induce apoptosis in vascular endothelial cells. Therefore, our findings have suggested that detected miR-125a-5p levels in plasma could be used as a potential biomarker in early KD diagnosis.

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Section: Introductionmentioning

confidence: 99%

A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs

Jiang

Tian

et al. 2017

PLoS ONE

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“…The diagnosis of KD is based solely on clinical features, because there is no specific laboratory test for the early identification and diagnosis of KD . In an important way, a delay in accurate diagnosis may lead to increased mortality and morbidity from complications of KD . Thus, improving the diagnosis and treatment of KD is necessary for reducing mortality and morbidity associated with KD.…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of serum circANRIL and hsa_circ_0123996 in children with Kawasaki disease

Wu¹,

Zhou

Niu

et al. 2019

Clinical Laboratory Analysis

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BackgroundKawasaki disease is a childhood systemic vasculitis that causes coronary artery abnormalities. The etiology remains unknown and there are no specific diagnostic tests. Circular non‐coding RNAs are a special class of endogenous RNAs that display some characteristics of an ideal biomarker. However, few studies have examined the expression of circRNAs in the serum of Kawasaki disease (KD) patients. The aim of this study was to identify circRNAs in the serum that can serve as potential biomarkers for KD diagnosis.MethodsThe cases were children diagnosed with KD (n = 56). The controls comprised healthy children (n = 56). Blood was collected from the patients before and after intravenous immunoglobulin therapy, and from the healthy controls. Levels of circANRIL and hsa_circ_0123996 in the serum were measured by quantitative reverse transcription PCR. Then, the potential relationship between serum circRNA levels and patients’ biochemical parameter levels was investigated. Receiver operating characteristic curves were constructed for evaluating the diagnostic value of these circRNAs.ResultsThe serum levels of circANRIL were lower in patients with KD before therapy than in the controls, but became higher in the patients after therapy than before therapy. The serum levels of hsa_circ_0123996 were higher in patients with KD before therapy than in healthy controls.ConclusionOur study indicated that the circANRIL and hsa_circ_0123996 levels in the serum of patients with KD were significantly different from those in healthy individuals. circANRIL and hsa_circ_0123996 may become potential biomarkers for early KD diagnosis.

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“…KD may be difficult to diagnose and manage in resource limited settings[63] because of limited awareness, inability to gain access to appropriate investigations and treatment, and because of difficulty in distinguishing KD clinically from exanthemata, such as measles, which are endemic in many developing countries. [64]…”

Section: Introductionmentioning

confidence: 99%

Kawasaki disease for dermatologists

Gupta

Singh

2016

Indian Dermatol Online J

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Kawasaki disease (KD) is a systemic vasculitis that mostly affects children below the age of 5. The vasculitis involves arteries of medium size, especially the coronaries. Various etiologies have been proposed including association with micro-organisms, bacterial superantigens, and genetic factors, however, the exact cause remains unknown. There is no specific laboratory test for KD. Diagnosis is clinical and depends upon the presence of fever for ≥5 days and 4 or more of five principal features, viz. polymorphous exanthem, extremity changes, mucosal changes involving the lips and oral cavity, bilateral bulbar conjunctival injection, and unilateral cervical lymphadenopathy. The term “incomplete KD” refers to the presence of fever and less than four principal clinical features. Recognition of this group of patients is important because it is usually seen in infants and risk of coronary abnormalities is increased probably because of delays in diagnosis. However, what appears to be “incomplete” at a given point of time may not actually be so because some of the features may have already subsided and others may evolve over time. Hence, a detailed dermatological examination is warranted in all cases, especially in incomplete KD, to ensure timely diagnosis. Although KD is a self-limiting disease in most patients, coronary artery abnormalities (CAAs) including coronary dilatations and aneurysms may develop in up to 25% of untreated patients. CAAs are the most common cause of morbidity and mortality in patients with KD. Treatment is aimed at reducing inflammation and consists of intravenous immunoglobulin (IVIG) along with aspirin. Despite treatment, some patients may still develop CAAs, and hence, long-term follow up is of utmost importance.

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Kawasaki Disease: Issues in Diagnosis and Treatment - A Developing Country Perspective

Cited by 36 publications

References 32 publications

A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs

A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs

Aberrant expression of serum circANRIL and hsa_circ_0123996 in children with Kawasaki disease

Kawasaki disease for dermatologists

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